FastQCFastQC Report
Thu 26 May 2016
SRR1547481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547481_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2596760
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115860.4461713828001047No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43110.16601457200511407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA302.166562E-645.00000418
TTACGAT302.166562E-645.00000419
CCGTTTA453.8562575E-1045.00000444
CGATACG453.8562575E-1045.00000410
TATCCGT453.8562575E-1045.00000441
ACTATCC207.0349296E-445.043
GGCGTAA502.1827873E-1145.035
ATCACGC207.0349296E-445.017
CATTACG207.0349296E-445.019
CCGTACT253.892168E-545.019
TACGATA207.0349296E-445.017
TACGACT207.0349296E-445.020
CGAATCG253.892168E-545.043
GCGATCG207.0349296E-445.09
ACCGTAT406.8212103E-945.013
GTACGAA207.0349296E-445.038
CCCGATA207.0349296E-445.033
CCGTTAT650.044.99999625
TACGCAC650.041.5384630
CGGGTAT2450.041.326536