FastQCFastQC Report
Thu 26 May 2016
SRR1547478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547478_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2238474
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144650.6461991517435538No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32840.14670708706020263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG406.8193913E-945.0000041
CCCGTAC207.03472E-445.00000430
ATGCGTA207.03472E-445.00000412
GCGTAAT207.03472E-445.00000420
TCGATAT351.2126111E-745.013
GGTCGTA502.1827873E-1144.99999627
TCCGATC253.8919956E-544.99999614
ACCTACG253.8919956E-544.9999961
TTCGATC253.8919956E-544.99999615
GCGTATT302.1664273E-644.99999614
GCCCGTA253.8919956E-544.99999629
CGGTCTA3450.043.0434831
TAATGCG800.042.1875041
TAGGGCG7000.042.1071435
TATTGCG1550.042.096771
GCGTAAG2250.042.01
CGTTTTT80250.041.271031
CTATACG603.6379788E-1241.2499961
AATGCGG2800.040.982142
ATCGAAC501.0822987E-940.49999635
Produced by FastQC (version 0.11.5)