##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547479_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1560808
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27695462862825	31.0	31.0	33.0	30.0	34.0
2	31.691551427209497	31.0	31.0	34.0	30.0	34.0
3	31.781024956304684	31.0	31.0	34.0	30.0	34.0
4	35.6026731026494	37.0	35.0	37.0	33.0	37.0
5	35.49654922322284	37.0	35.0	37.0	33.0	37.0
6	35.54792133305314	37.0	35.0	37.0	33.0	37.0
7	35.88868842291941	37.0	35.0	37.0	35.0	37.0
8	35.914043239142806	37.0	35.0	37.0	35.0	37.0
9	37.6812599627885	39.0	37.0	39.0	35.0	39.0
10	37.05519128553929	39.0	37.0	39.0	33.0	39.0
11	36.891878437322205	39.0	37.0	39.0	32.0	39.0
12	36.546219650334955	39.0	35.0	39.0	32.0	39.0
13	36.45310954326221	39.0	35.0	39.0	32.0	39.0
14	37.48900185032368	40.0	36.0	41.0	32.0	41.0
15	37.583908462796195	40.0	36.0	41.0	32.0	41.0
16	37.60074973987832	40.0	36.0	41.0	32.0	41.0
17	37.53873634681524	40.0	36.0	41.0	32.0	41.0
18	37.39016330003434	39.0	36.0	41.0	32.0	41.0
19	37.27317389454693	39.0	36.0	41.0	32.0	41.0
20	37.09909803127611	39.0	35.0	41.0	32.0	41.0
21	37.05320385338876	39.0	35.0	41.0	31.0	41.0
22	36.995226831231	39.0	35.0	41.0	31.0	41.0
23	36.91647915694948	39.0	35.0	41.0	31.0	41.0
24	36.82655393872917	38.0	35.0	41.0	31.0	41.0
25	36.67354216534	38.0	35.0	40.0	31.0	41.0
26	36.580847868539884	38.0	35.0	40.0	31.0	41.0
27	36.501431950630696	38.0	35.0	40.0	30.0	41.0
28	36.39817133177175	38.0	35.0	40.0	30.0	41.0
29	36.408124509869246	38.0	35.0	40.0	30.0	41.0
30	36.33620278727428	38.0	35.0	40.0	30.0	41.0
31	36.18477480894511	38.0	35.0	40.0	30.0	41.0
32	36.03887089251209	38.0	35.0	40.0	29.0	41.0
33	35.837122182869386	38.0	35.0	41.0	28.0	41.0
34	35.5817192121004	38.0	35.0	41.0	26.0	41.0
35	35.39599297287046	38.0	34.0	41.0	25.0	41.0
36	35.32997652497937	38.0	34.0	41.0	25.0	41.0
37	35.28022601114294	38.0	34.0	41.0	25.0	41.0
38	35.167758622457086	38.0	34.0	40.0	24.0	41.0
39	35.05729276118523	38.0	34.0	40.0	24.0	41.0
40	34.94879959610663	38.0	34.0	40.0	23.0	41.0
41	34.82668335887566	38.0	34.0	40.0	23.0	41.0
42	34.79639007488429	38.0	34.0	40.0	23.0	41.0
43	34.77782405010738	38.0	34.0	40.0	23.0	41.0
44	34.6523557029436	38.0	34.0	40.0	23.0	41.0
45	34.53689819631883	38.0	33.0	40.0	23.0	41.0
46	34.52117749268328	38.0	33.0	40.0	23.0	41.0
47	34.48261349249876	38.0	33.0	40.0	23.0	41.0
48	34.41299826756398	37.0	33.0	40.0	23.0	41.0
49	34.32909813378711	37.0	33.0	40.0	23.0	41.0
50	34.254987801190154	37.0	33.0	40.0	23.0	41.0
51	33.737541068472225	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	1.0
12	5.0
13	3.0
14	16.0
15	51.0
16	138.0
17	312.0
18	698.0
19	1328.0
20	2386.0
21	3778.0
22	5878.0
23	8682.0
24	13372.0
25	20192.0
26	28312.0
27	33032.0
28	34567.0
29	36549.0
30	40337.0
31	47682.0
32	56951.0
33	71560.0
34	103690.0
35	128621.0
36	125705.0
37	159622.0
38	235244.0
39	401918.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.707172182613107	24.24308435118221	28.420536030056226	16.62920743614846
2	32.56665778237938	25.723087016468394	28.839293494138936	12.87096170701329
3	27.50780365041696	24.86032875279983	34.75084699719632	12.881020599586881
4	24.31798145575881	27.657277512672923	33.978426558551725	14.04631447301654
5	22.341248891599736	31.52847755777777	32.30358891035925	13.826684640263249
6	21.075173884295825	40.1696429029067	28.932578510617578	9.822604702179897
7	87.43701980000102	2.4150952583533654	8.991048226303299	1.1568367153423098
8	87.84315559633215	2.1008990215324372	8.772699781139	1.283245600996407
9	82.03007672948883	5.775534210485851	10.002575589053874	2.1918134709714456
10	27.906443329352488	44.25931953193474	16.35678443472868	11.47745270398409
11	30.938590781185134	22.948754747541017	28.960576829437063	17.15207764183679
12	30.150665552713722	20.71017062957135	29.899962070927366	19.23920174678756
13	26.30278676172854	22.58234196646865	31.474723348419538	19.64014792338327
14	21.42896499761662	24.71950425676957	32.88597956955628	20.965551176057527
15	20.57242146375467	24.896592021568313	34.988928811231105	19.54205770344591
16	25.382173848416972	24.622759493800647	32.01258578889908	17.982480868883297
17	24.75493462360521	24.32996242971589	30.51374672605471	20.40135622062419
18	23.750583031352992	24.14762097580228	32.88726095714527	19.21453503569946
19	24.333614384344518	25.70642897781149	30.13637808109646	19.82357855674753
20	24.859623989625888	26.919134192033873	30.211018908155264	18.010222910184982
21	25.225908631939355	25.025884029297647	32.194927242812696	17.553280095950303
22	24.1353837243274	22.847973613666767	32.88078994982086	20.13585271218497
23	22.181587998011288	24.46265011455605	32.885018528864535	20.47074335856813
24	21.36982896038462	25.295167631124393	33.3437552857238	19.991248122767182
25	22.278653107877457	26.87652805469987	30.566347686582844	20.27847115083982
26	21.16045022834327	27.21109835418578	29.982803778555724	21.64564763891523
27	19.8704132731252	26.511588869354846	32.53455902327512	21.083438834244827
28	19.497273207210625	26.628195139953153	32.93960564015561	20.93492601268061
29	21.407565824880447	25.49371863803876	31.89886264037601	21.199852896704783
30	22.24110845151998	25.679327630304304	32.008036862958164	20.07152705521755
31	22.950420551406708	25.853147856751118	31.186154863378455	20.01027672846372
32	23.994815505814937	25.69681857089405	30.185134878857617	20.123231044433396
33	23.741485179471145	26.018574994490034	29.925461683948313	20.314478142090504
34	20.8694471068831	26.667661877694115	31.58767766438921	20.875213351033565
35	21.412563236477517	27.977304063023766	30.095373678248706	20.514759022250015
36	22.88116155222167	28.289962634737904	28.223714896386998	20.605160916653425
37	21.20042952111983	29.875551637357063	28.642216082951904	20.281802758571203
38	21.76289460330803	28.774583420894817	28.639076683358876	20.823445292438276
39	21.651221674927346	28.370818191603327	28.333273535245844	21.644686598223483
40	22.834134627705648	27.512544784496235	28.75984746362141	20.893473124176708
41	20.389183038528763	26.984036473416335	29.262535814783114	23.36424467327179
42	20.606442304242417	27.14382550576368	29.313022485789414	22.93670970420449
43	20.52257548654287	27.062713671380468	29.830959349260127	22.583751492816543
44	21.32677433739448	27.059189855510734	29.01567649576373	22.598359311331055
45	21.020714911763648	27.368196472596246	28.738256082746883	22.87283253289322
46	20.94709919477604	28.000369039625628	29.00991025161327	22.042621513985065
47	20.28859411279286	27.445336005453587	30.39592313724686	21.87014674450669
48	20.340490310147054	27.542337045940307	30.460505071732076	21.656667572180563
49	21.299929267405087	26.77651575337902	30.046232464210842	21.877322515005048
50	20.379252284714074	27.50274216944044	30.200960015581675	21.917045530263813
51	19.873104187062086	27.379344544620476	30.25855838770688	22.488992880610557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	767.0
1	1319.5
2	1872.0
3	17550.5
4	33229.0
5	22443.5
6	11658.0
7	11502.5
8	11347.0
9	11653.0
10	11959.0
11	12009.5
12	12060.0
13	11639.5
14	11219.0
15	10734.0
16	10249.0
17	9833.5
18	9418.0
19	9022.0
20	8626.0
21	8699.0
22	8772.0
23	9391.0
24	10010.0
25	11404.5
26	14761.5
27	16724.0
28	19143.0
29	21562.0
30	25374.0
31	29186.0
32	33394.0
33	37602.0
34	42726.0
35	47850.0
36	53509.5
37	59169.0
38	62103.5
39	65038.0
40	71236.5
41	77435.0
42	82707.0
43	87979.0
44	93082.5
45	98186.0
46	101915.5
47	105645.0
48	109421.5
49	113198.0
50	111397.5
51	109597.0
52	105246.5
53	100896.0
54	96515.0
55	92134.0
56	89506.0
57	86878.0
58	81294.0
59	75710.0
60	66244.5
61	56779.0
62	50451.0
63	44123.0
64	37311.0
65	30499.0
66	24941.5
67	19384.0
68	15872.5
69	12361.0
70	9989.0
71	7617.0
72	6242.0
73	4867.0
74	3895.0
75	2302.0
76	1681.0
77	1314.5
78	948.0
79	720.5
80	493.0
81	350.5
82	208.0
83	135.5
84	63.0
85	53.0
86	43.0
87	29.0
88	15.0
89	16.5
90	18.0
91	14.0
92	10.0
93	5.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1560808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.396776142314742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.4011619171499	18.5836371128586
2	6.792523012271168	3.586014187928869
3	3.344988212660538	2.648907151448452
4	2.225209113998404	2.3495338740821756
5	1.7827737031565347	2.352973917731426
6	1.454102204603034	2.3030166229771587
7	1.209896710126851	2.2356160828779092
8	1.130816839739574	2.387993518125227
9	1.0005369103751691	2.376985396076691
>10	10.415891709696652	54.976451239046206
>50	0.2192847822011338	3.4287649673957437
>100	0.021588277915142177	1.1043643557862493
>500	4.90642442357582E-4	0.0675414400082771
>1k	4.90642442357582E-4	0.21803646069786095
>5k	0.0	0.0
>10k+	2.45321221178791E-4	1.3801636729591658
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21313	1.3655106842097169	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2072	0.13275175421960933	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0369039625629802	0.0
2	0.0	0.0	0.0	0.09527116724158256	0.0
3	0.0	0.0	0.0	0.15434313509413072	0.0
4	0.0	0.0	0.0	0.2298168640857812	0.0
5	0.0	0.0	0.0	0.3826864034525707	0.0
6	0.0	0.0	0.0	0.6391561293893931	0.0
7	0.0	0.0	0.0	0.7861953552262674	0.0
8	0.0	0.0	0.0	1.0764937135124883	0.0
9	0.0	0.0	0.0	1.242753753184248	0.0
10	0.0	0.0	0.0	1.5229291495174295	0.0
11	0.0	0.0	0.0	1.7929175145181213	0.0
12	0.0	0.0	0.0	2.001527414006079	0.0
13	0.0	0.0	0.0	2.1285129240752227	0.0
14	0.0	0.0	0.0	2.2270516296687357	0.0
15	0.0	0.0	0.0	2.300539207897448	0.0
16	0.0	0.0	0.0	2.4059974064715197	0.0
17	0.0	0.0	0.0	2.523949134038267	0.0
18	0.0	0.0	0.0	2.663364103720637	0.0
19	0.0	0.0	0.0	2.7529330961912035	0.0
20	0.0	0.0	0.0	2.8568536296584846	0.0
21	0.0	0.0	0.0	2.9773040630237673	0.0
22	0.0	0.0	0.0	3.117487865259532	0.0
23	0.0	0.0	0.0	3.2654881317881506	0.0
24	0.0	0.0	0.0	3.3861307732917822	0.0
25	0.0	0.0	0.0	3.499277297399808	0.0
26	0.0	0.0	0.0	3.6058887448039734	0.0
27	0.0	0.0	0.0	3.7366543482606445	0.0
28	0.0	0.0	0.0	3.8622303319818965	0.0
29	0.0	0.0	0.0	4.010743153546112	0.0
30	0.0	0.0	0.0	4.189881138487245	0.0
31	0.0	0.0	0.0	4.335895254252925	0.0
32	0.0	0.0	0.0	4.479666941737869	0.0
33	0.0	0.0	0.0	4.621388409080425	0.0
34	0.0	0.0	0.0	4.791172264621914	0.0
35	0.0	0.0	0.0	4.9746669673656205	0.0
36	0.0	0.0	0.0	5.134263791574621	0.0
37	0.0	0.0	0.0	5.303535092080512	0.0
38	0.0	0.0	0.0	5.51547659929985	0.0
39	0.0	0.0	0.0	5.845946458500982	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	25	3.8914503E-5	45.0	31
CGTTTTT	10610	0.0	42.836945	1
TATTGCG	125	0.0	39.6	1
AATGCGG	195	0.0	39.23077	2
GCGATCG	115	0.0	39.130436	9
AACGGGA	810	0.0	38.88889	4
TACGGGA	760	0.0	38.782894	4
GGGCGAT	2430	0.0	38.703705	7
ATAGGGA	3225	0.0	38.372093	4
TACGCGG	100	0.0	38.25	2
TTAGGGA	3095	0.0	38.093697	4
TAGGGAA	2115	0.0	38.08511	5
GACCGAT	965	0.0	38.00518	9
AAGGGAT	2970	0.0	37.5	5
AGGGATC	2190	0.0	37.19178	6
TCACGAC	200	0.0	37.125	25
AGGGCGA	1380	0.0	37.01087	6
TAGGGAT	3185	0.0	36.946625	5
TAAGGGA	2780	0.0	36.906475	4
GGCGATA	665	0.0	36.879696	8
>>END_MODULE