##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547487_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1286573
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.569727485342845	31.0	31.0	33.0	30.0	34.0
2	32.011882730323116	31.0	31.0	34.0	30.0	34.0
3	32.091695535348556	33.0	31.0	34.0	30.0	34.0
4	35.83026303210156	37.0	35.0	37.0	35.0	37.0
5	35.76696230995054	37.0	35.0	37.0	35.0	37.0
6	35.821320671271664	37.0	35.0	37.0	35.0	37.0
7	36.097226507940086	37.0	35.0	37.0	35.0	37.0
8	36.09540461365193	37.0	35.0	37.0	35.0	37.0
9	37.95463296680406	39.0	38.0	39.0	35.0	39.0
10	37.43553144671931	39.0	37.0	39.0	35.0	39.0
11	37.10052441641477	39.0	37.0	39.0	33.0	39.0
12	36.52519678246007	39.0	35.0	39.0	33.0	39.0
13	36.281837874726115	39.0	35.0	39.0	32.0	39.0
14	37.34680426217556	40.0	35.0	41.0	32.0	41.0
15	37.502949307967754	40.0	35.0	41.0	33.0	41.0
16	37.59661985755958	40.0	35.0	41.0	33.0	41.0
17	37.51015760473755	39.0	35.0	41.0	33.0	41.0
18	37.39742167758845	39.0	35.0	41.0	32.0	41.0
19	37.36817421164598	39.0	35.0	41.0	32.0	41.0
20	37.260036546701976	39.0	35.0	41.0	32.0	41.0
21	37.11191436475039	39.0	35.0	41.0	32.0	41.0
22	37.06042331060888	39.0	35.0	41.0	32.0	41.0
23	36.98474707614725	39.0	35.0	41.0	32.0	41.0
24	36.835329204017185	38.0	35.0	41.0	31.0	41.0
25	36.70813471136111	38.0	35.0	41.0	31.0	41.0
26	36.65026858172836	38.0	35.0	41.0	31.0	41.0
27	36.62833123343953	38.0	35.0	41.0	31.0	41.0
28	36.601429534118935	38.0	35.0	41.0	31.0	41.0
29	36.55694235772086	38.0	35.0	41.0	31.0	41.0
30	36.49215163072752	38.0	35.0	40.0	31.0	41.0
31	36.437072750632886	38.0	35.0	41.0	31.0	41.0
32	36.309324849814196	38.0	35.0	41.0	30.0	41.0
33	36.2342921855192	38.0	35.0	41.0	30.0	41.0
34	36.10756016176307	38.0	35.0	41.0	30.0	41.0
35	35.93547587272545	38.0	35.0	41.0	30.0	41.0
36	35.79860995062076	38.0	35.0	41.0	29.0	41.0
37	35.729608813491346	38.0	35.0	40.0	29.0	41.0
38	35.590493504838044	38.0	35.0	40.0	28.0	41.0
39	35.51077319359259	38.0	35.0	40.0	28.0	41.0
40	35.3420660934125	38.0	34.0	40.0	27.0	41.0
41	35.274200531178565	38.0	34.0	40.0	27.0	41.0
42	35.22318127304086	37.0	34.0	40.0	27.0	41.0
43	35.09897145362137	37.0	34.0	40.0	27.0	41.0
44	34.966535128593556	37.0	34.0	40.0	26.0	41.0
45	34.84390236698578	37.0	34.0	40.0	26.0	41.0
46	34.78260464038962	37.0	34.0	40.0	26.0	41.0
47	34.70445750066261	36.0	34.0	40.0	25.0	41.0
48	34.577602670038935	36.0	34.0	40.0	25.0	41.0
49	34.46320962743661	36.0	34.0	40.0	24.0	41.0
50	34.37137029923681	36.0	34.0	40.0	24.0	41.0
51	33.973837473660645	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	10.0
13	15.0
14	35.0
15	58.0
16	133.0
17	302.0
18	553.0
19	1117.0
20	1954.0
21	2987.0
22	4391.0
23	6226.0
24	8692.0
25	12456.0
26	16541.0
27	19775.0
28	21743.0
29	24682.0
30	29303.0
31	35683.0
32	44752.0
33	58681.0
34	103831.0
35	156437.0
36	91339.0
37	119549.0
38	183402.0
39	341814.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.65784374458348	23.800359559853966	29.817896069636156	13.723900625926394
2	30.244921974889884	25.668112108679413	30.106259030774	13.980706885656701
3	29.47007282136342	25.916368523200784	30.62002700196569	13.993531653470109
4	26.517111737927035	27.533299703942177	30.313864817620146	15.635723740510644
5	23.47018008305786	33.21506047460968	28.386729707525344	14.928029734807119
6	22.957733451580285	40.90549078831904	25.602278300570585	10.534497459530085
7	89.40930673968752	3.6222585115652204	5.4372351976918525	1.5311995510554006
8	90.41484626212426	2.7322196253146926	5.333782070663693	1.5191520418973505
9	85.861276429709	5.057000263490684	6.5165365665220705	2.565186740278243
10	46.436385653981546	29.5416583435219	12.732351759286104	11.289604243210452
11	37.267298474318984	22.759532494463976	23.452225408119087	16.52094362309795
12	33.88762238909102	21.077078409075895	27.12632707199669	17.908972129836396
13	24.659929906814458	29.395844619776724	27.52164082411181	18.422584649297008
14	19.03024546605595	32.471457119028614	29.320450530206994	19.177846884708448
15	17.89327150499816	25.93836494314742	37.96978484703161	18.19857870482281
16	22.419948187938033	23.035381591250555	36.19483698165592	18.349833239155494
17	22.996596384348187	22.57571082247179	27.21998673996734	27.207706053212682
18	24.952878694018917	24.094862864369144	29.916918822328775	21.035339619283167
19	27.621907190653	25.991840338636052	25.903854658849518	20.482397811861432
20	29.704416305953877	25.82861602101086	25.190408939096343	19.276558733938923
21	26.282224172277825	24.83527945946324	29.61130071904198	19.271195649216953
22	25.988575852283546	22.154281179536646	28.515443740852636	23.34169922732717
23	22.492621872214013	26.673807082847222	27.728547078168127	23.105023966770638
24	22.027277115251138	26.01982165022894	32.25172609715889	19.701175137361034
25	22.358000673105995	25.440841677852717	30.44421109412369	21.7569465549176
26	20.96872855251898	29.031698939741467	27.18221196931694	22.817360538422616
27	20.7076473701842	29.072349567416694	29.72019465665765	20.499808405741454
28	18.719575181509327	27.34465902828677	33.75890835576372	20.176857434440176
29	20.461800457494444	25.162738530965594	33.05346840016073	21.321992611379223
30	21.424124398693273	25.860172722418394	31.810787261974255	20.90491561691408
31	26.891594958078553	25.05555456239172	27.17832567604015	20.87452480348958
32	27.28613145153831	25.440297596793965	27.566410922660435	19.70716002900729
33	25.496337945845283	25.78866492612545	26.492006283358972	22.2229908446703
34	21.411299630879867	26.655930133773985	28.904928053052565	23.027842182293583
35	21.567606346472374	26.2960593763432	29.887771622752844	22.248562654431577
36	27.707405642742387	25.587277208522174	26.82513934304544	19.88017780569
37	22.40665706493141	27.860991953041143	28.615321478066146	21.117029503961298
38	23.102148109745812	29.279799902531767	25.48102595033473	22.1370260373877
39	21.920714953601543	28.26975227989395	27.137053241440633	22.67247952506387
40	24.345373328991048	25.601501041915224	26.24367214297207	23.809453486121658
41	21.05010753373497	24.89551700525349	28.78437523560653	25.27000022540501
42	24.109242149493266	25.382780456297464	26.47552839986538	24.032448994343888
43	22.546641348761398	25.61906708752632	28.600087208421133	23.234204355291148
44	22.129875257758403	27.37846977979485	27.785597863471406	22.70605709897534
45	21.306447438272063	29.520206004634016	25.49579386478653	23.677552692307394
46	23.324754988640365	27.423239878343473	27.02038671727139	22.231618415744773
47	22.985947940769783	27.062125507064117	27.770052690364246	22.181873861801858
48	24.098826883511467	26.092961689698136	28.25218623428286	21.556025192507537
49	22.725410839493758	25.32868325388454	29.596610530455713	22.349295376165983
50	22.103837092803907	27.305096562729048	28.788261528883318	21.802804815583727
51	21.079332459176435	29.14758820525536	26.813558189080606	22.959521146487607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1431.0
1	1991.5
2	2552.0
3	6174.0
4	9796.0
5	7112.0
6	4428.0
7	4413.0
8	4398.0
9	4519.5
10	4641.0
11	4695.0
12	4749.0
13	4616.5
14	4484.0
15	4198.5
16	3913.0
17	3896.0
18	3879.0
19	4058.0
20	4237.0
21	4580.0
22	4923.0
23	5752.0
24	6581.0
25	8059.0
26	10747.5
27	11958.0
28	14282.5
29	16607.0
30	18622.5
31	20638.0
32	24539.0
33	28440.0
34	32311.0
35	36182.0
36	38675.5
37	41169.0
38	45453.5
39	49738.0
40	59913.0
41	70088.0
42	80149.0
43	90210.0
44	96979.0
45	103748.0
46	99477.5
47	95207.0
48	95238.5
49	95270.0
50	90733.0
51	86196.0
52	83570.0
53	80944.0
54	74914.5
55	68885.0
56	67036.0
57	65187.0
58	62229.5
59	59272.0
60	56824.5
61	54377.0
62	49143.5
63	43910.0
64	37237.0
65	30564.0
66	26832.0
67	23100.0
68	19705.5
69	16311.0
70	13791.5
71	11272.0
72	9205.0
73	7138.0
74	5927.0
75	3773.0
76	2830.0
77	2083.0
78	1336.0
79	1051.5
80	767.0
81	613.0
82	459.0
83	363.5
84	268.0
85	184.5
86	101.0
87	83.0
88	65.0
89	55.0
90	45.0
91	30.5
92	16.0
93	10.0
94	4.0
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1286573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.754430505244294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04394100581024	15.386873899698308
2	5.818482557715237	2.1824465354929297
3	1.717602059242853	0.9663794536720373
4	0.9387060894331093	0.7041959247649152
5	0.6173147512862741	0.5788693301430297
6	0.44731630430153113	0.5033497525731466
7	0.36146146171720117	0.4745302704869489
8	0.29719402157520464	0.4458963699364999
9	0.24824906380009992	0.41901928335278377
>10	4.235198177016797	20.503697085166046
>50	2.195771394237902	29.928872477341812
>100	1.0754374472312518	27.04733302619414
>500	0.002494249974251067	0.34748434315998195
>1k	4.157083290418446E-4	0.08271951718481958
>5k	4.157083290418446E-4	0.42833273083260964
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5494	0.4270259052537244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011892057426978492	0.0
2	0.0	0.0	0.0	0.0512213453880969	0.0
3	0.0	0.0	0.0	0.08083490015723943	0.0
4	0.0	0.0	0.0	0.1687428540782373	0.0
5	0.0	0.0	0.0	0.3071726205975098	0.0
6	0.0	0.0	0.0	0.45648400829179536	0.0
7	0.0	0.0	0.0	0.5281472563158095	0.0
8	0.0	0.0	0.0	0.6854644081602832	0.0
9	0.0	0.0	0.0	0.7415047572116001	0.0
10	0.0	0.0	0.0	0.8524972931967327	0.0
11	0.0	0.0	0.0	1.0140893676456757	0.0
12	0.0	0.0	0.0	1.1476224046361925	0.0
13	0.0	0.0	0.0	1.2032741243598304	0.0
14	0.0	0.0	0.0	1.2228610424748538	0.0
15	0.0	0.0	0.0	1.2536404852270333	0.0
16	0.0	0.0	0.0	1.3144221120760347	0.0
17	0.0	0.0	0.0	1.390826637897733	0.0
18	0.0	0.0	0.0	1.4884503250107068	0.0
19	0.0	0.0	0.0	1.5430916084823791	0.0
20	0.0	0.0	0.0	1.609236320053351	0.0
21	0.0	0.0	0.0	1.6929470772354154	0.0
22	0.0	0.0	0.0	1.771605653157652	0.0
23	0.0	0.0	0.0	1.8584254449611488	0.0
24	0.0	0.0	0.0	1.9253474151874788	0.0
25	0.0	0.0	0.0	1.9793668917348646	0.0
26	0.0	0.0	0.0	2.0370394839624337	0.0
27	0.0	0.0	0.0	2.099375628122151	0.0
28	0.0	0.0	0.0	2.1628776602649054	0.0
29	0.0	0.0	0.0	2.2388935567589248	0.0
30	0.0	0.0	0.0	2.3219825070167026	0.0
31	0.0	0.0	0.0	2.4016515191909047	0.0
32	0.0	0.0	0.0	2.469195296341521	0.0
33	0.0	0.0	0.0	2.545988451490899	0.0
34	0.0	0.0	0.0	2.624336123950992	0.0
35	0.0	0.0	0.0	2.720871648946465	0.0
36	0.0	0.0	0.0	2.8014733715070967	0.0
37	0.0	0.0	0.0	2.8921794565873835	0.0
38	7.772586553580713E-5	0.0	0.0	2.9994411510267978	0.0
39	7.772586553580713E-5	0.0	0.0	3.1891699887997027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTACG	30	2.1657052E-6	45.000004	19
GTATTCG	20	7.0336007E-4	45.0	1
CGAAACA	25	3.891066E-5	45.0	31
ATTCGAC	25	3.891066E-5	45.0	35
CAATTCG	20	7.0336007E-4	45.0	1
TTAACGA	25	3.891066E-5	45.0	22
TTACGTC	25	3.891066E-5	45.0	28
TACCGGT	35	1.2120836E-7	45.0	13
TATAGCG	80	0.0	42.1875	1
TATTACG	60	3.6379788E-12	41.250004	1
ACGGGAT	530	0.0	41.179245	5
TAGGGAC	1095	0.0	40.47945	5
TATGGGC	490	0.0	40.408165	4
AACGGGA	615	0.0	40.2439	4
CGTTTTT	2925	0.0	40.230774	1
TACGTAG	135	0.0	40.0	1
TATCCGA	45	1.9286745E-8	40.0	44
GGCGATT	560	0.0	39.776783	8
ATTAACG	120	0.0	39.375004	1
TCACGAC	160	0.0	39.375	25
>>END_MODULE