##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547485_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4304664
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.583301507388267	31.0	31.0	33.0	30.0	34.0
2	32.017559094043115	31.0	31.0	34.0	30.0	34.0
3	32.07912998552268	33.0	31.0	34.0	30.0	34.0
4	35.81284439389462	37.0	35.0	37.0	35.0	37.0
5	35.75237347212233	37.0	35.0	37.0	35.0	37.0
6	35.808418496774664	37.0	35.0	37.0	35.0	37.0
7	36.11321278501644	37.0	35.0	37.0	35.0	37.0
8	36.116429760836155	37.0	35.0	37.0	35.0	37.0
9	37.958197201918665	39.0	38.0	39.0	35.0	39.0
10	37.424193154215985	39.0	37.0	39.0	35.0	39.0
11	37.11116152154965	39.0	37.0	39.0	33.0	39.0
12	36.57161000254607	39.0	35.0	39.0	33.0	39.0
13	36.32967985422324	39.0	35.0	39.0	32.0	39.0
14	37.36483985741977	40.0	35.0	41.0	32.0	41.0
15	37.515744550561905	40.0	35.0	41.0	32.0	41.0
16	37.61980935097373	40.0	35.0	41.0	33.0	41.0
17	37.55278391066062	39.0	35.0	41.0	33.0	41.0
18	37.45247875327784	39.0	35.0	41.0	33.0	41.0
19	37.42431883185308	39.0	35.0	41.0	32.0	41.0
20	37.30247447884434	39.0	35.0	41.0	32.0	41.0
21	37.17331224922549	39.0	35.0	41.0	32.0	41.0
22	37.11819970153304	39.0	35.0	41.0	32.0	41.0
23	37.02478706816606	39.0	35.0	41.0	32.0	41.0
24	36.882890279009	39.0	35.0	41.0	32.0	41.0
25	36.74755056376061	38.0	35.0	41.0	31.0	41.0
26	36.729137047630196	38.0	35.0	41.0	31.0	41.0
27	36.69476293620129	38.0	35.0	41.0	31.0	41.0
28	36.65277661624694	38.0	35.0	41.0	31.0	41.0
29	36.5788389058937	38.0	35.0	41.0	31.0	41.0
30	36.50749466160425	38.0	35.0	41.0	31.0	41.0
31	36.42451745362704	38.0	35.0	41.0	30.0	41.0
32	36.29944148950998	38.0	35.0	41.0	30.0	41.0
33	36.20106958406045	38.0	35.0	41.0	30.0	41.0
34	36.042397269566216	38.0	35.0	41.0	30.0	41.0
35	35.86259926442575	38.0	35.0	41.0	29.0	41.0
36	35.7169335399929	38.0	35.0	41.0	28.0	41.0
37	35.66146579616899	38.0	35.0	41.0	28.0	41.0
38	35.50497204892182	38.0	34.0	40.0	27.0	41.0
39	35.40407567234051	38.0	34.0	40.0	27.0	41.0
40	35.22878974990847	38.0	34.0	40.0	26.0	41.0
41	35.133330731504245	38.0	34.0	40.0	26.0	41.0
42	35.104262260654956	38.0	34.0	40.0	26.0	41.0
43	35.005502868516565	38.0	34.0	40.0	25.0	41.0
44	34.86750882298828	37.0	34.0	40.0	25.0	41.0
45	34.75333266429157	37.0	34.0	40.0	24.0	41.0
46	34.7074329146247	37.0	34.0	40.0	24.0	41.0
47	34.60985108245382	37.0	34.0	40.0	24.0	41.0
48	34.459377549560195	36.0	34.0	40.0	23.0	41.0
49	34.35918831295544	36.0	34.0	40.0	24.0	41.0
50	34.24097746072632	36.0	33.0	40.0	24.0	41.0
51	33.86232072932986	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	13.0
11	20.0
12	29.0
13	57.0
14	68.0
15	174.0
16	426.0
17	1063.0
18	2108.0
19	3927.0
20	6948.0
21	10720.0
22	16111.0
23	23471.0
24	32385.0
25	45413.0
26	59480.0
27	69772.0
28	76308.0
29	84827.0
30	98398.0
31	119473.0
32	147220.0
33	191542.0
34	328871.0
35	499148.0
36	299077.0
37	394720.0
38	613411.0
39	1179095.0
40	380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.828610084317845	22.907200190305215	29.334902793806904	13.929286931570037
2	31.09815307303892	25.652083414640494	29.789874424577622	13.459889087742969
3	29.748082544886195	25.379634740365336	30.880226656482364	13.992056058266103
4	26.573688445834563	27.441026756095248	30.77434150493511	15.210943293135074
5	23.621890117323908	32.51614992482572	29.511548404242465	14.3504115536079
6	22.459406820137414	40.799212203321794	26.410725668716534	10.330655307824257
7	88.4198395043144	3.958984952135637	6.144730459798953	1.47644508375102
8	89.55741958025062	2.875810980833812	5.868820423614944	1.6979490153006136
9	84.74698605977144	5.49109059383032	7.339527545006998	2.422395801391235
10	45.13441699514759	29.047679447222823	14.74800356078895	11.069899996840636
11	36.425723354947095	23.725034056084287	23.487686843851225	16.36155574511739
12	33.67008435501586	21.8509272733017	26.99532414144286	17.483664230239572
13	24.643735260173617	28.915264931246664	28.331549221960177	18.109450586619534
14	19.587243046147158	32.41590981317009	30.377469646876037	17.619377493806716
15	18.61264897794578	25.767028506754535	38.6056379777841	17.01468453751559
16	22.766585266585267	23.140505275208472	35.738677861965535	18.354231596240727
17	22.999309586067575	22.63491413034792	28.64572008407625	25.720056199508257
18	24.193990518191434	24.515293179676743	32.05181635546933	19.238899946662507
19	27.966503308969067	25.455970547294747	26.80027988247166	19.777246261264526
20	28.493420160086824	25.983979237403894	26.40566139424587	19.11693920826341
21	25.494812138647756	25.634358453993155	30.554998020751444	18.31583138660764
22	25.464124493804857	22.725815534034712	28.751396160071955	23.05866381208847
23	22.55095868109567	26.175864132485138	29.02784050044324	22.245336685975957
24	21.46857455076633	25.379379203580115	33.51522906317427	19.636817182479284
25	21.958113339391875	26.70837956226084	31.05494412572038	20.278562972626897
26	20.646907633209004	29.795612386936586	27.289423750610965	22.268056229243445
27	20.546644290936527	29.418300708255046	29.96598573082591	20.069069269982514
28	18.82537173633064	27.909402452781446	33.300415549274	19.964810261613913
29	20.51978969787189	25.920350577884825	32.961132390356134	20.59872733388715
30	22.08973801439555	26.750519901204832	31.367697920209338	19.792044164190283
31	26.268461371201095	26.552897043764624	27.21329701923309	19.96534456580119
32	27.062019242384537	26.170056478275654	27.43308188513668	19.334842394203125
33	26.150078147795043	26.73121525861252	26.93229947796158	20.18640711563086
34	22.018118022684234	26.832059366305945	29.482951514914983	21.666871096094837
35	22.18709752956328	26.902262290390144	29.865699157936596	21.044941022109974
36	27.923294361650523	25.80055028685166	26.637131260418933	19.639024091078884
37	23.08930499569769	28.731022909105103	28.0269261433645	20.15274595183271
38	22.96125783568706	30.107320803667836	25.469560458144937	21.46186090250017
39	22.443517078220275	28.076082128593544	27.266216364389877	22.2141844287963
40	24.066942274704832	25.771395862720066	27.655352427041922	22.506309435533183
41	20.63427017764917	25.20621818567024	28.66393288767718	25.495578749003407
42	22.86956659102778	26.55861177550675	26.91585220124033	23.65596943222514
43	22.502035002035	25.677776476863233	27.93086754273969	23.889320978362075
44	21.600756760574114	27.767602767602767	28.902766859387864	21.728873612435258
45	21.501283259274125	30.004804091562082	26.745037475631083	21.74887517353271
46	22.80749438283685	28.485916670848177	27.168531620586418	21.53805732572856
47	22.740520514493117	27.52728203641446	28.26411074127969	21.468086707812738
48	23.070255889890593	26.4527266239595	29.23203762244858	21.244979863701325
49	22.658400283971062	26.20276518678345	29.227809650184078	21.911024879061408
50	21.969658955960327	28.498623818258523	27.982091052867307	21.549626172913843
51	21.54495681892942	29.11827729179327	27.054585444996405	22.2821804442809
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4822.0
1	7328.5
2	9835.0
3	23402.0
4	36969.0
5	27316.0
6	17663.0
7	17371.0
8	17079.0
9	17903.0
10	18727.0
11	18551.0
12	18375.0
13	18176.0
14	17977.0
15	17304.5
16	16632.0
17	15551.0
18	14470.0
19	15459.0
20	16448.0
21	17915.5
22	19383.0
23	21176.5
24	22970.0
25	28239.5
26	37890.5
27	42272.0
28	50282.5
29	58293.0
30	68204.5
31	78116.0
32	90716.5
33	103317.0
34	112275.0
35	121233.0
36	135495.5
37	149758.0
38	167484.5
39	185211.0
40	211911.0
41	238611.0
42	278340.5
43	318070.0
44	326081.0
45	334092.0
46	328729.0
47	323366.0
48	314716.0
49	306066.0
50	296467.0
51	286868.0
52	278861.0
53	270854.0
54	250961.5
55	231069.0
56	220296.5
57	209524.0
58	199036.0
59	188548.0
60	175599.5
61	162651.0
62	145210.5
63	127770.0
64	111628.0
65	95486.0
66	85475.0
67	75464.0
68	61475.5
69	47487.0
70	42225.0
71	36963.0
72	28933.5
73	20904.0
74	16377.0
75	9602.5
76	7355.0
77	5785.0
78	4215.0
79	3181.0
80	2147.0
81	1734.0
82	1321.0
83	906.5
84	492.0
85	334.0
86	176.0
87	144.5
88	113.0
89	86.5
90	60.0
91	44.0
92	28.0
93	30.5
94	33.0
95	23.0
96	13.0
97	10.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4304664.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.101010652029252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.73500979027311	13.321172739289318
2	7.836587538790562	2.5235395887525307
3	2.3349711999226765	1.127861884864096
4	1.0321517023935196	0.6647474221899273
5	0.6220792608749746	0.5008052402877224
6	0.41747510271429666	0.4033062645455938
7	0.3054266764583363	0.34423747197486954
8	0.22593912736496688	0.29102786371308204
9	0.1839596844506128	0.2665743154994931
>10	1.7668727578802716	6.5018009005396715
>50	0.7140520013483256	8.474168210846496
>100	1.8061557460290663	62.67850239155529
>500	0.016414236837811437	1.6047761341531959
>1k	0.0027599159284815693	0.8297638663191297
>5k	0.0	0.0
>10k+	1.452587330779773E-4	0.4677157054695855
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19998	0.4645658755247796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.646123367584555E-5	0.0	0.0	0.01324145159761598	0.0
2	4.646123367584555E-5	0.0	0.0	0.05484748635433567	0.0
3	4.646123367584555E-5	0.0	0.0	0.08725419684323794	0.0
4	4.646123367584555E-5	0.0	0.0	0.16561106743755147	0.0
5	4.646123367584555E-5	0.0	0.0	0.2932400763450992	0.0
6	4.646123367584555E-5	0.0	0.0	0.4399646522934194	0.0
7	4.646123367584555E-5	0.0	0.0	0.5199476660663875	0.0
8	4.646123367584555E-5	0.0	0.0	0.6816792204920059	0.0
9	4.646123367584555E-5	0.0	0.0	0.7401739136898954	0.0
10	4.646123367584555E-5	0.0	0.0	0.8577440655066226	0.0
11	4.646123367584555E-5	0.0	0.0	1.0389428768424203	0.0
12	6.969185051376832E-5	0.0	0.0	1.1785124228046602	0.0
13	6.969185051376832E-5	0.0	0.0	1.236635426133143	0.0
14	6.969185051376832E-5	0.0	0.0	1.2604932696256896	0.0
15	6.969185051376832E-5	0.0	0.0	1.2978248708842317	0.0
16	6.969185051376832E-5	0.0	0.0	1.3805026362103987	0.0
17	6.969185051376832E-5	0.0	0.0	1.4735644872631175	0.0
18	6.969185051376832E-5	0.0	0.0	1.5926446291743095	0.0
19	6.969185051376832E-5	0.0	0.0	1.6582711217414414	0.0
20	6.969185051376832E-5	0.0	0.0	1.728148817189913	0.0
21	9.29224673516911E-5	0.0	0.0	1.8231852706738552	0.0
22	9.29224673516911E-5	0.0	0.0	1.9208467838604826	0.0
23	9.29224673516911E-5	0.0	0.0	2.0261047087531106	0.0
24	9.29224673516911E-5	0.0	0.0	2.1052514203199135	0.0
25	1.1615308418961387E-4	0.0	0.0	2.172202058046807	0.0
26	1.1615308418961387E-4	0.0	0.0	2.2423585208973336	0.0
27	1.1615308418961387E-4	0.0	0.0	2.3285441093660273	0.0
28	1.1615308418961387E-4	0.0	0.0	2.4065757513246098	0.0
29	1.1615308418961387E-4	0.0	0.0	2.5023091233136894	0.0
30	1.1615308418961387E-4	0.0	0.0	2.6091467301512963	0.0
31	1.1615308418961387E-4	0.0	0.0	2.70192981380196	0.0
32	1.3938370102753664E-4	0.0	0.0	2.7910889212259073	0.0
33	1.3938370102753664E-4	0.0	0.0	2.88008541433199	0.0
34	1.3938370102753664E-4	0.0	0.0	2.975470327068501	0.0
35	1.3938370102753664E-4	0.0	0.0	3.0907406478182735	0.0
36	1.6261431786545943E-4	0.0	0.0	3.1839418825720194	0.0
37	1.6261431786545943E-4	0.0	0.0	3.2904077995402194	0.0
38	1.6261431786545943E-4	0.0	0.0	3.4179671165972536	0.0
39	1.6261431786545943E-4	0.0	0.0	3.6499248257239123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGT	40	6.8230293E-9	45.0	38
CGAATCT	45	3.8562575E-10	45.0	37
TCGTAAG	265	0.0	42.45283	1
CGTACCG	80	0.0	42.1875	14
CGTAAGG	705	0.0	41.489365	2
CACGACG	515	0.0	40.631065	26
TACGTCA	40	3.461173E-7	39.375	33
CGTTTTT	10910	0.0	39.122364	1
CGAATAC	35	6.2522868E-6	38.571426	44
CCGCGTA	35	6.2522868E-6	38.571426	24
CGGTCTA	520	0.0	38.509613	31
TTGATCC	1970	0.0	38.489845	17
GGGCGAT	5715	0.0	38.070866	7
CGACGGT	550	0.0	38.045456	28
AGGGCGA	3235	0.0	37.90572	6
TACGCGG	385	0.0	37.4026	2
TACGGGA	1265	0.0	37.173916	4
TAGGGAC	3935	0.0	37.052094	5
TATGGGT	1080	0.0	36.875	4
CGTTAGG	605	0.0	36.818184	2
>>END_MODULE