##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547484_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2702352
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.499106334037904	31.0	31.0	33.0	30.0	34.0
2	31.905701773862177	31.0	31.0	34.0	30.0	34.0
3	31.714146417639153	31.0	31.0	34.0	30.0	34.0
4	35.619868544142285	37.0	35.0	37.0	33.0	37.0
5	35.65256006619419	37.0	35.0	37.0	33.0	37.0
6	35.74434492619763	37.0	35.0	37.0	33.0	37.0
7	36.0622602088847	37.0	35.0	37.0	35.0	37.0
8	36.07401737449452	37.0	35.0	37.0	35.0	37.0
9	37.922026072103115	39.0	38.0	39.0	35.0	39.0
10	37.4104683623747	39.0	37.0	39.0	35.0	39.0
11	37.10603503910667	39.0	37.0	39.0	33.0	39.0
12	36.84947075732547	39.0	35.0	39.0	33.0	39.0
13	36.75156567316175	39.0	35.0	39.0	33.0	39.0
14	37.91806063754833	40.0	37.0	41.0	33.0	41.0
15	37.988482995553504	40.0	37.0	41.0	33.0	41.0
16	38.023380743885326	40.0	37.0	41.0	33.0	41.0
17	37.91887548328271	40.0	36.0	41.0	33.0	41.0
18	37.706315461494285	39.0	36.0	41.0	33.0	41.0
19	37.57748990509008	39.0	36.0	41.0	33.0	41.0
20	37.376957183964194	39.0	35.0	41.0	32.0	41.0
21	37.26863561815781	39.0	35.0	41.0	32.0	41.0
22	37.220453146000224	39.0	35.0	41.0	32.0	41.0
23	37.09964616008573	39.0	35.0	41.0	32.0	41.0
24	36.9565175077118	39.0	35.0	41.0	32.0	41.0
25	36.82818892579501	38.0	35.0	41.0	31.0	41.0
26	36.78676316038769	39.0	35.0	41.0	31.0	41.0
27	36.733927334410915	39.0	35.0	41.0	31.0	41.0
28	36.67863734998253	38.0	35.0	41.0	31.0	41.0
29	36.57812860796817	38.0	35.0	41.0	31.0	41.0
30	36.46880717241869	38.0	35.0	41.0	31.0	41.0
31	36.33817911212159	38.0	35.0	41.0	30.0	41.0
32	36.093649531963266	38.0	35.0	41.0	30.0	41.0
33	35.84746065649479	38.0	35.0	41.0	28.0	41.0
34	35.53849424501323	38.0	35.0	41.0	26.0	41.0
35	35.20856979401647	38.0	34.0	41.0	24.0	41.0
36	35.04484389894433	38.0	34.0	41.0	23.0	41.0
37	34.98281645026259	38.0	34.0	41.0	23.0	41.0
38	34.79722848836865	38.0	34.0	40.0	23.0	41.0
39	34.67433258139576	38.0	34.0	40.0	22.0	41.0
40	34.51574443299763	38.0	34.0	40.0	21.0	41.0
41	34.40187140683375	38.0	34.0	40.0	21.0	41.0
42	34.35624707662066	38.0	33.0	40.0	21.0	41.0
43	34.234501648933964	38.0	33.0	40.0	20.0	41.0
44	34.07869330124277	38.0	33.0	40.0	19.0	41.0
45	33.95802323309473	37.0	33.0	40.0	20.0	41.0
46	33.88978711877653	37.0	33.0	40.0	20.0	41.0
47	33.81167183253699	37.0	33.0	40.0	20.0	41.0
48	33.654892848896075	37.0	33.0	40.0	19.0	41.0
49	33.55128680497582	36.0	33.0	40.0	18.0	41.0
50	33.4495946494017	36.0	33.0	40.0	18.0	41.0
51	33.08681659532141	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	11.0
12	10.0
13	23.0
14	37.0
15	100.0
16	273.0
17	666.0
18	1446.0
19	2787.0
20	4849.0
21	7670.0
22	11669.0
23	17107.0
24	25912.0
25	39650.0
26	55913.0
27	63606.0
28	63064.0
29	62383.0
30	66080.0
31	74817.0
32	88836.0
33	111626.0
34	172517.0
35	230265.0
36	199310.0
37	261992.0
38	406244.0
39	733307.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.153176196143214	22.7350841045134	25.868576706513437	19.243162992829948
2	35.89906126218938	23.666457959584836	27.56128735264688	12.873193425578902
3	27.90280466793371	24.301312338289016	34.81992723375785	12.97595576001942
4	24.210280526001053	26.82896232615144	34.908627743536	14.052129404311502
5	22.091163549382166	30.951704293149078	33.293664185864756	13.663467971603996
6	21.13629164520388	39.47587138907145	29.786090043043984	9.601746922680688
7	84.49091754146018	3.0241064080475084	11.13448581087882	1.3504902396134923
8	85.03403701664328	2.480172827226061	11.040826657667099	1.4449634984635606
9	80.56974072955707	5.001902046809594	12.199224971432294	2.2291322522010453
10	37.31634516894912	34.337347614226424	18.132167830097636	10.21413938672682
11	27.26243657376981	24.668436976382054	31.076484484626725	16.99264196522141
12	26.1011888902704	22.46517108059942	33.507773968750186	17.925866060379995
13	23.42370646015027	24.03062221353843	33.845923847078396	18.699747479232904
14	19.192503419243682	26.88872508096651	34.75724109960508	19.161530400184727
15	18.560350391066745	25.912575415785955	37.39387022860086	18.13320396454644
16	23.287158741718326	24.59723973782838	34.886572881697134	17.229028638756162
17	22.61770487338437	24.54065939596322	32.78281289780162	20.05882283285079
18	23.181843075957538	24.94401173496273	33.4403512199743	18.43379396910543
19	24.531334185923964	25.674190482957073	31.157784034056256	18.636691297062708
20	25.43765579021534	26.023071753790774	31.301436674422874	17.237835781571018
21	24.88361989851803	24.52600549447296	33.41141346501122	17.178961141997785
22	24.21986476965251	22.147336838428156	33.16969810002546	20.463100291893877
23	21.577129848369125	25.087997418545033	33.46259110582189	19.872281627263956
24	21.025573278388602	25.68455182744513	34.85867126118285	18.43120363298342
25	22.41018194520921	26.47045980686454	32.301972503952115	18.81738574397414
26	21.4073518179719	27.37378402221472	30.635794300668458	20.583069859144924
27	20.26864005873402	26.842950141210327	33.06334629981586	19.825063500239793
28	19.770925475289676	27.053655482335387	33.74186634457687	19.433552697798063
29	20.57770416289218	25.773400356430248	33.779944285570494	19.868951195107076
30	21.381337442346517	25.482875657945375	33.99342498682629	19.142361912881817
31	23.336634161648814	25.48269063393666	31.633073707644304	19.54760149677022
32	24.06662788563444	25.400836012480983	31.485202519878978	19.047333582005603
33	22.943273119119937	25.577570945605903	30.942897150334225	20.536258784939935
34	20.986459202946175	26.920512205663805	32.114580187925185	19.97844840346483
35	21.129112713665727	27.37811358401866	31.830975387366266	19.661798314949348
36	23.37978176048124	28.432232366471872	28.997702741907787	19.190283131139097
37	22.040985038218558	28.234404696353398	29.552293705631243	20.172316559796798
38	22.045425614427728	28.828516788338455	29.03193218352013	20.094125413713684
39	21.441840293196446	28.367955025844154	28.580251573444173	21.60995310751523
40	23.131997608009616	27.429513253639794	29.346806041551954	20.09168309679864
41	20.630695038988257	27.51062777906061	30.225818102156936	21.632859079794194
42	22.095826154401795	27.705088012220465	28.778042238760904	21.421043594616837
43	21.635153377502263	27.16252360906351	29.5137717070167	21.68855130641752
44	21.63171193094016	28.0716945830891	29.470106040959877	20.826487445010862
45	21.462414962965596	28.074025885598914	28.862339177131624	21.601219974303866
46	21.644034529920603	28.206207037425173	29.078558233716407	21.071200198937813
47	21.296337412742677	28.33650094436254	30.22933355832253	20.137828084572256
48	21.697987530862004	27.836788101624066	30.122278666879815	20.342945700634115
49	22.18567381303398	27.254184502981104	29.970706998940184	20.58943468504473
50	20.84761718680616	28.10144644369053	30.541025003404442	20.509911366098866
51	20.234299602716447	28.314779125739353	30.143334399071627	21.307586872472573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2643.0
1	3733.5
2	4824.0
3	39266.5
4	73709.0
5	51905.0
6	30101.0
7	27433.0
8	24765.0
9	24991.5
10	25218.0
11	24732.5
12	24247.0
13	23428.0
14	22609.0
15	21338.0
16	20067.0
17	19164.5
18	18262.0
19	17651.5
20	17041.0
21	17286.5
22	17532.0
23	18381.0
24	19230.0
25	21625.0
26	27581.5
27	31143.0
28	36194.0
29	41245.0
30	48213.5
31	55182.0
32	63796.0
33	72410.0
34	79537.0
35	86664.0
36	93708.0
37	100752.0
38	108967.5
39	117183.0
40	127324.0
41	137465.0
42	147645.0
43	157825.0
44	161535.5
45	165246.0
46	168875.0
47	172504.0
48	173533.0
49	174562.0
50	169799.0
51	165036.0
52	159675.0
53	154314.0
54	146944.0
55	139574.0
56	134275.0
57	128976.0
58	122348.5
59	115721.0
60	107963.0
61	100205.0
62	88449.5
63	76694.0
64	67976.5
65	59259.0
66	50197.5
67	41136.0
68	35310.5
69	29485.0
70	25318.5
71	21152.0
72	17801.0
73	14450.0
74	11484.5
75	7064.0
76	5609.0
77	4295.5
78	2982.0
79	2188.0
80	1394.0
81	1043.5
82	693.0
83	526.5
84	360.0
85	272.0
86	184.0
87	127.0
88	70.0
89	57.5
90	45.0
91	33.5
92	22.0
93	18.0
94	14.0
95	10.5
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2702352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.838420931719924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6045558504962	17.9520393366452
2	5.5329989778099025	2.5272991933999727
3	2.2588521984531895	1.5476585199244468
4	1.4382693566144178	1.3139120391821628
5	1.0039862894280418	1.1464730743816602
6	0.7807181813953622	1.0698222273452491
7	0.6201825925133084	0.9914793771640988
8	0.5246083338129729	0.9584980761527131
9	0.42986850975793744	0.8835766174028638
>10	6.998632163694017	39.43461432517371
>50	1.6700455553672091	25.542335734715504
>100	0.13284347375459368	4.105544794932398
>500	0.003452179813316341	0.5243390764309426
>1k	8.219475745991288E-4	0.32325357640487007
>5k	0.0	0.0
>10k+	1.6438951491982575E-4	1.6791540307442294
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44725	1.6550397579589926	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3465	0.12822163803975203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.400960348614836E-5	0.0	0.0	0.014838925498972746	0.0
2	7.400960348614836E-5	0.0	0.0	0.04307358922893835	0.0
3	7.400960348614836E-5	0.0	0.0	0.07508274273669752	0.0
4	7.400960348614836E-5	0.0	0.0	0.12204183614865866	0.0
5	7.400960348614836E-5	0.0	0.0	0.22672842027981552	0.0
6	7.400960348614836E-5	0.0	0.0	0.44076419356175656	0.0
7	1.1101440522922254E-4	0.0	0.0	0.5779779984250757	0.0
8	1.1101440522922254E-4	0.0	0.0	0.798156568796367	0.0
9	1.1101440522922254E-4	0.0	0.0	0.9252310579820837	0.0
10	1.4801920697229672E-4	0.0	0.0	1.0641100789238411	0.0
11	1.4801920697229672E-4	0.0	0.0	1.1716090279874716	0.0
12	1.4801920697229672E-4	0.0	0.0	1.2465067467154538	0.0
13	1.4801920697229672E-4	0.0	0.0	1.292022652859435	0.0
14	1.4801920697229672E-4	0.0	0.0	1.3231066863236174	0.0
15	1.4801920697229672E-4	0.0	0.0	1.3476408698792754	0.0
16	1.4801920697229672E-4	0.0	0.0	1.3828694411386822	0.0
17	1.4801920697229672E-4	0.0	0.0	1.4298285345506432	0.0
18	1.4801920697229672E-4	0.0	0.0	1.4872229820541514	0.0
19	1.4801920697229672E-4	0.0	0.0	1.5251899086425456	0.0
20	1.4801920697229672E-4	0.0	0.0	1.5660062049651562	0.0
21	1.4801920697229672E-4	0.0	0.0	1.613298341592805	0.0
22	1.4801920697229672E-4	0.0	0.0	1.6682874769830134	0.0
23	1.4801920697229672E-4	0.0	0.0	1.727569169375418	0.0
24	1.4801920697229672E-4	0.0	0.0	1.7790428486000343	0.0
25	1.4801920697229672E-4	0.0	0.0	1.824188706726585	0.0
26	1.4801920697229672E-4	0.0	0.0	1.8695565936635938	0.0
27	1.4801920697229672E-4	0.0	0.0	1.9259519115200388	0.0
28	1.4801920697229672E-4	0.0	0.0	1.9792018212283227	0.0
29	1.4801920697229672E-4	0.0	0.0	2.0395936576730196	0.0
30	1.4801920697229672E-4	0.0	0.0	2.113085193934765	0.0
31	1.4801920697229672E-4	0.0	0.0	2.172403891128913	0.0
32	1.4801920697229672E-4	0.0	0.0	2.243416105673872	0.0
33	1.4801920697229672E-4	0.0	0.0	2.308063494319023	0.0
34	1.4801920697229672E-4	0.0	0.0	2.3733769693955487	0.0
35	1.4801920697229672E-4	0.0	0.0	2.450383961822886	0.0
36	1.4801920697229672E-4	0.0	0.0	2.517621686590052	0.0
37	1.4801920697229672E-4	0.0	0.0	2.592001338093631	0.0
38	1.4801920697229672E-4	0.0	0.0	2.6822560495449888	0.0
39	1.4801920697229672E-4	0.0	0.0	2.8376762168659004	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22420	0.0	43.04304	1
TACGGGA	1115	0.0	39.753365	4
CGTTAGG	400	0.0	39.375	2
ACGGGAT	1180	0.0	38.51695	5
ACGGGTA	170	0.0	38.38235	5
TCGTAAG	165	0.0	38.181816	1
GGGCGAT	3725	0.0	38.174496	7
TAGGGAC	2900	0.0	38.172417	5
AATACGG	155	0.0	37.741936	2
TAGGGAT	4120	0.0	37.518208	5
AGGGATT	4850	0.0	37.159794	6
ATAGGGA	3490	0.0	37.13467	4
CGGTTTT	1540	0.0	37.11039	1
TAAGGGA	4015	0.0	37.09838	4
CTACGGG	425	0.0	37.058826	3
GTAGGGA	3595	0.0	36.801113	4
TTAGGGA	3550	0.0	36.69718	4
AGGGCGA	2020	0.0	36.423267	6
CGGGATA	600	0.0	36.375004	6
ATATGCG	125	0.0	36.000004	1
>>END_MODULE