##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547478_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2238474
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260519443156365	31.0	31.0	33.0	30.0	34.0
2	31.661538619613182	31.0	31.0	34.0	30.0	34.0
3	31.809524702989627	31.0	31.0	34.0	30.0	34.0
4	35.555829551739265	37.0	35.0	37.0	33.0	37.0
5	35.43441737540842	37.0	35.0	37.0	33.0	37.0
6	35.48583275928154	37.0	35.0	37.0	33.0	37.0
7	35.89782950349211	37.0	35.0	37.0	35.0	37.0
8	35.929726233139185	37.0	35.0	37.0	35.0	37.0
9	37.66623512267732	39.0	37.0	39.0	35.0	39.0
10	36.97241468965018	39.0	37.0	39.0	32.0	39.0
11	36.76741387212896	39.0	37.0	39.0	32.0	39.0
12	36.28384292156174	38.0	35.0	39.0	32.0	39.0
13	36.080829172016294	38.0	35.0	39.0	31.0	39.0
14	37.1874017746018	39.0	35.0	41.0	32.0	41.0
15	37.35226498051798	39.0	35.0	41.0	32.0	41.0
16	37.417343690389075	39.0	35.0	41.0	32.0	41.0
17	37.3317992525265	39.0	35.0	41.0	32.0	41.0
18	37.238399016472826	39.0	36.0	41.0	32.0	41.0
19	37.170471490846	39.0	35.0	41.0	32.0	41.0
20	37.036100486313444	39.0	35.0	41.0	32.0	41.0
21	36.986418872857136	39.0	35.0	41.0	31.0	41.0
22	36.87071594309337	39.0	35.0	41.0	31.0	41.0
23	36.7998305988812	38.0	35.0	41.0	31.0	41.0
24	36.713972107784144	38.0	35.0	40.0	31.0	41.0
25	36.60968901135327	38.0	35.0	40.0	31.0	41.0
26	36.472181941805	38.0	35.0	40.0	31.0	41.0
27	36.376100861569086	38.0	35.0	40.0	30.0	41.0
28	36.28783179969926	38.0	35.0	40.0	30.0	41.0
29	36.334694975237596	38.0	35.0	40.0	30.0	41.0
30	36.303627828601094	38.0	35.0	40.0	30.0	41.0
31	36.191972745718736	38.0	35.0	40.0	30.0	41.0
32	36.09051255453492	38.0	35.0	40.0	30.0	41.0
33	35.978724345245915	38.0	35.0	40.0	30.0	41.0
34	35.843032351503744	38.0	35.0	40.0	29.0	41.0
35	35.73651693073049	38.0	35.0	40.0	29.0	41.0
36	35.645925304470815	38.0	35.0	40.0	27.0	41.0
37	35.593042402994186	38.0	34.0	40.0	27.0	41.0
38	35.49415047929974	38.0	34.0	40.0	27.0	41.0
39	35.41230722358178	38.0	34.0	40.0	27.0	41.0
40	35.277554709145605	38.0	34.0	40.0	26.0	41.0
41	35.188326064988914	38.0	34.0	40.0	26.0	41.0
42	35.138612733496124	38.0	34.0	40.0	26.0	41.0
43	35.097369904676135	38.0	34.0	40.0	26.0	41.0
44	34.96545861153625	38.0	34.0	40.0	25.0	41.0
45	34.83976181988265	38.0	34.0	40.0	24.0	41.0
46	34.78910364828897	37.0	34.0	40.0	24.0	41.0
47	34.72816793940872	37.0	34.0	40.0	24.0	41.0
48	34.664443276982446	37.0	34.0	40.0	24.0	41.0
49	34.594574250136475	37.0	34.0	40.0	24.0	41.0
50	34.473172795395435	37.0	33.0	40.0	24.0	41.0
51	33.91221430313687	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	3.0
13	6.0
14	25.0
15	45.0
16	138.0
17	431.0
18	957.0
19	1886.0
20	3216.0
21	5285.0
22	8145.0
23	11938.0
24	17472.0
25	24397.0
26	32000.0
27	37927.0
28	42375.0
29	49406.0
30	58110.0
31	70878.0
32	86337.0
33	108931.0
34	167370.0
35	238023.0
36	175141.0
37	217219.0
38	316930.0
39	563536.0
40	344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.593421232500354	25.814103715298902	26.643999438903464	13.948475613297273
2	27.95015711596382	28.28726176850837	30.27115794063277	13.49142317489504
3	28.027039849468878	26.091390831432488	32.975098214229874	12.906471104868764
4	25.4425112822396	28.894907870272334	31.21278156458373	14.449799282904335
5	23.020995553220633	33.336907196599114	29.73119187446448	13.91090537571578
6	21.626161393878153	41.366752528731624	26.47039903076828	10.536687046621939
7	88.84905520457241	3.436626916372493	6.434964176488089	1.2793537025670165
8	88.84114803209687	3.097824678776702	5.761737683797087	2.299289605329345
9	82.89790276768906	6.7385638609159635	7.728166599209997	2.6353667721849794
10	33.14744777022203	41.623311237923694	13.755218957200308	11.474022034653965
11	33.97443972992315	23.91213835854247	25.564067306566884	16.54935460496749
12	33.33194846131784	21.527612114324313	26.71873785444906	18.421701569908784
13	23.63127737914311	30.586908760164288	27.539162840399307	18.242651020293287
14	18.340351507321508	33.231790943294406	28.419807422377925	20.008050127006165
15	17.01458225559019	27.549571717160887	37.08906156604901	18.346784461199906
16	20.837275751248395	24.808775978635445	35.37878930021077	18.97515896990539
17	19.982943737564074	26.16385090914614	27.89360072978288	25.959604623506905
18	20.528940697993363	26.24037625632462	32.154762574861266	21.075920470820748
19	24.002959158784066	27.81032971569024	27.623863399798253	20.562847725727437
20	25.62433157588607	26.65516776160902	28.70678864261993	19.013712019884977
21	22.807144510054616	28.95664635818866	29.40150298819642	18.8347061435603
22	23.579411688498503	25.64505998282759	27.617251752756566	23.158276575917345
23	20.096949975742405	29.583725341460298	27.672825326539417	22.64649935625788
24	19.89270369010317	26.85771646219701	34.70793049193334	18.541649355766474
25	19.329820225743074	28.81958870194606	30.846013846933225	21.004577225377645
26	19.114673657143214	33.1552209228251	26.86982292400984	20.860282496021842
27	19.335672426840787	32.28672747595013	28.79135518214641	19.58624491506267
28	17.572953717577242	30.258962132238302	32.99466511561001	19.173419034574447
29	18.22384356485713	27.040206855205824	31.785091093307315	22.95085848662973
30	19.460400254816452	31.634676123108868	29.35441733966979	19.55050628240489
31	23.132678780276205	29.833672403610674	26.968684916599432	20.064963899513685
32	23.699850880555235	29.204717142124505	27.338981824224895	19.75645015309537
33	23.40460510151112	29.407935942074825	25.936776571896747	21.250682384517308
34	20.033469229484012	28.453893143275284	28.822894525466904	22.6897431017738
35	20.74180892876129	27.666571065824307	29.091023616981925	22.500596388432477
36	24.31183029152896	28.498119701189296	27.490736993148012	19.699313014133736
37	19.737598024368385	31.07290949101933	29.480664059533414	19.70882842507887
38	20.2090799357062	30.900068528828122	27.532059787158573	21.358791748307105
39	20.948110185778347	29.565319945641537	28.19139288640386	21.29517698217625
40	22.443235883016737	27.754890161779855	27.513073638559128	22.288800316644284
41	18.38788388875636	26.418265300378742	30.495194494106254	24.69865631675865
42	20.615160149280268	27.265047527914106	28.167716042268083	23.952076280537547
43	21.040449877907896	27.497840046388745	28.2630041715919	23.19870590411146
44	19.715082685793984	29.06104783884021	27.968830551527514	23.255038923838292
45	18.531687211913116	32.19854239986705	26.55912018634123	22.7106502018786
46	21.355619944658727	30.781326921822632	26.931427392053692	20.93162574146494
47	20.120805513041475	27.861882693299094	29.450241548483476	22.567070245175955
48	20.244773895073163	27.29895455564818	31.116689316025116	21.339582233253545
49	21.03428496377443	26.76971007927722	30.134770383752503	22.061234573195847
50	19.923260221025572	28.30231666751546	29.97653758766016	21.7978855237988
51	18.527889982193226	28.494233124887757	28.4794462656256	24.498430627293416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1545.0
1	2495.0
2	3445.0
3	12585.5
4	21726.0
5	15186.5
6	8647.0
7	9005.0
8	9363.0
9	10456.5
10	11550.0
11	12191.0
12	12832.0
13	12776.0
14	12720.0
15	12268.0
16	11816.0
17	11990.5
18	12165.0
19	12342.5
20	12520.0
21	12180.0
22	11840.0
23	13522.5
24	15205.0
25	16616.0
26	21810.5
27	25594.0
28	32120.5
29	38647.0
30	41963.0
31	45279.0
32	53047.0
33	60815.0
34	66823.5
35	72832.0
36	75564.0
37	78296.0
38	89792.0
39	101288.0
40	119511.0
41	137734.0
42	155393.0
43	173052.0
44	182284.0
45	191516.0
46	195247.0
47	198978.0
48	200666.5
49	202355.0
50	185617.5
51	168880.0
52	150306.0
53	131732.0
54	119511.0
55	107290.0
56	97410.0
57	87530.0
58	79224.0
59	70918.0
60	65654.0
61	60390.0
62	51876.5
63	43363.0
64	36056.5
65	28750.0
66	23909.0
67	19068.0
68	15574.0
69	12080.0
70	9455.0
71	6830.0
72	6110.5
73	5391.0
74	4031.0
75	2037.5
76	1404.0
77	1286.0
78	1168.0
79	896.0
80	624.0
81	395.5
82	167.0
83	203.0
84	239.0
85	176.5
86	114.0
87	88.5
88	63.0
89	36.5
90	10.0
91	5.5
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2238474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.22014702023325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10269717446818	15.011453223700347
2	9.230435880199785	3.54820669356552
3	3.2419495751958722	1.8693224240234223
4	1.6138817696032564	1.2407617954019519
5	0.9235212415332434	0.887510701928864
6	0.6114137258834037	0.7050877021000568
7	0.4406532538297134	0.5928594226485877
8	0.33228271059460096	0.5109218039927879
9	0.26104234773251717	0.4515545071733249
>10	2.60602464065868	11.664699084240253
>50	1.1952540048654907	16.86081428280069
>100	1.432616998686894	44.434076758904695
>500	0.004936006049069404	0.618241132079116
>1k	0.0030556227922810594	0.9510116237776742
>5k	0.0	0.0
>10k+	2.3504790709854298E-4	0.6534788436627266
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14465	0.6461991517435538	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3284	0.14670708706020263	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.934658164445957E-5	0.0	0.0	0.08246689485783619	0.0
2	8.934658164445957E-5	0.0	0.0	0.2556205700847988	0.0
3	8.934658164445957E-5	0.0	0.0	0.38897034318915474	0.0
4	8.934658164445957E-5	0.0	0.0	0.7380474376740583	0.0
5	8.934658164445957E-5	0.0	0.0	1.1453785033911494	0.0
6	8.934658164445957E-5	0.0	0.0	1.775539943729523	0.0
7	8.934658164445957E-5	0.0	0.0	2.024414846900165	0.0
8	8.934658164445957E-5	0.0	0.0	2.730699574799618	0.0
9	8.934658164445957E-5	0.0	0.0	2.9557636139620116	0.0
10	8.934658164445957E-5	0.0	0.0	3.426932812264069	0.0
11	8.934658164445957E-5	0.0	0.0	4.017290350479835	0.0
12	8.934658164445957E-5	0.0	0.0	4.488906281690116	0.0
13	8.934658164445957E-5	0.0	0.0	4.701506472713107	0.0
14	8.934658164445957E-5	0.0	0.0	4.791299787265789	0.0
15	8.934658164445957E-5	0.0	0.0	4.9213437368493	0.0
16	8.934658164445957E-5	0.0	0.0	5.182637814868522	0.0
17	8.934658164445957E-5	0.0	0.0	5.490749501669441	0.0
18	8.934658164445957E-5	0.0	0.0	5.851843711385524	0.0
19	8.934658164445957E-5	0.0	0.0	6.04751272518689	0.0
20	8.934658164445957E-5	0.0	0.0	6.257700558505482	0.0
21	8.934658164445957E-5	0.0	0.0	6.541509975099108	0.0
22	8.934658164445957E-5	0.0	0.0	6.837515200087203	0.0
23	8.934658164445957E-5	0.0	0.0	7.162379370946457	0.0
24	8.934658164445957E-5	0.0	0.0	7.392759531716696	0.0
25	1.3401987246668937E-4	0.0	0.0	7.599775561386909	0.0
26	1.3401987246668937E-4	0.0	0.0	7.793836336718675	0.0
27	1.3401987246668937E-4	0.0	0.0	7.995000165291176	0.0
28	1.3401987246668937E-4	0.0	0.0	8.207779049477457	0.0
29	1.3401987246668937E-4	0.0	0.0	8.444190104508696	0.0
30	1.3401987246668937E-4	0.0	0.0	8.70070414040994	0.0
31	1.7869316328891915E-4	0.0	0.0	8.944441615136025	0.0
32	1.7869316328891915E-4	0.0	0.0	9.167986762410463	0.0
33	1.7869316328891915E-4	0.0	0.0	9.39711607103768	0.0
34	1.7869316328891915E-4	0.0	0.0	9.629461856604097	0.0
35	1.7869316328891915E-4	0.0	0.0	9.888879656408786	0.0
36	1.7869316328891915E-4	0.0	0.0	10.123950512715359	0.0
37	1.7869316328891915E-4	0.0	0.0	10.38001781570838	0.0
38	1.7869316328891915E-4	0.0	0.0	10.636219138573868	0.0
39	1.7869316328891915E-4	0.0	0.0	10.91556122608527	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	40	6.8193913E-9	45.000004	1
CCCGTAC	20	7.03472E-4	45.000004	30
ATGCGTA	20	7.03472E-4	45.000004	12
GCGTAAT	20	7.03472E-4	45.000004	20
TCGATAT	35	1.2126111E-7	45.0	13
GGTCGTA	50	2.1827873E-11	44.999996	27
TCCGATC	25	3.8919956E-5	44.999996	14
ACCTACG	25	3.8919956E-5	44.999996	1
TTCGATC	25	3.8919956E-5	44.999996	15
GCGTATT	30	2.1664273E-6	44.999996	14
GCCCGTA	25	3.8919956E-5	44.999996	29
CGGTCTA	345	0.0	43.04348	31
TAATGCG	80	0.0	42.187504	1
TAGGGCG	700	0.0	42.107143	5
TATTGCG	155	0.0	42.09677	1
GCGTAAG	225	0.0	42.0	1
CGTTTTT	8025	0.0	41.27103	1
CTATACG	60	3.6379788E-12	41.249996	1
AATGCGG	280	0.0	40.98214	2
ATCGAAC	50	1.0822987E-9	40.499996	35
>>END_MODULE