##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547482_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3077626
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.545462639060105	31.0	31.0	33.0	30.0	34.0
2	31.981592305237868	31.0	31.0	34.0	30.0	34.0
3	32.07513875955038	33.0	31.0	34.0	30.0	34.0
4	35.80301310165693	37.0	35.0	37.0	35.0	37.0
5	35.7530970299835	37.0	35.0	37.0	35.0	37.0
6	35.80482846193787	37.0	35.0	37.0	35.0	37.0
7	36.09245210431677	37.0	35.0	37.0	35.0	37.0
8	36.08976171893531	37.0	35.0	37.0	35.0	37.0
9	37.94203454220883	39.0	38.0	39.0	35.0	39.0
10	37.41151816367551	39.0	37.0	39.0	35.0	39.0
11	37.09330925849989	39.0	37.0	39.0	33.0	39.0
12	36.60902721773211	39.0	35.0	39.0	33.0	39.0
13	36.39506684697881	39.0	35.0	39.0	32.0	39.0
14	37.52955817243551	40.0	35.0	41.0	33.0	41.0
15	37.64568761766375	40.0	35.0	41.0	33.0	41.0
16	37.71923034182841	40.0	35.0	41.0	33.0	41.0
17	37.62383311032595	40.0	35.0	41.0	33.0	41.0
18	37.50446285546067	39.0	36.0	41.0	32.0	41.0
19	37.484414935408005	39.0	36.0	41.0	32.0	41.0
20	37.385397705894086	39.0	35.0	41.0	32.0	41.0
21	37.2510704679516	39.0	35.0	41.0	32.0	41.0
22	37.17862046915382	39.0	35.0	41.0	32.0	41.0
23	37.07676663766163	39.0	35.0	41.0	32.0	41.0
24	36.93381521991301	39.0	35.0	41.0	31.0	41.0
25	36.80335199923577	38.0	35.0	41.0	31.0	41.0
26	36.76416465158535	39.0	35.0	41.0	31.0	41.0
27	36.71017433567302	39.0	35.0	41.0	31.0	41.0
28	36.69708177666812	38.0	35.0	41.0	31.0	41.0
29	36.62626420494238	38.0	35.0	41.0	31.0	41.0
30	36.56719594908543	38.0	35.0	41.0	31.0	41.0
31	36.49049527135526	38.0	35.0	41.0	30.0	41.0
32	36.3704559943281	38.0	35.0	41.0	30.0	41.0
33	36.276442621683074	38.0	35.0	41.0	30.0	41.0
34	36.140544367639215	38.0	35.0	41.0	30.0	41.0
35	35.9648189871024	38.0	35.0	41.0	29.0	41.0
36	35.855583491951265	38.0	35.0	41.0	29.0	41.0
37	35.80598097364657	38.0	35.0	41.0	29.0	41.0
38	35.66179873707851	38.0	35.0	41.0	28.0	41.0
39	35.56939667133044	38.0	35.0	40.0	28.0	41.0
40	35.400683838777034	38.0	34.0	40.0	27.0	41.0
41	35.3139517277278	38.0	34.0	40.0	27.0	41.0
42	35.263766942442	38.0	34.0	40.0	27.0	41.0
43	35.1583990387396	38.0	34.0	40.0	26.0	41.0
44	35.00151870305229	37.0	34.0	40.0	26.0	41.0
45	34.87689309877159	37.0	34.0	40.0	26.0	41.0
46	34.81582459987016	37.0	34.0	40.0	25.0	41.0
47	34.72125105519644	37.0	34.0	40.0	25.0	41.0
48	34.56228534591273	36.0	34.0	40.0	24.0	41.0
49	34.45509200923049	36.0	34.0	40.0	24.0	41.0
50	34.34523948004078	36.0	33.0	40.0	24.0	41.0
51	33.96623176435343	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	10.0
11	14.0
12	31.0
13	41.0
14	83.0
15	150.0
16	352.0
17	708.0
18	1471.0
19	2759.0
20	4602.0
21	7177.0
22	10701.0
23	15521.0
24	21653.0
25	29963.0
26	39508.0
27	46865.0
28	52316.0
29	59612.0
30	70363.0
31	84560.0
32	105124.0
33	136363.0
34	230883.0
35	344075.0
36	226022.0
37	296706.0
38	451517.0
39	838255.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.461904078013376	24.02263952799983	29.701919596468183	13.813536797518609
2	29.854829664163223	25.320133115589744	31.000452946524366	13.824584273722667
3	29.765670032681037	24.658649231583045	31.548115333052163	14.027565402683756
4	25.68073573592113	28.134250230534835	30.947879956823865	15.237134076720174
5	23.725494910687654	33.097913781596596	28.65699081044935	14.5196004972664
6	22.89758404692448	41.25540270325244	25.334917238156944	10.512096011666134
7	89.93789368818693	3.444830528465772	5.2110945254556595	1.4061812578916346
8	90.78695721962318	2.6359603148660686	4.9386767592943395	1.638405706216415
9	86.19949272588677	4.804774849185703	6.5487164457279725	2.447015979199552
10	45.15340720412422	31.11391702565549	12.678863513630311	11.053812256589982
11	35.463275914617306	23.447195988076526	23.638967177948196	17.450560919357972
12	32.53842409701504	20.771107340528054	27.353096185176497	19.33737237728041
13	25.124365338738365	27.591981611800787	27.841622081435496	19.442030968025353
14	20.384283210500563	30.636601068485902	29.666600165192264	19.31251555582127
15	19.006467972391707	25.126054952746046	37.397364072177716	18.47011300268454
16	24.127493074207198	22.436741826329776	34.70558150990406	18.730183589558965
17	23.774201283716735	22.19376233499457	27.905892398881477	26.126143982407218
18	24.066082103543447	24.698842549419588	30.473390853859435	20.761684493177533
19	28.500019170620472	25.14746756103568	26.05647989716749	20.296033371176357
20	29.685153426699674	25.824775330075845	25.121700947418564	19.368370295805924
21	27.43143578849412	25.1753461921624	28.344899607684624	19.048318411658858
22	26.21029975702051	22.987815933450005	27.056146523326746	23.745737786202742
23	23.77871775192957	26.7756381054748	26.93998556029875	22.505658582296874
24	22.855701115080258	25.443214997533815	31.635260424755963	20.06582346262996
25	23.58753142844517	25.50134421791342	29.03036301356955	21.88076134007186
26	22.13998711994245	29.252189837231686	25.518597776337998	23.08922526648787
27	21.453029055512268	28.820785891463096	28.405010875265546	21.32117417775909
28	20.559223245449576	26.86898927939912	31.803117077903554	20.76867039724775
29	21.165209807819405	25.484740511030257	31.46717632356888	21.882873357581463
30	22.805175157735217	25.810316133279354	30.74236440685125	20.642144302134177
31	27.399430600079413	25.40630342998142	26.27206814603204	20.92219782390713
32	28.517435191930403	25.527208309261752	25.613183668191002	20.342172830616846
33	26.108240572441225	25.461475825847586	26.382282967456085	22.048000634255104
34	22.485123273588147	26.41977940139575	28.033263300998883	23.061834024017212
35	22.61242919055142	26.01872352261126	29.15955350000292	22.209293786834397
36	27.648161277556145	25.049177515396607	27.01722041599597	20.285440791051286
37	23.2381387472032	27.85991540232634	28.076770861696644	20.825174988773814
38	23.329962770005192	29.217260316880612	25.66059033813725	21.792186574976945
39	22.70811983002483	27.802371048334006	26.443596460388623	23.045912661252537
40	24.55392565568396	24.831022352943467	27.186214309341032	23.42883768203154
41	21.47548142626817	24.788489569557832	28.047202616562245	25.68882638761175
42	23.75057918018629	26.200519491322204	25.398277763444945	24.650623565046565
43	23.267154618527396	25.362860854437802	26.702042418409516	24.667942108625283
44	21.613184967894085	26.82964076856642	28.198390577672534	23.358783685866964
45	21.6764155228738	29.17160824609618	26.0552451792388	23.09673105179122
46	23.542464224048015	28.077160772621497	26.713349835230144	21.66702516810035
47	23.08916028133373	26.81488913857629	26.633158155019487	23.462792425070493
48	23.314788736513144	25.848527403914577	28.253530480961626	22.583153378610657
49	22.884716986404456	25.022046213542517	29.522917989385324	22.570318810667704
50	22.26193176168904	27.541260698993312	28.049931993036193	22.146875546281453
51	21.1358690107245	28.19835808509546	27.58161647971521	23.08415642446483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3189.0
1	4720.0
2	6251.0
3	15007.5
4	23764.0
5	17475.5
6	11187.0
7	10832.0
8	10477.0
9	10969.5
10	11462.0
11	11163.5
12	10865.0
13	10877.0
14	10889.0
15	10451.5
16	10014.0
17	9934.5
18	9855.0
19	9570.5
20	9286.0
21	10562.0
22	11838.0
23	13178.5
24	14519.0
25	15096.0
26	20861.5
27	26050.0
28	34070.0
29	42090.0
30	46431.5
31	50773.0
32	61023.0
33	71273.0
34	73997.5
35	76722.0
36	84187.0
37	91652.0
38	102325.5
39	112999.0
40	137428.0
41	161857.0
42	184344.0
43	206831.0
44	219008.0
45	231185.0
46	228318.5
47	225452.0
48	221787.5
49	218123.0
50	207224.5
51	196326.0
52	191939.5
53	187553.0
54	177241.5
55	166930.0
56	170456.5
57	173983.0
58	164418.5
59	154854.0
60	146170.0
61	137486.0
62	126049.5
63	114613.0
64	100098.5
65	85584.0
66	73776.5
67	61969.0
68	53117.5
69	44266.0
70	36228.0
71	28190.0
72	24330.5
73	20471.0
74	17130.5
75	10596.5
76	7403.0
77	6116.5
78	4830.0
79	3638.0
80	2446.0
81	1788.5
82	1131.0
83	868.0
84	605.0
85	541.0
86	477.0
87	331.0
88	185.0
89	168.5
90	152.0
91	98.0
92	44.0
93	37.0
94	30.0
95	21.5
96	13.0
97	9.5
98	6.0
99	9.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3077626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.07129894446234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.59311950453922	14.099718338176922
2	6.963773570014216	2.377613207905169
3	2.008190025126049	1.0284723676864238
4	0.9396626644387975	0.6416504900633886
5	0.5740936042966114	0.4900261770525662
6	0.42059016188913145	0.4308012232025505
7	0.3279706000454704	0.3919218910859644
8	0.2508789383687076	0.34262634846092405
9	0.2076751293123888	0.31907557942394965
>10	2.5500542954177736	10.762111237703808
>50	1.3753586791319972	17.326081871639428
>100	1.780391164624214	50.095628279696946
>500	0.005558330722484378	0.6240680693353693
>1k	0.0024916654962861004	0.6388247777331174
>5k	0.0	0.0
>10k+	1.9166657663739235E-4	0.4313801408335085
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13184	0.4283821361010077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018163350582559416	0.0
2	0.0	0.0	0.0	0.06621987207022556	0.0
3	0.0	0.0	0.0	0.09383856258037851	0.0
4	0.0	0.0	0.0	0.16434745482394547	0.0
5	0.0	0.0	0.0	0.2766418011805203	0.0
6	0.0	0.0	0.0	0.44888495223266245	0.0
7	0.0	0.0	0.0	0.5288166918267522	0.0
8	0.0	0.0	0.0	0.7347546453012809	0.0
9	0.0	0.0	0.0	0.8139715481998138	0.0
10	0.0	0.0	0.0	0.952000015596437	0.0
11	0.0	0.0	0.0	1.1186869359694778	0.0
12	0.0	0.0	0.0	1.2486572442525505	0.0
13	0.0	0.0	0.0	1.3048369100079087	0.0
14	0.0	0.0	0.0	1.3282640580759326	0.0
15	0.0	0.0	0.0	1.3583521844434638	0.0
16	0.0	0.0	0.0	1.4198931254154987	0.0
17	0.0	0.0	0.0	1.4969005330732195	0.0
18	0.0	0.0	0.0	1.5966852372575484	0.0
19	0.0	0.0	0.0	1.654619502174728	0.0
20	0.0	0.0	0.0	1.7198645969328308	0.0
21	0.0	0.0	0.0	1.8020708169218742	0.0
22	0.0	0.0	0.0	1.8948046318818466	0.0
23	0.0	0.0	0.0	1.9928022443272835	0.0
24	0.0	0.0	0.0	2.0685099489021734	0.0
25	0.0	0.0	0.0	2.130180860182491	0.0
26	0.0	0.0	0.0	2.189122394988865	0.0
27	0.0	0.0	0.0	2.2550173412883825	0.0
28	0.0	0.0	0.0	2.322926827366288	0.0
29	0.0	0.0	0.0	2.3988294874036025	0.0
30	0.0	0.0	0.0	2.4926355574069103	0.0
31	0.0	0.0	0.0	2.575914032439289	0.0
32	0.0	0.0	0.0	2.656658086460148	0.0
33	0.0	0.0	0.0	2.7402289946861638	0.0
34	0.0	0.0	0.0	2.8265942645402657	0.0
35	0.0	0.0	0.0	2.931837721672484	0.0
36	0.0	0.0	0.0	3.0194702020323456	0.0
37	0.0	0.0	0.0	3.1159406633554565	0.0
38	0.0	0.0	0.0	3.218292281128376	0.0
39	0.0	0.0	0.0	3.3657435958755224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	35	1.2128112E-7	45.0	45
CGTACGT	20	7.0351333E-4	45.0	15
TATAGCG	205	0.0	43.90244	1
CGGGATA	540	0.0	42.500004	6
CGGTCTA	430	0.0	41.860466	31
TAGGGTA	1265	0.0	40.90909	5
CGTTTTT	6835	0.0	39.897587	1
ACGGGAT	1010	0.0	39.87624	5
CGTTAGG	455	0.0	39.56044	2
CGACATA	40	3.4607365E-7	39.375	23
ACCGTTC	920	0.0	39.130436	45
CGTCATA	575	0.0	39.130436	38
TACGACG	75	0.0	39.0	1
GTAATCG	35	6.2517065E-6	38.571426	2
TAGGGAC	2780	0.0	38.52518	5
TCACGAC	480	0.0	38.437496	25
CGACGGT	470	0.0	38.29787	28
GTTGATC	2280	0.0	38.289474	16
CGGTAGT	1220	0.0	37.807377	12
GGGCGAT	4175	0.0	37.778442	7
>>END_MODULE