FastQCFastQC Report
Thu 26 May 2016
SRR1547486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547486_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5247975
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT917211.747740795259124No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65350.12452422124724298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT365600.043.5229761
ATACCCG800.042.18750441
CGCATCG4300.041.33720821
CGGTCTA8250.040.36363631
CTAAGCG1850.040.1351361
CGACGGT8350.039.88023828
CGTAAGG5650.039.823012
TAAGGGA80850.038.9888734
ACGGGTA5000.038.6999975
GTAGGGA86350.038.5639844
TACGGGA22850.038.4026264
TAGGGAC70000.038.2821435
AGGGACC55950.038.2037546
GACCGAT24750.038.09
GCGTAAT301.14047674E-437.50000444
TCACGAC9000.037.525
CTACGCA900.037.523
GGACCGA28150.037.486688
ACGGTCT8900.037.4157330
CTCACGA9100.037.33516324
Produced by FastQC (version 0.11.5)