##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547480_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3060975
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27974093221931	31.0	31.0	33.0	30.0	34.0
2	31.687880495593724	31.0	31.0	34.0	30.0	34.0
3	31.74861702562092	31.0	31.0	34.0	30.0	34.0
4	35.54374864218101	37.0	35.0	37.0	33.0	37.0
5	35.43999019920123	37.0	35.0	37.0	33.0	37.0
6	35.49250908615719	37.0	35.0	37.0	33.0	37.0
7	35.95062030888851	37.0	35.0	37.0	35.0	37.0
8	35.99395094700218	37.0	35.0	37.0	35.0	37.0
9	37.72974852783835	39.0	37.0	39.0	35.0	39.0
10	37.03479838940207	39.0	37.0	39.0	33.0	39.0
11	36.78412956655968	39.0	37.0	39.0	32.0	39.0
12	36.57426244905626	39.0	35.0	39.0	32.0	39.0
13	36.53269268778739	39.0	35.0	39.0	32.0	39.0
14	37.64324308431137	40.0	36.0	41.0	32.0	41.0
15	37.72035086859579	40.0	36.0	41.0	32.0	41.0
16	37.73512687950735	40.0	36.0	41.0	32.0	41.0
17	37.65783451351285	40.0	36.0	41.0	32.0	41.0
18	37.50816128847834	39.0	36.0	41.0	32.0	41.0
19	37.40395756254135	39.0	36.0	41.0	32.0	41.0
20	37.24691152328915	39.0	35.0	41.0	32.0	41.0
21	37.1938960625291	39.0	35.0	41.0	32.0	41.0
22	37.12686611292153	39.0	35.0	41.0	32.0	41.0
23	37.0382270355034	39.0	35.0	41.0	31.0	41.0
24	36.95506464443519	39.0	35.0	41.0	31.0	41.0
25	36.85397985935854	39.0	35.0	41.0	31.0	41.0
26	36.72935616919445	38.0	35.0	40.0	31.0	41.0
27	36.6404962471108	38.0	35.0	40.0	31.0	41.0
28	36.51274936907358	38.0	35.0	40.0	30.0	41.0
29	36.535593724221854	38.0	35.0	40.0	30.0	41.0
30	36.453689102328504	38.0	35.0	40.0	30.0	41.0
31	36.339739135406205	38.0	35.0	40.0	30.0	41.0
32	36.19978895613326	38.0	35.0	41.0	30.0	41.0
33	36.01958003577292	38.0	35.0	41.0	29.0	41.0
34	35.81316410620799	38.0	35.0	41.0	27.0	41.0
35	35.66113542253694	38.0	35.0	41.0	27.0	41.0
36	35.60843424072395	38.0	35.0	41.0	26.0	41.0
37	35.55591404699483	38.0	35.0	41.0	26.0	41.0
38	35.42699760697163	38.0	35.0	41.0	25.0	41.0
39	35.34109621934188	38.0	34.0	40.0	25.0	41.0
40	35.2377092266353	38.0	34.0	40.0	24.0	41.0
41	35.1307563766447	38.0	34.0	40.0	24.0	41.0
42	35.083305482730175	38.0	34.0	40.0	24.0	41.0
43	35.06310668986189	38.0	34.0	40.0	24.0	41.0
44	34.934702178227525	38.0	34.0	40.0	24.0	41.0
45	34.81990378882546	38.0	34.0	40.0	23.0	41.0
46	34.80578573820433	38.0	34.0	40.0	23.0	41.0
47	34.762812175859	38.0	34.0	40.0	23.0	41.0
48	34.69308145280507	38.0	34.0	40.0	23.0	41.0
49	34.614112170141865	38.0	34.0	40.0	23.0	41.0
50	34.53829809129444	38.0	33.0	40.0	23.0	41.0
51	34.01477797107131	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	10.0
14	23.0
15	59.0
16	210.0
17	506.0
18	1140.0
19	2405.0
20	4080.0
21	6710.0
22	10576.0
23	16067.0
24	24695.0
25	37298.0
26	50719.0
27	57820.0
28	61748.0
29	67041.0
30	76452.0
31	91026.0
32	110919.0
33	138119.0
34	194437.0
35	254654.0
36	252438.0
37	314773.0
38	460874.0
39	825780.0
40	391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.511789544181184	24.376808043188852	28.14201357410629	15.969388838523674
2	32.29284786710117	25.645619451318613	28.830552356683736	13.23098032489648
3	27.019756776843977	25.029410563627604	34.983657168059196	12.967175491469222
4	23.673502723805324	27.613587174021347	34.37110724524049	14.341802856932839
5	22.25843072877106	31.860207940280468	32.13551237759211	13.745848953356365
6	21.147967559356086	40.275500453286945	28.719770661308896	9.856761326048073
7	87.43312179942664	2.4009996814740404	8.886580256290888	1.2792982628084189
8	87.76873381847288	1.9174609397332552	8.624964267921168	1.6888409738727037
9	81.92951592221432	5.805405140518952	10.113117552413856	2.151961384852865
10	29.853331046480285	45.088476710851936	15.022402992510555	10.03578925015722
11	24.638293354241707	24.87328383929957	33.012847213714586	17.475575592744143
12	25.436176381708442	23.591404699483007	32.04070598420438	18.93171293460417
13	23.006329682535792	25.037447218614982	33.695015477094714	18.261207621754505
14	19.031746420666618	28.312122771339197	33.149110985878686	19.507019822115502
15	17.574694337588515	27.401497888744597	36.954630469049896	18.069177304616993
16	21.981558163657006	26.649809292790692	34.43272160014374	16.935910943408555
17	20.711864683638385	26.53193835297577	32.13743986801591	20.61875709536994
18	21.11823846976862	26.17773748560508	33.74506488945516	18.958959155171147
19	22.281103243247657	27.897058943637237	31.27931459747303	18.542523215642078
20	23.5660206306814	27.9602087570137	31.06882610932791	17.404944502976992
21	23.01443167618161	26.686725634805903	32.86897148784293	17.42987120116956
22	22.104002809562314	24.59719533808672	33.06475877784039	20.23404307451057
23	19.320575960274095	27.899051772719474	32.91310775161509	19.86726451539134
24	18.923970303579743	27.98399202868367	34.09991914341019	18.992118524326397
25	20.179485294718187	28.746134809987012	31.864650969053976	19.20972892624082
26	19.840050964153576	30.13634544548714	30.512663448737737	19.510940141621543
27	18.95552887560336	28.79425673192365	32.711962691626034	19.538251700846953
28	17.822883231650046	28.93172926926878	33.94029680085593	19.30509069822524
29	18.92942608155898	27.03540538553892	33.61379952466126	20.421369008240838
30	19.691209500240937	27.269121766757326	33.49782340594092	19.541845327060823
31	20.91853086026511	27.35304927351579	31.580460474195316	20.147959392023786
32	20.928266320371776	27.722637394947686	31.651189571950113	19.697906712730422
33	20.48641364271188	27.723552136165765	31.836130644647536	19.953903576474815
34	18.887511332173574	28.1843203554423	32.047272519376996	20.880895793007127
35	18.875423680363284	28.382165813180443	31.432370404854666	21.31004010160161
36	20.56991644819053	29.102622530402893	30.32386739519271	20.00359362621387
37	19.581342546084173	29.469335750863696	30.675650732201344	20.27367097085079
38	19.548640547537957	29.840818693390176	30.403025179885496	20.20751557918637
39	19.84305654244154	28.617875023481083	30.48685467865631	21.052213755421068
40	20.917093409779564	28.694353923178074	30.820441199291075	19.56811146775129
41	18.7505288347667	28.885959539035767	30.610736775047165	21.75277485115037
42	20.02950040428295	29.025816937413733	29.59060430091719	21.35407835738613
43	19.86082865753559	28.632412874982645	30.6010013149405	20.905757152541266
44	20.19487254877939	28.8779555533776	30.330401261036105	20.596770636806898
45	19.40156322740303	28.994683066669936	30.095476114636675	21.508277591290355
46	20.07030439647498	28.641919649784793	30.164277721967675	21.123498231772555
47	19.300255637501124	29.082922924885047	30.62432721600144	20.992494221612397
48	19.282940893016114	28.29663750929034	31.130048432280567	21.290373165412984
49	19.81695374839716	27.78042943833256	31.025441240127737	21.377175573142544
50	18.56411764225451	28.11130440464231	31.771086010176496	21.55349194292668
51	18.3922769705731	28.4124502813646	31.25745881622686	21.937813931835446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1744.0
1	2718.0
2	3692.0
3	33204.0
4	62716.0
5	41401.0
6	20086.0
7	20142.0
8	20198.0
9	21745.5
10	23293.0
11	24077.0
12	24861.0
13	25072.0
14	25283.0
15	25081.5
16	24880.0
17	24327.0
18	23774.0
19	23202.0
20	22630.0
21	22435.5
22	22241.0
23	24715.0
24	27189.0
25	29808.0
26	39332.5
27	46238.0
28	52342.0
29	58446.0
30	66802.5
31	75159.0
32	82829.0
33	90499.0
34	101002.0
35	111505.0
36	123859.5
37	136214.0
38	144027.0
39	151840.0
40	167456.0
41	183072.0
42	195524.0
43	207976.0
44	213492.0
45	219008.0
46	232935.5
47	246863.0
48	248037.5
49	249212.0
50	236025.0
51	222838.0
52	200481.5
53	178125.0
54	162370.0
55	146615.0
56	133404.0
57	120193.0
58	107478.0
59	94763.0
60	81278.5
61	67794.0
62	57816.0
63	47838.0
64	38774.5
65	29711.0
66	22970.0
67	16229.0
68	13041.5
69	9854.0
70	8046.0
71	6238.0
72	5094.5
73	3951.0
74	3133.5
75	2038.5
76	1761.0
77	1377.0
78	993.0
79	670.5
80	348.0
81	275.0
82	202.0
83	156.0
84	110.0
85	67.0
86	24.0
87	19.5
88	15.0
89	11.5
90	8.0
91	4.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3060975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.041609792534622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4509502679325	16.630604042817037
2	8.073287122216032	3.5589648898196105
3	3.204579991270276	2.119023051496303
4	1.8266481627622233	1.610490641274208
5	1.183430911028192	1.3042361178653585
6	0.8858674963877393	1.1715567409960874
7	0.6780781001631947	1.0462153024862242
8	0.5490011059857994	0.9680694523047149
9	0.4493607695787061	0.8914171265214212
>10	5.721279041464559	29.9448590633128
>50	1.4380047607461577	22.200588064583545
>100	0.5334888555259267	15.883259444256856
>500	0.004065805083564635	0.6180910035732128
>1k	0.0018070244815842824	0.5825097794388295
>5k	0.0	0.0
>10k+	1.5058537346535688E-4	1.4701152792538257
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44292	1.446989929679269	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06308447471802285	0.0
2	0.0	0.0	0.0	0.17830919886637428	0.0
3	0.0	0.0	0.0	0.2916391019201398	0.0
4	0.0	0.0	0.0	0.46926224487295715	0.0
5	0.0	0.0	0.0	0.791545177598641	0.0
6	0.0	0.0	0.0	1.3594687967069317	0.0
7	0.0	0.0	0.0	1.6626401718406716	0.0
8	0.0	0.0	0.0	2.301162211386895	0.0
9	0.0	0.0	0.0	2.5887176471549096	0.0
10	0.0	0.0	0.0	3.0183193263584314	0.0
11	0.0	0.0	0.0	3.454062839454749	0.0
12	0.0	0.0	0.0	3.822311518388749	0.0
13	0.0	0.0	0.0	4.010388846691005	0.0
14	0.0	0.0	0.0	4.1226406618806095	0.0
15	0.0	0.0	0.0	4.230841480247307	0.0
16	0.0	0.0	0.0	4.415031158372741	0.0
17	0.0	0.0	0.0	4.650675030014947	0.0
18	0.0	0.0	0.0	4.915884644598535	0.0
19	0.0	0.0	0.0	5.089293444082359	0.0
20	0.0	0.0	0.0	5.276750055129493	0.0
21	0.0	0.0	0.0	5.5032465145909395	0.0
22	0.0	0.0	0.0	5.766953340030546	0.0
23	0.0	0.0	0.0	6.041179689478025	0.0
24	0.0	0.0	0.0	6.2494139939071705	0.0
25	0.0	0.0	0.0	6.435302477151888	0.0
26	0.0	0.0	0.0	6.6166825929646595	0.0
27	0.0	0.0	0.0	6.8071447822997575	0.0
28	0.0	0.0	0.0	7.00734243174152	0.0
29	0.0	0.0	0.0	7.232532118034286	0.0
30	0.0	0.0	0.0	7.494540138354609	0.0
31	0.0	0.0	0.0	7.726557714453728	0.0
32	0.0	0.0	0.0	7.9564191148245245	0.0
33	0.0	0.0	0.0	8.17618569246727	0.0
34	0.0	0.0	0.0	8.417775382026969	0.0
35	3.266932921699785E-5	0.0	0.0	8.673641568454496	0.0
36	3.266932921699785E-5	0.0	0.0	8.918661537581979	0.0
37	3.266932921699785E-5	0.0	0.0	9.168712583408881	0.0
38	3.266932921699785E-5	0.0	0.0	9.449244113395242	0.0
39	3.266932921699785E-5	0.0	0.0	9.806287212407812	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGCG	40	6.8212103E-9	45.0	1
TACGTAC	40	6.8212103E-9	45.0	16
CGTTTTT	19750	0.0	43.17722	1
CGACGGT	415	0.0	40.66265	28
TACGGGA	1680	0.0	40.44643	4
TACGTAG	195	0.0	40.384617	1
CGGTCTA	420	0.0	40.17857	31
AGGGCGA	2695	0.0	39.239334	6
ACGGGAT	1840	0.0	38.88587	5
GGGCGAT	4960	0.0	38.785286	7
ATAGGGA	5945	0.0	38.49033	4
TATGGGA	5525	0.0	38.199097	4
CGCGTCA	65	9.094947E-12	38.076927	39
CGTATGG	390	0.0	38.076923	2
AGGGATT	7315	0.0	38.04853	6
GGCGATA	1160	0.0	37.62931	8
AAGGGAT	6350	0.0	37.275593	5
GCGCGAC	465	0.0	37.25806	9
TAGGGAT	6885	0.0	37.254898	5
TAGGGAC	4725	0.0	37.238094	5
>>END_MODULE