##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547481_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2596760
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6153310278963	31.0	31.0	33.0	30.0	34.0
2	32.054220644187374	33.0	31.0	34.0	30.0	34.0
3	32.14109351653599	33.0	31.0	34.0	30.0	34.0
4	35.866026895053835	37.0	35.0	37.0	35.0	37.0
5	35.808646929250294	37.0	35.0	37.0	35.0	37.0
6	35.86492090143101	37.0	35.0	37.0	35.0	37.0
7	36.1180097506123	37.0	35.0	37.0	35.0	37.0
8	36.113058965788134	37.0	35.0	37.0	35.0	37.0
9	37.99287650764799	39.0	39.0	39.0	35.0	39.0
10	37.50392835687549	39.0	37.0	39.0	35.0	39.0
11	37.16636385341734	39.0	37.0	39.0	34.0	39.0
12	36.442830681310554	38.0	35.0	39.0	33.0	39.0
13	36.12276336665691	38.0	35.0	39.0	32.0	39.0
14	37.07877277838537	39.0	35.0	41.0	32.0	41.0
15	37.290277114558144	39.0	35.0	41.0	32.0	41.0
16	37.42560999091175	39.0	35.0	41.0	33.0	41.0
17	37.38660484603891	39.0	35.0	41.0	33.0	41.0
18	37.3021480614304	39.0	35.0	41.0	33.0	41.0
19	37.26594910580878	39.0	35.0	41.0	32.0	41.0
20	37.11481808099324	39.0	35.0	41.0	32.0	41.0
21	36.99237318812674	39.0	35.0	41.0	32.0	41.0
22	36.953359571157904	38.0	35.0	41.0	32.0	41.0
23	36.87954142854942	38.0	35.0	41.0	32.0	41.0
24	36.75456954050432	38.0	35.0	41.0	32.0	41.0
25	36.61281712595696	38.0	35.0	41.0	31.0	41.0
26	36.574468953619125	38.0	35.0	41.0	31.0	41.0
27	36.53329495217117	38.0	35.0	41.0	31.0	41.0
28	36.52727398758453	38.0	35.0	41.0	31.0	41.0
29	36.45019601349374	38.0	35.0	40.0	31.0	41.0
30	36.418723717247644	38.0	35.0	40.0	31.0	41.0
31	36.31503103867897	38.0	35.0	41.0	31.0	41.0
32	36.185629014618215	38.0	35.0	41.0	30.0	41.0
33	36.09483587239483	38.0	35.0	41.0	30.0	41.0
34	35.97285270876015	38.0	35.0	41.0	30.0	41.0
35	35.78191400052373	38.0	35.0	41.0	30.0	41.0
36	35.61872140667601	38.0	35.0	40.0	29.0	41.0
37	35.545514795360376	38.0	34.0	40.0	29.0	41.0
38	35.37690814707559	38.0	34.0	40.0	27.0	41.0
39	35.28836203576765	37.0	34.0	40.0	27.0	41.0
40	35.06211124632234	37.0	34.0	40.0	26.0	41.0
41	35.01230148338699	37.0	34.0	40.0	26.0	41.0
42	34.98068708698532	37.0	34.0	40.0	26.0	41.0
43	34.89811418845022	37.0	34.0	40.0	26.0	41.0
44	34.78244196614242	37.0	34.0	40.0	26.0	41.0
45	34.68864200003081	36.0	34.0	40.0	25.0	41.0
46	34.6748344090328	36.0	34.0	40.0	25.0	41.0
47	34.58053535944793	36.0	34.0	40.0	25.0	41.0
48	34.447200357368416	36.0	34.0	40.0	24.0	41.0
49	34.333269150787906	36.0	34.0	40.0	24.0	41.0
50	34.21825390101511	35.0	34.0	40.0	24.0	41.0
51	33.85963007748117	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	11.0
11	12.0
12	13.0
13	33.0
14	58.0
15	127.0
16	262.0
17	609.0
18	1219.0
19	2424.0
20	3931.0
21	6408.0
22	9608.0
23	13587.0
24	19093.0
25	26703.0
26	35061.0
27	41562.0
28	45449.0
29	51010.0
30	59172.0
31	71831.0
32	89611.0
33	118646.0
34	220692.0
35	359469.0
36	168246.0
37	223277.0
38	344336.0
39	684039.0
40	257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.889369830095966	23.893313205687086	29.973929050046983	13.243387914169965
2	29.54008071596913	25.588425576487623	31.381490780819178	13.49000292672407
3	29.431252791940725	24.925946179084704	31.450692401300085	14.192108627674488
4	25.786249018007055	27.81127250881868	31.041798240884795	15.36068023228947
5	23.3739737210986	33.01787612255272	29.497296631186554	14.110853525162126
6	22.624809377840077	42.07797409079006	24.809300821023122	10.48791571034674
7	89.6052773456153	3.739275096658914	5.2487715460805004	1.406676011645281
8	90.54918436821269	2.732559035105285	5.092615413053189	1.6256411836288298
9	86.49798210077174	4.908462853710008	6.334201081347525	2.2593539641707356
10	48.88572682881745	28.510528504752074	12.582679955020874	10.021064711409602
11	41.45007624886397	22.426215745775504	21.758036938338545	14.365671067021982
12	38.76711748486576	20.98665259785271	24.32307952987569	15.923150387405844
13	25.037931884348186	31.14565843589704	26.535836966065407	17.280572713689367
14	18.262950754016543	35.32259430983225	29.285070626472994	17.129384309678215
15	17.097999044963725	24.93153006053698	41.970416981161144	16.00005391333816
16	21.794659498759994	21.22456445724672	39.19615212803647	17.784623915956807
17	21.790577488870746	21.319259384771794	28.141915309847654	28.748247816509803
18	23.68385988693603	24.91585668294336	31.12609559605046	20.274187834070148
19	28.78306042915017	25.82279455937399	25.668448374127763	19.72569663734808
20	29.38119040650657	26.951585822332447	25.370654199849042	18.296569571311945
21	25.39568539256612	25.00581493861581	29.80968591629569	19.788813752522376
22	24.806220058842555	23.56582818589319	27.581948274002986	24.046003481261263
23	21.652559343181505	28.046796777522758	27.366256411836286	22.934387467459448
24	21.89798056039064	25.679423589396016	33.13644695697715	19.286148893236188
25	22.439347494570157	26.199802831220442	30.371501409448697	20.9893482647607
26	19.73170412360018	30.523537023059504	26.265423065666443	23.47933578767387
27	19.490095349589488	31.45723902093378	28.811441950738615	20.24122367873812
28	17.67402455367458	29.30563471402825	33.50471356613626	19.51562716616091
29	18.537408154777495	26.208120889107967	33.44475423219704	21.809716723917496
30	20.787327284770253	28.13197985181534	31.262188265376857	19.818504598037553
31	27.190961043762226	26.5153113880374	26.869830095965742	19.42389747223463
32	29.30189928988432	25.846054313837243	26.24801675934626	18.604029636932175
33	26.732582140821638	26.652482324126993	26.318989818080997	20.29594571697038
34	21.754494061830897	27.34368982886366	28.55920454720498	22.342611562100466
35	21.600301914693695	27.630239221183324	28.93055191854465	21.838906945578337
36	29.666083889154176	25.47281997566198	25.646767510282043	19.2143286249018
37	21.958748594402255	30.122652844313684	28.57430028188974	19.344298279394323
38	22.865301375560314	30.39295121613087	24.874458941142038	21.867288467166777
39	21.528019532032225	28.586700349666504	27.32470463192594	22.560575486375328
40	24.43833854495602	25.53285632865571	26.694342180255394	23.334462946132874
41	19.744027172322433	24.972350159429443	28.668417566505955	26.615205101742173
42	23.40458879526795	25.585883947688657	26.219981823503137	24.78954543354026
43	21.980891572575057	26.32253269458864	27.36702660238143	24.329549130454875
44	20.925499468568525	29.34187217917713	27.626657835148414	22.105970517105934
45	19.296507956068332	31.888853802430724	25.177875506400284	23.636762735100664
46	22.34018546188327	30.307999199001834	25.96366241008025	21.388152929034643
47	21.73916726998259	27.99361512038078	27.89141083504059	22.375806774596036
48	23.21974306443414	25.754324619910967	28.896201420231364	22.129730895423528
49	22.215645650733993	25.829379688534942	29.728623361419615	22.22635129931145
50	20.877170011860933	29.836064942466766	27.57074200157119	21.71602304410111
51	19.130685931699503	30.966858700842586	26.366125479443614	23.536329888014297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2832.0
1	4249.5
2	5667.0
3	12992.5
4	20318.0
5	14868.5
6	9419.0
7	9530.5
8	9642.0
9	9807.5
10	9973.0
11	9937.5
12	9902.0
13	9687.5
14	9473.0
15	9210.5
16	8948.0
17	8601.5
18	8255.0
19	8654.0
20	9053.0
21	9669.0
22	10285.0
23	11302.0
24	12319.0
25	15232.5
26	19880.5
27	21615.0
28	25017.0
29	28419.0
30	38529.5
31	48640.0
32	57325.0
33	66010.0
34	71196.5
35	76383.0
36	80154.0
37	83925.0
38	92064.5
39	100204.0
40	126617.0
41	153030.0
42	182039.5
43	211049.0
44	216398.5
45	221748.0
46	216107.5
47	210467.0
48	197570.0
49	184673.0
50	174803.5
51	164934.0
52	152026.5
53	139119.0
54	136877.0
55	134635.0
56	133995.0
57	133355.0
58	127400.0
59	121445.0
60	111951.0
61	102457.0
62	90845.5
63	79234.0
64	68143.5
65	57053.0
66	49061.0
67	41069.0
68	33048.0
69	25027.0
70	21006.0
71	16985.0
72	14441.5
73	11898.0
74	10397.5
75	7313.0
76	5729.0
77	3858.5
78	1988.0
79	1555.5
80	1123.0
81	964.0
82	805.0
83	553.5
84	302.0
85	235.0
86	168.0
87	120.0
88	72.0
89	51.5
90	31.0
91	23.0
92	15.0
93	16.0
94	17.0
95	10.5
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2596760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.534260296244057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.92767429010408	13.711477524747268
2	7.63272926264234	2.524030647985748
3	2.204570887725259	1.0935284669751377
4	0.9845119871782878	0.6511270984311319
5	0.5857236679502874	0.48422537937804366
6	0.40446148192866493	0.40124828532078943
7	0.2953305746997157	0.3418150816867113
8	0.23969419696229863	0.3170532995259069
9	0.19191370796732474	0.2855836081754202
>10	1.9137955888232792	7.127266600052675
>50	0.7870994471615597	9.56788945971567
>100	1.8208170142604463	61.44136944751043
>500	0.00910875622528089	0.9836538333328972
>1k	0.0023355785193027927	0.6223139731532521
>5k	0.0	0.0
>10k+	2.3355785193027924E-4	0.4474172940089097
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11586	0.4461713828001047	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4311	0.16601457200511407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.701905451408678E-5	0.0	0.0	0.0306150741693495	0.0
2	7.701905451408678E-5	0.0	0.0	0.08368120272955529	0.0
3	7.701905451408678E-5	0.0	0.0	0.11957208213311973	0.0
4	7.701905451408678E-5	0.0	0.0	0.21630801460281274	0.0
5	7.701905451408678E-5	0.0	0.0	0.35401808407399993	0.0
6	7.701905451408678E-5	0.0	0.0	0.5374004528720405	0.0
7	7.701905451408678E-5	0.0	0.0	0.6180008934210324	0.0
8	7.701905451408678E-5	0.0	0.0	0.8184429827939432	0.0
9	7.701905451408678E-5	0.0	0.0	0.8832160076402902	0.0
10	7.701905451408678E-5	0.0	0.0	1.0130316240237836	0.0
11	7.701905451408678E-5	0.0	0.0	1.1816263343551194	0.0
12	7.701905451408678E-5	0.0	0.0	1.330311619094564	0.0
13	7.701905451408678E-5	0.0	0.0	1.3917728245968053	0.0
14	7.701905451408678E-5	0.0	0.0	1.4167655077866264	0.0
15	7.701905451408678E-5	0.0	0.0	1.450268796500254	0.0
16	7.701905451408678E-5	0.0	0.0	1.5256704508695451	0.0
17	7.701905451408678E-5	0.0	0.0	1.6113541490164667	0.0
18	7.701905451408678E-5	0.0	0.0	1.7363945840200865	0.0
19	7.701905451408678E-5	0.0	0.0	1.8000508325759792	0.0
20	7.701905451408678E-5	0.0	0.0	1.8724102342919637	0.0
21	7.701905451408678E-5	0.0	0.0	1.9547821130947796	0.0
22	7.701905451408678E-5	0.0	0.0	2.050709345492075	0.0
23	7.701905451408678E-5	0.0	0.0	2.149524792433648	0.0
24	7.701905451408678E-5	0.0	0.0	2.22400221814877	0.0
25	7.701905451408678E-5	0.0	0.0	2.286772747577751	0.0
26	7.701905451408678E-5	0.0	0.0	2.3514302438423265	0.0
27	7.701905451408678E-5	0.0	0.0	2.4337636131178852	0.0
28	7.701905451408678E-5	0.0	0.0	2.5082410388330074	0.0
29	7.701905451408678E-5	0.0	0.0	2.5986228993052882	0.0
30	7.701905451408678E-5	0.0	0.0	2.7186185862382355	0.0
31	1.1552858177113018E-4	0.0	0.0	2.8138526471449037	0.0
32	1.1552858177113018E-4	0.0	0.0	2.902809655108674	0.0
33	1.1552858177113018E-4	0.0	0.0	2.991381567799874	0.0
34	1.1552858177113018E-4	0.0	0.0	3.1002480013555354	0.0
35	1.1552858177113018E-4	0.0	0.0	3.2293704462484016	0.0
36	1.1552858177113018E-4	0.0	0.0	3.328763536098831	0.0
37	1.1552858177113018E-4	0.0	0.0	3.4437144749611055	0.0
38	1.1552858177113018E-4	0.0	0.0	3.591437021519124	0.0
39	1.1552858177113018E-4	0.0	0.0	3.9828093470324557	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGA	30	2.166562E-6	45.000004	18
TTACGAT	30	2.166562E-6	45.000004	19
CCGTTTA	45	3.8562575E-10	45.000004	44
CGATACG	45	3.8562575E-10	45.000004	10
TATCCGT	45	3.8562575E-10	45.000004	41
ACTATCC	20	7.0349296E-4	45.0	43
GGCGTAA	50	2.1827873E-11	45.0	35
ATCACGC	20	7.0349296E-4	45.0	17
CATTACG	20	7.0349296E-4	45.0	19
CCGTACT	25	3.892168E-5	45.0	19
TACGATA	20	7.0349296E-4	45.0	17
TACGACT	20	7.0349296E-4	45.0	20
CGAATCG	25	3.892168E-5	45.0	43
GCGATCG	20	7.0349296E-4	45.0	9
ACCGTAT	40	6.8212103E-9	45.0	13
GTACGAA	20	7.0349296E-4	45.0	38
CCCGATA	20	7.0349296E-4	45.0	33
CCGTTAT	65	0.0	44.999996	25
TACGCAC	65	0.0	41.53846	30
CGGGTAT	245	0.0	41.32653	6
>>END_MODULE