FastQCFastQC Report
Thu 26 May 2016
SRR1547483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547483_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences291856
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.376898196370813No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2930.10039197412422564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG253.8836304E-545.0000043
ATTAACG351.2077726E-745.01
TAACGAG207.0246286E-445.01
CGGGGTA453.8198777E-1045.06
TGCATAC207.0246286E-445.035
GCGATAT207.0246286E-445.09
CCTATCC207.0246286E-445.020
CGGGTAC302.159919E-644.9999966
CGAGGGT302.159919E-644.9999964
GCGATCA302.159919E-644.9999969
TAGGGTG950.042.631585
TACGGGA900.042.54
ACGGGAG1350.041.6666645
TTAACGG650.041.538462
CGTTTTT5850.041.1538471
AGGGCGA2300.041.0869566
CGAAAGG556.002665E-1140.9090922
TAGACGG556.002665E-1140.9090922
TATTGCG556.002665E-1140.9090921
CGAGGGC1050.040.7142874
Produced by FastQC (version 0.11.5)