##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547483_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	291856
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54301093690039	31.0	31.0	33.0	30.0	34.0
2	31.984464941615045	31.0	31.0	34.0	30.0	34.0
3	32.08494942711474	33.0	31.0	34.0	30.0	34.0
4	35.81749904062277	37.0	35.0	37.0	35.0	37.0
5	35.76858450742832	37.0	35.0	37.0	35.0	37.0
6	35.81975357710652	37.0	35.0	37.0	35.0	37.0
7	36.0870326462365	37.0	35.0	37.0	35.0	37.0
8	36.092891700016445	37.0	35.0	37.0	35.0	37.0
9	37.97892796447563	39.0	38.0	39.0	35.0	39.0
10	37.40269516473877	39.0	37.0	39.0	35.0	39.0
11	37.10144043637958	39.0	37.0	39.0	33.0	39.0
12	36.61902102406666	39.0	35.0	39.0	33.0	39.0
13	36.40646414670248	39.0	35.0	39.0	32.0	39.0
14	37.48632887451346	40.0	35.0	41.0	33.0	41.0
15	37.625585905377996	40.0	35.0	41.0	33.0	41.0
16	37.71681582698317	40.0	35.0	41.0	33.0	41.0
17	37.61059220985692	40.0	35.0	41.0	33.0	41.0
18	37.5281542952689	39.0	36.0	41.0	33.0	41.0
19	37.513989775779834	39.0	36.0	41.0	33.0	41.0
20	37.417836193191164	39.0	35.0	41.0	32.0	41.0
21	37.3018509127789	39.0	35.0	41.0	32.0	41.0
22	37.23825105531495	39.0	35.0	41.0	32.0	41.0
23	37.15177005098405	39.0	35.0	41.0	32.0	41.0
24	37.024364755221754	39.0	35.0	41.0	32.0	41.0
25	36.88944890630996	39.0	35.0	41.0	31.0	41.0
26	36.85704594046379	39.0	35.0	41.0	31.0	41.0
27	36.821901896825835	39.0	35.0	41.0	31.0	41.0
28	36.787124472342526	39.0	35.0	41.0	31.0	41.0
29	36.74523395098953	39.0	35.0	41.0	31.0	41.0
30	36.69099830053177	38.0	35.0	41.0	31.0	41.0
31	36.62876555561647	39.0	35.0	41.0	31.0	41.0
32	36.524834164793596	39.0	35.0	41.0	31.0	41.0
33	36.441464283756375	39.0	35.0	41.0	30.0	41.0
34	36.335045364837455	39.0	35.0	41.0	30.0	41.0
35	36.19030960473658	38.0	35.0	41.0	30.0	41.0
36	36.058319856367525	38.0	35.0	41.0	30.0	41.0
37	36.01639849788937	38.0	35.0	41.0	30.0	41.0
38	35.864899128337264	38.0	35.0	41.0	29.0	41.0
39	35.78191299819089	38.0	35.0	41.0	29.0	41.0
40	35.60712132010306	38.0	35.0	40.0	28.0	41.0
41	35.543463214736036	38.0	35.0	40.0	27.0	41.0
42	35.495275067156406	38.0	35.0	40.0	27.0	41.0
43	35.40163299709446	38.0	34.0	40.0	27.0	41.0
44	35.265254097911296	38.0	34.0	40.0	27.0	41.0
45	35.16024683405515	38.0	34.0	40.0	27.0	41.0
46	35.11925058933173	37.0	34.0	40.0	26.0	41.0
47	35.02891837070336	37.0	34.0	40.0	26.0	41.0
48	34.88024573762404	37.0	34.0	40.0	26.0	41.0
49	34.78248519817993	37.0	34.0	40.0	26.0	41.0
50	34.696651773477335	37.0	34.0	40.0	25.0	41.0
51	34.30234088043419	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	3.0
14	2.0
15	11.0
16	24.0
17	60.0
18	150.0
19	210.0
20	456.0
21	649.0
22	969.0
23	1324.0
24	1835.0
25	2605.0
26	3441.0
27	4173.0
28	4721.0
29	5513.0
30	6341.0
31	7707.0
32	9879.0
33	12898.0
34	21638.0
35	32562.0
36	20573.0
37	27131.0
38	43142.0
39	83807.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.95582753138534	24.265048517076913	29.937366372457653	13.841757579080094
2	29.072898963872596	26.043322734499203	31.148580121703855	13.735198179924346
3	28.721013102351844	25.92614165890028	31.932185735431172	13.420659503316704
4	25.442684063373715	28.19095718436489	31.443245984321035	14.923112767940355
5	23.263184584178497	32.39577051696727	29.7197933227345	14.621251576119732
6	22.27297023189518	41.92752590318513	25.881256510059757	9.91824735485993
7	90.6676580231347	3.1553231730716518	4.792431884216874	1.3845869195767775
8	91.75826434954224	2.3449920508744038	4.474124225645524	1.4226193739378323
9	87.7233978400307	4.412107340606326	5.39204265117044	2.4724521681925333
10	43.2295378542843	29.333301354092427	14.288210624417522	13.148950167205745
11	34.0883860533962	24.740625513952086	24.131420974727263	17.039567457924456
12	32.203209802094186	23.053149498382762	26.70906200317965	18.034578696343402
13	24.340085521627103	28.156351077243574	27.436475522175318	20.067087878954005
14	18.931253768981964	31.881133161559127	29.828065895510115	19.359547173948798
15	18.436146592840306	26.50211062989968	36.94116276519927	18.120580012060742
16	23.271750452277836	24.24380516419056	33.4181925333041	19.06625185022751
17	21.97933227344992	24.010128282440657	28.432514664766185	25.578024779343238
18	24.176306123567787	25.30631544323228	30.50168576284195	20.015692670357986
19	26.92526451400691	26.664862123787074	26.03989638725947	20.36997697494655
20	28.043281618332326	27.151403431829397	25.470780110739543	19.334534839098733
21	25.745915794090234	26.2447919521956	29.336727701332165	18.672564552381996
22	25.086001315717336	23.091524587467795	28.64529082835371	23.17718326846116
23	22.3695932240557	26.831725234362153	28.482881969190288	22.315799572391864
24	21.950208321912175	26.1002000986788	32.458130036730445	19.491461542678582
25	22.12735047420646	26.461679732470806	29.62077188750617	21.790197905816566
26	20.513883559015405	30.422194506880107	26.74435337974892	22.319568554355573
27	20.126363686201415	29.49742338687572	29.298009977523158	21.078202949399703
28	19.079614604462474	27.800010964311166	32.872375418014364	20.247999013211995
29	20.164053505838496	25.91928896442081	32.687695301792665	21.228962227948028
30	21.39034318293953	27.35561372731758	30.91867222191766	20.33537086782523
31	25.933336988103722	25.843909325146647	27.220958280796008	21.001795405953622
32	26.74161230195713	26.34998081245546	27.049983553533245	19.858423332054166
33	24.978756647113645	26.775875774354475	27.050668822981194	21.194698755550682
34	20.98877528644263	27.279891453319443	28.744654898306017	22.98667836193191
35	21.351282824406557	27.30490378816951	29.83354805109369	21.510265336330246
36	26.40685817663505	26.426045721177566	26.934173016830215	20.232923085357164
37	22.07698316978236	28.908776931089303	27.539265939367358	21.47497395976098
38	22.47238364124774	29.73863823255304	25.830889205635653	21.958088920563565
39	21.624705334137385	28.891645194890632	26.43529685872485	23.048352612247136
40	23.49549092703251	26.856737569212214	27.325119236883943	22.322652266871334
41	21.159064744257442	25.563976755660327	28.554149991776768	24.722808508305466
42	23.19705608245162	26.560015898251194	26.538087275916887	23.7048407433803
43	22.076297900334414	26.13788991831588	27.93329587193685	23.85251630941286
44	21.3804067759443	27.672208212269062	28.20363466915191	22.743750342634726
45	20.694452058549423	30.16556109862398	26.2797406940409	22.860246148785702
46	21.96734005811085	29.074612137492462	26.937941998793924	22.020105805602764
47	21.64149443561208	27.680774080368405	28.3341784989858	22.343552985033714
48	22.27468340551505	26.8762677484787	28.685379091058604	22.163669754947644
49	22.16709610218738	26.860506551175924	29.21851872156132	21.75387862507538
50	21.59489611315169	28.45067430513678	27.783907132284412	22.170522449427114
51	20.19523326572008	30.437955704182883	27.049640918809274	22.31717011128776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	406.0
1	499.5
2	593.0
3	1305.0
4	2017.0
5	1464.5
6	912.0
7	921.5
8	931.0
9	993.5
10	1056.0
11	1037.0
12	1018.0
13	1005.0
14	992.0
15	924.5
16	857.0
17	814.5
18	772.0
19	878.0
20	984.0
21	1203.5
22	1423.0
23	1474.0
24	1525.0
25	1757.5
26	2481.0
27	2972.0
28	3623.5
29	4275.0
30	4959.0
31	5643.0
32	6452.0
33	7261.0
34	7852.5
35	8444.0
36	9428.5
37	10413.0
38	11649.5
39	12886.0
40	14502.0
41	16118.0
42	18541.0
43	20964.0
44	21639.5
45	22315.0
46	22206.5
47	22098.0
48	22303.5
49	22509.0
50	21887.5
51	21266.0
52	20299.5
53	19333.0
54	18005.0
55	16677.0
56	15481.5
57	14286.0
58	13540.5
59	12795.0
60	11593.0
61	10391.0
62	9049.5
63	7708.0
64	6844.0
65	5980.0
66	5077.0
67	4174.0
68	3478.5
69	2783.0
70	2350.5
71	1918.0
72	1614.5
73	1311.0
74	1049.5
75	641.5
76	495.0
77	381.0
78	267.0
79	208.0
80	149.0
81	109.5
82	70.0
83	49.0
84	28.0
85	33.5
86	39.0
87	23.5
88	8.0
89	7.0
90	6.0
91	6.5
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	291856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.756304478921113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.63670809155576	20.024943807905267
2	5.886067654032671	3.385231072857848
3	3.251635349768251	2.805150485170769
4	2.334171363208503	2.684885697056082
5	2.0041226303811643	2.881558028616852
6	1.7217343643880993	2.9706430568499536
7	1.5358585437344359	3.091593114412587
8	1.3988347015859022	3.218025327558796
9	1.2344060910076613	3.194726166328601
>10	10.95714132519928	54.455621950550956
>50	0.03336232678399085	0.6218820240118414
>100	0.004766046683427264	0.2888410723096322
>500	0.0	0.0
>1k	0.001191511670856816	0.376898196370813
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1100	0.376898196370813	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	293	0.10039197412422564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02055808343840798	0.0
2	0.0	0.0	0.0	0.0681843100707198	0.0
3	0.0	0.0	0.0	0.10930047694753577	0.0
4	0.0	0.0	0.0	0.19119017597719423	0.0
5	0.0	0.0	0.0	0.346061071213201	0.0
6	0.0	0.0	0.0	0.5492434625294665	0.0
7	0.0	0.0	0.0	0.6451811852420372	0.0
8	0.0	0.0	0.0	0.8689216599967107	0.0
9	0.0	0.0	0.0	0.9597198618496793	0.0
10	0.0	0.0	0.0	1.134463571076147	0.0
11	0.0	0.0	0.0	1.3191436872978455	0.0
12	0.0	0.0	0.0	1.4479743435118688	0.0
13	0.0	0.0	0.0	1.508278054931199	0.0
14	0.0	0.0	0.0	1.5312345814374213	0.0
15	0.0	0.0	0.0	1.5675538621786087	0.0
16	0.0	0.0	0.0	1.6350529028013816	0.0
17	0.0	0.0	0.0	1.7148867934871992	0.0
18	0.0	0.0	0.0	1.8049997258922208	0.0
19	0.0	0.0	0.0	1.8553670303163203	0.0
20	0.0	0.0	0.0	1.9173839153555179	0.0
21	0.0	0.0	0.0	2.0023573269009374	0.0
22	0.0	0.0	0.0	2.1020640315772163	0.0
23	0.0	0.0	0.0	2.1949180417740255	0.0
24	0.0	0.0	0.0	2.272696124116002	0.0
25	0.0	0.0	0.0	2.3329998355353325	0.0
26	0.0	0.0	0.0	2.3953593552985035	0.0
27	0.0	0.0	0.0	2.4621731264733295	0.0
28	3.426347239734664E-4	0.0	0.0	2.5344690532317307	0.0
29	3.426347239734664E-4	0.0	0.0	2.621155638397018	0.0
30	3.426347239734664E-4	0.0	0.0	2.717778630557535	0.0
31	3.426347239734664E-4	0.0	0.0	2.8082341976865304	0.0
32	3.426347239734664E-4	0.0	0.0	2.8884107230963214	0.0
33	3.426347239734664E-4	0.0	0.0	2.97920892494929	0.0
34	3.426347239734664E-4	0.0	0.0	3.066580779562524	0.0
35	3.426347239734664E-4	0.0	0.0	3.1690285620305905	0.0
36	3.426347239734664E-4	0.0	0.0	3.2574283208157446	0.0
37	3.426347239734664E-4	0.0	0.0	3.3547365824242092	0.0
38	3.426347239734664E-4	0.0	0.0	3.47739981360671	0.0
39	3.426347239734664E-4	0.0	0.0	3.7192999287319775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	25	3.8836304E-5	45.000004	3
ATTAACG	35	1.2077726E-7	45.0	1
TAACGAG	20	7.0246286E-4	45.0	1
CGGGGTA	45	3.8198777E-10	45.0	6
TGCATAC	20	7.0246286E-4	45.0	35
GCGATAT	20	7.0246286E-4	45.0	9
CCTATCC	20	7.0246286E-4	45.0	20
CGGGTAC	30	2.159919E-6	44.999996	6
CGAGGGT	30	2.159919E-6	44.999996	4
GCGATCA	30	2.159919E-6	44.999996	9
TAGGGTG	95	0.0	42.63158	5
TACGGGA	90	0.0	42.5	4
ACGGGAG	135	0.0	41.666664	5
TTAACGG	65	0.0	41.53846	2
CGTTTTT	585	0.0	41.153847	1
AGGGCGA	230	0.0	41.086956	6
CGAAAGG	55	6.002665E-11	40.909092	2
TAGACGG	55	6.002665E-11	40.909092	2
TATTGCG	55	6.002665E-11	40.909092	1
CGAGGGC	105	0.0	40.714287	4
>>END_MODULE