FastQCFastQC Report
Thu 26 May 2016
SRR1547477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547477_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3102450
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131540.4239874937549356No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32500.10475591870940709No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT302.1666983E-645.00000444
CGGTCTA8800.042.18750431
CGACGGT8850.041.44067828
TCACGAC9000.041.025
TTACGCG556.184564E-1140.9090921
ACGGTCT9100.040.79670330
CGTTTTT81600.040.615811
CACGACG9100.040.5494526
AATCGGC501.0822987E-940.532
CACGACC4800.040.31250427
TCTCACG9650.039.17098223
GTTTGCG1900.039.0789451
TACGGGA14100.038.936174
ACGGGAT17700.038.6440665
ACGACGG9750.037.84615327
AGGGCGA26000.037.8173076
TAAGGGA57000.037.7763144
ACTAACC10000.037.57514
TAACGGG6350.037.5590553
TTAGGGA61200.037.5367624
Produced by FastQC (version 0.11.5)