FastQCFastQC Report
Thu 26 May 2016
SRR1547485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547485_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4304664
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199980.4645658755247796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGT406.8230293E-945.038
CGAATCT453.8562575E-1045.037
TCGTAAG2650.042.452831
CGTACCG800.042.187514
CGTAAGG7050.041.4893652
CACGACG5150.040.63106526
TACGTCA403.461173E-739.37533
CGTTTTT109100.039.1223641
CGAATAC356.2522868E-638.57142644
CCGCGTA356.2522868E-638.57142624
CGGTCTA5200.038.50961331
TTGATCC19700.038.48984517
GGGCGAT57150.038.0708667
CGACGGT5500.038.04545628
AGGGCGA32350.037.905726
TACGCGG3850.037.40262
TACGGGA12650.037.1739164
TAGGGAC39350.037.0520945
TATGGGT10800.036.8754
CGTTAGG6050.036.8181842
Produced by FastQC (version 0.11.5)