FastQCFastQC Report
Thu 26 May 2016
SRR1547482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547482_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3077626
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131840.4283821361010077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT351.2128112E-745.045
CGTACGT207.0351333E-445.015
TATAGCG2050.043.902441
CGGGATA5400.042.5000046
CGGTCTA4300.041.86046631
TAGGGTA12650.040.909095
CGTTTTT68350.039.8975871
ACGGGAT10100.039.876245
CGTTAGG4550.039.560442
CGACATA403.4607365E-739.37523
ACCGTTC9200.039.13043645
CGTCATA5750.039.13043638
TACGACG750.039.01
GTAATCG356.2517065E-638.5714262
TAGGGAC27800.038.525185
TCACGAC4800.038.43749625
CGACGGT4700.038.2978728
GTTGATC22800.038.28947416
CGGTAGT12200.037.80737712
GGGCGAT41750.037.7784427
Produced by FastQC (version 0.11.5)