FastQCFastQC Report
Thu 26 May 2016
SRR1547480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547480_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3060975
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442921.446989929679269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG406.8212103E-945.01
TACGTAC406.8212103E-945.016
CGTTTTT197500.043.177221
CGACGGT4150.040.6626528
TACGGGA16800.040.446434
TACGTAG1950.040.3846171
CGGTCTA4200.040.1785731
AGGGCGA26950.039.2393346
ACGGGAT18400.038.885875
GGGCGAT49600.038.7852867
ATAGGGA59450.038.490334
TATGGGA55250.038.1990974
CGCGTCA659.094947E-1238.07692739
CGTATGG3900.038.0769232
AGGGATT73150.038.048536
GGCGATA11600.037.629318
AAGGGAT63500.037.2755935
GCGCGAC4650.037.258069
TAGGGAT68850.037.2548985
TAGGGAC47250.037.2380945
Produced by FastQC (version 0.11.5)