FastQCFastQC Report
Thu 26 May 2016
SRR1547484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547484_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2702352
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447251.6550397579589926No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34650.12822163803975203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT224200.043.043041
TACGGGA11150.039.7533654
CGTTAGG4000.039.3752
ACGGGAT11800.038.516955
ACGGGTA1700.038.382355
TCGTAAG1650.038.1818161
GGGCGAT37250.038.1744967
TAGGGAC29000.038.1724175
AATACGG1550.037.7419362
TAGGGAT41200.037.5182085
AGGGATT48500.037.1597946
ATAGGGA34900.037.134674
CGGTTTT15400.037.110391
TAAGGGA40150.037.098384
CTACGGG4250.037.0588263
GTAGGGA35950.036.8011134
TTAGGGA35500.036.697184
AGGGCGA20200.036.4232676
CGGGATA6000.036.3750046
ATATGCG1250.036.0000041
Produced by FastQC (version 0.11.5)