##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547486_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5247975
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529177825732784	31.0	31.0	33.0	30.0	34.0
2	31.937205302997825	31.0	31.0	34.0	30.0	34.0
3	31.786018607177056	33.0	31.0	34.0	30.0	34.0
4	35.64245980592514	37.0	35.0	37.0	33.0	37.0
5	35.64707091783021	37.0	35.0	37.0	33.0	37.0
6	35.73189030054449	37.0	35.0	37.0	33.0	37.0
7	36.108881616242456	37.0	35.0	37.0	35.0	37.0
8	36.13403341288783	37.0	35.0	37.0	35.0	37.0
9	37.98421238668248	39.0	38.0	39.0	35.0	39.0
10	37.44676051238811	39.0	37.0	39.0	35.0	39.0
11	37.10527946493647	39.0	37.0	39.0	33.0	39.0
12	36.8159463412078	39.0	35.0	39.0	33.0	39.0
13	36.680874623068895	39.0	35.0	39.0	33.0	39.0
14	37.81002805844159	40.0	36.0	41.0	33.0	41.0
15	37.91672445085962	40.0	36.0	41.0	33.0	41.0
16	37.979669682115485	40.0	36.0	41.0	33.0	41.0
17	37.90626346352641	40.0	36.0	41.0	33.0	41.0
18	37.732385539184165	39.0	36.0	41.0	33.0	41.0
19	37.633005111495386	39.0	36.0	41.0	33.0	41.0
20	37.44776146990029	39.0	35.0	41.0	33.0	41.0
21	37.33897112695849	39.0	35.0	41.0	32.0	41.0
22	37.28708158861275	39.0	35.0	41.0	32.0	41.0
23	37.19379760764866	39.0	35.0	41.0	32.0	41.0
24	37.0771806268132	39.0	35.0	41.0	32.0	41.0
25	36.956726737455874	39.0	35.0	41.0	32.0	41.0
26	36.90551498435111	39.0	35.0	41.0	32.0	41.0
27	36.856701680171874	39.0	35.0	41.0	32.0	41.0
28	36.81003606152849	39.0	35.0	41.0	31.0	41.0
29	36.72193312658692	38.0	35.0	41.0	31.0	41.0
30	36.62622821183409	38.0	35.0	41.0	31.0	41.0
31	36.48823879686927	38.0	35.0	41.0	31.0	41.0
32	36.28965400940363	38.0	35.0	41.0	30.0	41.0
33	36.10223714861446	38.0	35.0	41.0	30.0	41.0
34	35.85736498363654	38.0	35.0	41.0	29.0	41.0
35	35.60592075991216	38.0	35.0	41.0	27.0	41.0
36	35.45722092807225	38.0	35.0	41.0	25.0	41.0
37	35.38421867482219	38.0	35.0	41.0	25.0	41.0
38	35.219223414745684	38.0	34.0	41.0	24.0	41.0
39	35.12886589589318	38.0	34.0	41.0	24.0	41.0
40	34.966045760507626	38.0	34.0	40.0	23.0	41.0
41	34.887549006997936	38.0	34.0	40.0	23.0	41.0
42	34.86650184118636	38.0	34.0	40.0	23.0	41.0
43	34.77408333690614	38.0	34.0	40.0	23.0	41.0
44	34.639491803981535	38.0	34.0	40.0	23.0	41.0
45	34.53221690271009	38.0	34.0	40.0	23.0	41.0
46	34.47995121927982	38.0	34.0	40.0	23.0	41.0
47	34.40886513369443	37.0	34.0	40.0	23.0	41.0
48	34.27203311753581	37.0	33.0	40.0	22.0	41.0
49	34.172772164501545	37.0	33.0	40.0	22.0	41.0
50	34.08158480175687	37.0	33.0	40.0	21.0	41.0
51	33.75358914628976	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	9.0
12	25.0
13	29.0
14	68.0
15	180.0
16	419.0
17	1020.0
18	2222.0
19	4539.0
20	7745.0
21	12762.0
22	19701.0
23	29952.0
24	45221.0
25	69063.0
26	93249.0
27	104266.0
28	105532.0
29	108213.0
30	119265.0
31	139556.0
32	169600.0
33	216030.0
34	339687.0
35	503563.0
36	389612.0
37	481676.0
38	750151.0
39	1534156.0
40	460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.670268055773892	23.402188463169125	28.302688179726466	17.624855301330513
2	33.4497401378627	24.468542628347123	29.415174424420847	12.666542809369327
3	27.707525283561758	24.72532738818306	35.091497196537716	12.475650131717472
4	24.136452631729384	27.298167388373614	34.275391936889946	14.289988043007066
5	21.944121303931517	31.65731544071761	33.19743329569977	13.201129959651103
6	20.67212591523397	39.83753733582953	29.833697759612043	9.656638989324454
7	86.43680657777524	2.7588736607929727	9.612965000786017	1.191354760645773
8	87.41914738542009	2.1086609597035046	9.211324367970503	1.2608672869059019
9	82.88808540437026	5.049090363425893	10.214530366474687	1.848293865729162
10	37.14438426250125	38.76055049804925	15.38589646482691	8.709168774622594
11	26.609330265483354	25.16637369652104	32.14304565094155	16.081250387054055
12	28.524640456557055	23.12177554199477	31.750818172723765	16.602765828724415
13	23.11533496253317	27.32730243570139	31.799827552532168	17.757535049233276
14	17.037924151696608	31.57511230522249	32.50524249829696	18.88172104478394
15	16.158556395562098	28.689542156736646	38.479947027186675	16.671954420514577
16	20.5285276701966	26.431394966629984	36.92837713594292	16.111700227230504
17	20.409815214439856	26.229336077248846	31.81592900118617	21.54491970712513
18	20.59895483496015	27.29830077315536	33.873465479542105	18.22927891234238
19	23.13911556362216	28.674584006211916	30.271447558343933	17.91485287182199
20	24.093197852505014	28.578756568009567	30.517656810484045	16.810388769001378
21	22.30828081307552	27.085380551546073	33.68308728604843	16.92325134932998
22	22.109289773674607	24.40453698807635	33.13417079921303	20.35200243903601
23	19.096680148057107	28.564541561268868	32.988743277168815	19.35003501350521
24	18.7205350635245	28.115663660745334	35.57863747445443	17.58516380127573
25	19.719148814542752	29.601684459243806	32.684206765466676	17.994959960746765
26	18.503975342870348	31.87214115920903	29.987280808311777	19.636602689608846
27	17.619195975590586	30.978577451302648	32.62096332394876	18.78126324915801
28	16.79779343461049	30.054621068126274	34.587055006931244	18.560530490331985
29	18.023771073604582	27.779553065706295	34.55317527236696	19.643500588322162
30	19.082045932002345	28.852328755377073	33.51466041663689	18.550964895983686
31	21.651513202711524	28.81305646463636	31.092964429136956	18.442465903515163
32	22.45959632048552	28.64356251697083	30.895345347491176	18.001495815052472
33	21.702104144932093	29.209018716743124	30.041625579390146	19.047251558934637
34	19.287001176644324	29.528227554437663	31.65266602832521	19.532105240592802
35	18.801594900890343	29.245146937628324	31.98908150286539	19.964176658615944
36	21.9759431018631	30.17388611797884	29.29276530471277	18.55740547544529
37	19.482371771969188	31.340602803938662	30.681910641723714	18.49511478236844
38	19.70428593886213	31.181303264592536	29.90000905111019	19.214401745435143
39	19.555619072118294	30.328498134994923	29.70071313220814	20.415169660678643
40	21.556790952700805	28.828967363602153	30.36033898789533	19.253902695801713
41	18.219789537869367	28.794630309786157	31.589289202025544	21.39629095031893
42	20.18180345752409	29.825408848174774	29.208732892210804	20.784054802090328
43	20.073323520024392	29.964795945102633	29.37552103430371	20.58635950056927
44	20.024580909779484	31.10237758373468	29.157779905582633	19.715261600903204
45	18.815428808254612	31.55077911003768	28.33106102830139	21.302731053406315
46	19.947732220523154	31.90981283256875	28.789390193360298	19.353064753547798
47	19.64685426283471	30.729186019369376	30.340274105726493	19.28368561206942
48	19.834831530256906	29.935146413616682	30.67045860546211	19.559563450664307
49	19.591194698907675	29.296404803757643	30.541170642009536	20.57122985532515
50	18.417084685045186	30.9829219841939	30.90169065210867	19.69830267865224
51	18.036385462964287	31.217183770883057	30.039739137476833	20.70669162867582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6823.0
1	8019.0
2	9215.0
3	70599.0
4	131983.0
5	88848.0
6	45713.0
7	42494.0
8	39275.0
9	43005.0
10	46735.0
11	49617.5
12	52500.0
13	52744.5
14	52989.0
15	50508.0
16	48027.0
17	45457.5
18	42888.0
19	40897.5
20	38907.0
21	39305.5
22	39704.0
23	42798.5
24	45893.0
25	52224.0
26	72951.5
27	87348.0
28	96266.5
29	105185.0
30	120772.5
31	136360.0
32	150833.5
33	165307.0
34	187645.0
35	209983.0
36	221282.0
37	232581.0
38	248233.0
39	263885.0
40	288449.0
41	313013.0
42	341645.0
43	370277.0
44	365735.0
45	361193.0
46	360283.5
47	359374.0
48	364729.5
49	370085.0
50	354878.5
51	339672.0
52	315546.0
53	291420.0
54	262274.5
55	233129.0
56	221469.0
57	209809.0
58	192100.0
59	174391.0
60	153434.0
61	132477.0
62	110235.5
63	87994.0
64	70608.5
65	53223.0
66	44446.5
67	35670.0
68	28609.5
69	21549.0
70	17406.0
71	13263.0
72	11064.0
73	8865.0
74	6782.5
75	4262.0
76	3824.0
77	2820.0
78	1816.0
79	1517.0
80	1218.0
81	829.0
82	440.0
83	380.5
84	321.0
85	246.0
86	171.0
87	134.0
88	97.0
89	80.5
90	64.0
91	50.5
92	37.0
93	28.0
94	19.0
95	13.5
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5247975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.431492435046362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62705265461338	14.86076911063591
2	7.343452789200969	2.707015890625555
3	2.4328012436530275	1.3452047315508648
4	1.3011555356404707	0.9592895364790414
5	0.8553719606167199	0.7882890910628924
6	0.6735065094979884	0.7448238080859994
7	0.5376302636299255	0.6936529695862867
8	0.4590462922381783	0.6768726610179252
9	0.36019411031836107	0.5975023517533015
>10	3.129062461019362	12.447304899961983
>50	0.8997700593777987	11.94902864160955
>100	1.3694222919637133	48.51111802786863
>500	0.008807650648518454	1.0588119072935516
>1k	0.002411618629950207	0.66318997956943
>5k	2.097059678217571E-4	0.22452911710682008
>10k+	1.0485298391087854E-4	1.7725972757922746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91721	1.747740795259124	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6535	0.12452422124724298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.71649064639218E-5	0.0	0.0	0.02061747626465446	0.0
2	5.71649064639218E-5	0.0	0.0	0.06255746264035175	0.0
3	5.71649064639218E-5	0.0	0.0	0.10156298381756773	0.0
4	5.71649064639218E-5	0.0	0.0	0.16444438092788172	0.0
5	7.621987528522906E-5	0.0	0.0	0.3124633787317965	0.0
6	7.621987528522906E-5	0.0	0.0	0.5757268279669777	0.0
7	7.621987528522906E-5	0.0	0.0	0.7610363997541909	0.0
8	7.621987528522906E-5	0.0	0.0	1.0634387549483373	0.0
9	7.621987528522906E-5	0.0	0.0	1.2297124128830643	0.0
10	7.621987528522906E-5	0.0	0.0	1.4188139234657178	0.0
11	7.621987528522906E-5	0.0	0.0	1.6073437849837318	0.0
12	7.621987528522906E-5	0.0	0.0	1.7402140825747074	0.0
13	7.621987528522906E-5	0.0	0.0	1.809517004177802	0.0
14	7.621987528522906E-5	0.0	0.0	1.8530957178721317	0.0
15	7.621987528522906E-5	0.0	0.0	1.8889190592561893	0.0
16	7.621987528522906E-5	0.0	0.0	1.9469795492547126	1.9054968821307265E-5
17	7.621987528522906E-5	0.0	0.0	2.022532500631196	1.9054968821307265E-5
18	7.621987528522906E-5	0.0	0.0	2.1167783764213817	1.9054968821307265E-5
19	7.621987528522906E-5	0.0	0.0	2.1794120589370185	1.9054968821307265E-5
20	7.621987528522906E-5	0.0	0.0	2.244808711931745	1.9054968821307265E-5
21	7.621987528522906E-5	0.0	0.0	2.32548744992116	1.9054968821307265E-5
22	7.621987528522906E-5	0.0	0.0	2.427050433738728	1.9054968821307265E-5
23	7.621987528522906E-5	0.0	0.0	2.5266317007988794	1.9054968821307265E-5
24	7.621987528522906E-5	0.0	0.0	2.6078439779152913	1.9054968821307265E-5
25	7.621987528522906E-5	0.0	0.0	2.677890043302417	1.9054968821307265E-5
26	7.621987528522906E-5	0.0	0.0	2.7451540832416312	1.9054968821307265E-5
27	7.621987528522906E-5	0.0	0.0	2.819678066301764	1.9054968821307265E-5
28	7.621987528522906E-5	0.0	0.0	2.899480275725399	1.9054968821307265E-5
29	7.621987528522906E-5	0.0	0.0	2.996794001495815	1.9054968821307265E-5
30	7.621987528522906E-5	0.0	0.0	3.1259866901042783	3.810993764261453E-5
31	7.621987528522906E-5	0.0	0.0	3.2256632320085368	3.810993764261453E-5
32	7.621987528522906E-5	0.0	0.0	3.328312349048919	3.810993764261453E-5
33	7.621987528522906E-5	0.0	0.0	3.4266550431356855	3.810993764261453E-5
34	7.621987528522906E-5	0.0	0.0	3.5294565999266383	3.810993764261453E-5
35	7.621987528522906E-5	0.0	0.0	3.6650136481214184	3.810993764261453E-5
36	9.527484410653633E-5	0.0	0.0	3.7796102306127604	3.810993764261453E-5
37	9.527484410653633E-5	0.0	0.0	3.8971603332714047	3.810993764261453E-5
38	9.527484410653633E-5	0.0	0.0	4.041387392279879	3.810993764261453E-5
39	9.527484410653633E-5	0.0	0.0	4.290169065210867	3.810993764261453E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	36560	0.0	43.522976	1
ATACCCG	80	0.0	42.187504	41
CGCATCG	430	0.0	41.337208	21
CGGTCTA	825	0.0	40.363636	31
CTAAGCG	185	0.0	40.135136	1
CGACGGT	835	0.0	39.880238	28
CGTAAGG	565	0.0	39.82301	2
TAAGGGA	8085	0.0	38.988873	4
ACGGGTA	500	0.0	38.699997	5
GTAGGGA	8635	0.0	38.563984	4
TACGGGA	2285	0.0	38.402626	4
TAGGGAC	7000	0.0	38.282143	5
AGGGACC	5595	0.0	38.203754	6
GACCGAT	2475	0.0	38.0	9
GCGTAAT	30	1.14047674E-4	37.500004	44
TCACGAC	900	0.0	37.5	25
CTACGCA	90	0.0	37.5	23
GGACCGA	2815	0.0	37.48668	8
ACGGTCT	890	0.0	37.41573	30
CTCACGA	910	0.0	37.335163	24
>>END_MODULE