FastQCFastQC Report
Thu 26 May 2016
SRR1547479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547479_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1560808
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213131.3655106842097169No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG20720.13275175421960933No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA253.8914503E-545.031
CGTTTTT106100.042.8369451
TATTGCG1250.039.61
AATGCGG1950.039.230772
GCGATCG1150.039.1304369
AACGGGA8100.038.888894
TACGGGA7600.038.7828944
GGGCGAT24300.038.7037057
ATAGGGA32250.038.3720934
TACGCGG1000.038.252
TTAGGGA30950.038.0936974
TAGGGAA21150.038.085115
GACCGAT9650.038.005189
AAGGGAT29700.037.55
AGGGATC21900.037.191786
TCACGAC2000.037.12525
AGGGCGA13800.037.010876
TAGGGAT31850.036.9466255
TAAGGGA27800.036.9064754
GGCGATA6650.036.8796968
Produced by FastQC (version 0.11.5)