##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547477_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3102450
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248623184902254	31.0	31.0	33.0	30.0	34.0
2	31.67512159744718	31.0	31.0	34.0	30.0	34.0
3	31.830854324807813	31.0	31.0	34.0	30.0	34.0
4	35.55954713210527	37.0	35.0	37.0	33.0	37.0
5	35.424959951006464	37.0	35.0	37.0	33.0	37.0
6	35.47606085513062	37.0	35.0	37.0	33.0	37.0
7	35.905973343647766	37.0	35.0	37.0	35.0	37.0
8	35.93952779255105	37.0	35.0	37.0	35.0	37.0
9	37.66009798707473	39.0	37.0	39.0	35.0	39.0
10	36.97839836258441	39.0	37.0	39.0	32.0	39.0
11	36.78993569598221	39.0	37.0	39.0	32.0	39.0
12	36.347672968138085	38.0	35.0	39.0	32.0	39.0
13	36.19760125062451	38.0	35.0	39.0	32.0	39.0
14	37.25092942674338	39.0	36.0	41.0	32.0	41.0
15	37.39839320536995	39.0	36.0	41.0	32.0	41.0
16	37.44805299037857	39.0	36.0	41.0	32.0	41.0
17	37.36823381521056	39.0	35.0	41.0	32.0	41.0
18	37.28627665232317	39.0	36.0	41.0	32.0	41.0
19	37.247903431159244	39.0	36.0	41.0	32.0	41.0
20	37.143285145610726	39.0	35.0	41.0	31.0	41.0
21	37.08756531128624	39.0	35.0	41.0	31.0	41.0
22	37.00838724234073	39.0	35.0	41.0	31.0	41.0
23	36.92497510032394	39.0	35.0	41.0	31.0	41.0
24	36.855236023143	39.0	35.0	41.0	31.0	41.0
25	36.74828635433287	38.0	35.0	41.0	31.0	41.0
26	36.60415929346162	38.0	35.0	40.0	31.0	41.0
27	36.509716514367675	38.0	35.0	40.0	30.0	41.0
28	36.41806475527406	38.0	35.0	40.0	30.0	41.0
29	36.46350948443972	38.0	35.0	40.0	30.0	41.0
30	36.417239278634625	38.0	35.0	40.0	30.0	41.0
31	36.322700124095476	38.0	35.0	40.0	30.0	41.0
32	36.28257538397073	38.0	35.0	40.0	30.0	41.0
33	36.212697061999386	38.0	35.0	41.0	30.0	41.0
34	36.09450079775661	38.0	35.0	41.0	29.0	41.0
35	36.006782381666106	38.0	35.0	41.0	29.0	41.0
36	35.96670051088656	38.0	35.0	41.0	29.0	41.0
37	35.92622540250447	38.0	35.0	41.0	29.0	41.0
38	35.83312414382182	38.0	35.0	41.0	28.0	41.0
39	35.738668794017634	38.0	35.0	40.0	28.0	41.0
40	35.65089042530903	38.0	35.0	40.0	27.0	41.0
41	35.55487115021999	38.0	34.0	40.0	27.0	41.0
42	35.5177566117101	38.0	34.0	40.0	27.0	41.0
43	35.48585827330013	38.0	34.0	40.0	27.0	41.0
44	35.37127850569711	38.0	34.0	40.0	27.0	41.0
45	35.24127576592693	38.0	34.0	40.0	26.0	41.0
46	35.18884268884269	38.0	34.0	40.0	26.0	41.0
47	35.13762897065223	38.0	34.0	40.0	26.0	41.0
48	35.082638237521955	38.0	34.0	40.0	26.0	41.0
49	35.009551805830874	38.0	34.0	40.0	26.0	41.0
50	34.89717997066834	37.0	34.0	40.0	26.0	41.0
51	34.325049235281796	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	0.0
12	8.0
13	9.0
14	18.0
15	98.0
16	214.0
17	608.0
18	1260.0
19	2423.0
20	4223.0
21	6699.0
22	10061.0
23	15124.0
24	22031.0
25	30560.0
26	40578.0
27	48521.0
28	55948.0
29	66198.0
30	79060.0
31	95264.0
32	116786.0
33	147443.0
34	217492.0
35	287659.0
36	244790.0
37	315232.0
38	468230.0
39	825492.0
40	416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.872616802849365	24.890779867524053	30.238037679898145	12.998565649728441
2	29.549098293284338	26.855324018114718	29.751518960821286	13.844058727779657
3	29.24392012764106	25.554738996599465	30.897097455236988	14.304243420522491
4	25.382488033650823	28.456800270753757	31.092233557349836	15.068478138245581
5	23.02177311479637	33.31438057019452	29.211590839497813	14.452255475511288
6	22.243194894357686	41.42194072426631	26.363938177891665	9.970926203484343
7	90.80771648213508	3.090525230060114	4.996857322438718	1.1049009653660817
8	91.09468323421812	2.3719640928943257	4.828377572563619	1.7049751003239375
9	84.5780592757337	6.7038308433657265	6.372060790665442	2.346049090235137
10	30.369256555303064	47.87213331399378	11.57913906751116	10.179471063191993
11	33.58955019420136	24.409096037003014	25.972151041918483	16.029202726877145
12	32.0740060274944	22.64381376009283	26.524198617221874	18.7579815951909
13	25.494399587422844	27.7805282921562	28.218601427903756	18.506470692517205
14	20.215603796999147	30.67066350787281	29.245467291978922	19.868265403149124
15	19.052974262276585	27.015036503408595	35.189285886960306	18.74270334735451
16	23.549066060693967	25.588164192815356	32.6121613563474	18.250608390143274
17	23.04849393221486	25.09110541668681	28.220922174410546	23.639478476687778
18	22.94928201904946	25.7017840738771	30.995020064787504	20.353913842285934
19	25.049525375106768	26.678141468839144	27.61162951860626	20.660703637447824
20	26.342439040113458	27.61881738625925	27.22577317926155	18.812970394365745
21	24.761785040854807	26.64336250382762	30.03010523940757	18.564747215910007
22	24.392109461876903	24.014891456751922	29.483698367419297	22.10930071395188
23	20.910377282470304	27.58784186691163	29.51947654273236	21.982304307885702
24	20.50489129558897	27.00839658979194	32.342503505294204	20.144208609324888
25	21.079114893068382	28.06842978936002	30.19462038066689	20.657834936904703
26	21.05300649486696	30.01334429241406	27.63084014246805	21.30280907025093
27	20.280391303647118	29.821560379699914	29.065383809569855	20.832664507083113
28	18.616448290866895	28.89851568921336	31.861303163628747	20.623732856290996
29	20.177891666263758	26.524617640896707	31.083853083853086	22.213637608986446
30	21.406469080887685	28.020113136392204	29.731921545875036	20.841496236845074
31	24.09186288256056	27.519960031587935	28.22794887911167	20.160228206739834
32	24.598655900981484	26.42927363857596	29.007397379490403	19.96467308095215
33	24.246643781527503	26.524649873487082	28.47349675256652	20.755209592418893
34	20.754178149526986	27.667972086576736	29.479475898080548	22.098373865815727
35	20.758819642540573	27.292720269464454	30.07964673080952	21.868813357185452
36	23.97856532740254	27.04182178600783	28.115650534255188	20.863962352334443
37	21.092523650663185	30.08702799400474	28.500249802575382	20.320198552756693
38	20.978807071830328	29.2807619784364	27.87806411062225	21.862366839111026
39	20.698544698544698	28.541314122709473	29.187029605634258	21.57311157311157
40	22.993472900449643	26.92755725313865	28.577188995793644	21.50178085061806
41	18.84462279811117	26.81190671888346	29.29919902012925	25.044271462876118
42	21.035762059017873	26.80810327321955	28.22269496688101	23.93343970088156
43	21.116569163080793	26.921110735064225	28.930232558139537	23.032087543715452
44	20.97761446598656	27.573852922690133	29.454624570903643	21.99390804041967
45	20.421376653934793	28.93410046898419	27.54020209834163	23.104320778739385
46	21.74201034666151	28.171767474093055	28.270785991716224	21.81543618752921
47	20.677496817031702	27.583683862753634	29.552643878225272	22.186175441989395
48	21.035439733114153	27.491949910554563	29.77566117101001	21.69694918532128
49	21.562668213831003	26.481909458653647	29.852922690131994	22.102499637383357
50	20.488452674499186	28.47942754919499	29.190542957984817	21.841576818321006
51	19.75354961401473	28.164676304211188	28.83405050846911	23.247723573304967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1383.0
1	2784.5
2	4186.0
3	13017.0
4	21848.0
5	15600.5
6	9353.0
7	9784.5
8	10216.0
9	10951.5
10	11687.0
11	12237.5
12	12788.0
13	12751.0
14	12714.0
15	12642.5
16	12571.0
17	11944.0
18	11317.0
19	12254.5
20	13192.0
21	13810.0
22	14428.0
23	16580.5
24	18733.0
25	21572.0
26	29971.0
27	35531.0
28	43696.5
29	51862.0
30	57766.0
31	63670.0
32	74909.5
33	86149.0
34	97888.0
35	109627.0
36	116445.0
37	123263.0
38	137273.0
39	151283.0
40	167672.0
41	184061.0
42	203018.5
43	221976.0
44	230057.5
45	238139.0
46	243745.0
47	249351.0
48	253196.5
49	257042.0
50	244344.5
51	231647.0
52	218414.5
53	205182.0
54	186695.5
55	168209.0
56	156107.5
57	144006.0
58	133255.5
59	122505.0
60	105913.0
61	89321.0
62	78349.0
63	67377.0
64	57036.5
65	46696.0
66	38211.5
67	29727.0
68	24385.5
69	19044.0
70	14902.5
71	10761.0
72	8856.5
73	6952.0
74	5669.5
75	3916.0
76	3445.0
77	2264.0
78	1083.0
79	961.0
80	839.0
81	598.5
82	358.0
83	218.5
84	79.0
85	47.0
86	15.0
87	19.5
88	24.0
89	17.5
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3102450.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.84615276016043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.00503574620228	13.30930901002459
2	8.399230320912404	2.8298943410772335
3	2.8263674786279385	1.4284025490394716
4	1.3140858461352232	0.8854916361583458
5	0.7655731590757455	0.6448481193434304
6	0.5300938848690377	0.5358025537038423
7	0.37218082554969273	0.438887052912892
8	0.295256164458334	0.39791443598753173
9	0.2262382045742607	0.34301190189981795
>10	2.854108275847823	11.981189804257744
>50	1.6294305351055514	20.139039640326807
>100	1.7760131140552107	45.727859014928505
>500	0.0048382155958418644	0.5564205118457491
>1k	0.001354700366835722	0.35308104196926604
>5k	0.0	0.0
>10k+	1.9352862383367457E-4	0.4288483865248131
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13154	0.4239874937549356	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3250	0.10475591870940709	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.446518074425051E-5	0.0	0.0	0.03983948169994682	0.0
2	6.446518074425051E-5	0.0	0.0	0.14565907589163404	0.0
3	6.446518074425051E-5	0.0	0.0	0.22504794597817854	0.0
4	6.446518074425051E-5	0.0	0.0	0.4181211623072088	0.0
5	6.446518074425051E-5	0.0	0.0	0.6929362278199488	0.0
6	6.446518074425051E-5	0.0	0.0	1.0442069976953698	0.0
7	6.446518074425051E-5	0.0	0.0	1.207174974616835	0.0
8	6.446518074425051E-5	0.0	0.0	1.627906976744186	0.0
9	6.446518074425051E-5	0.0	0.0	1.7703750261889797	0.0
10	6.446518074425051E-5	0.0	0.0	2.0510886557398185	0.0
11	6.446518074425051E-5	0.0	0.0	2.3976857000112815	0.0
12	6.446518074425051E-5	0.0	0.0	2.690937807216877	0.0
13	6.446518074425051E-5	0.0	0.0	2.815420071234025	0.0
14	6.446518074425051E-5	0.0	0.0	2.871794871794872	0.0
15	6.446518074425051E-5	0.0	0.0	2.9565343518831892	0.0
16	6.446518074425051E-5	0.0	0.0	3.1220487034440523	0.0
17	6.446518074425051E-5	0.0	0.0	3.3119631259166145	0.0
18	6.446518074425051E-5	0.0	0.0	3.5407500523779594	0.0
19	6.446518074425051E-5	0.0	0.0	3.6698093442279487	0.0
20	9.669777111637576E-5	0.0	0.0	3.8133088365646506	0.0
21	9.669777111637576E-5	0.0	0.0	3.995068413673065	0.0
22	9.669777111637576E-5	0.0	0.0	4.205708391754904	0.0
23	9.669777111637576E-5	0.0	0.0	4.411642411642411	0.0
24	9.669777111637576E-5	0.0	0.0	4.577285693564764	0.0
25	9.669777111637576E-5	0.0	0.0	4.721977791745234	0.0
26	1.2893036148850103E-4	0.0	0.0	4.8645103063707715	0.0
27	1.2893036148850103E-4	0.0	0.0	5.018904414253251	0.0
28	1.2893036148850103E-4	0.0	0.0	5.176392850811456	0.0
29	1.2893036148850103E-4	0.0	0.0	5.352511724604748	0.0
30	1.2893036148850103E-4	0.0	0.0	5.5664716594949155	0.0
31	1.2893036148850103E-4	0.0	0.0	5.753420683653242	0.0
32	1.2893036148850103E-4	0.0	0.0	5.920256571419362	0.0
33	1.2893036148850103E-4	0.0	0.0	6.10191945075666	0.0
34	1.2893036148850103E-4	0.0	0.0	6.298280391303647	0.0
35	1.2893036148850103E-4	0.0	0.0	6.510789859627069	0.0
36	1.2893036148850103E-4	0.0	0.0	6.7016390272204225	0.0
37	1.2893036148850103E-4	0.0	0.0	6.898870247707457	0.0
38	1.2893036148850103E-4	0.0	0.0	7.115924511273349	0.0
39	1.2893036148850103E-4	0.0	0.0	7.436155296620413	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	30	2.1666983E-6	45.000004	44
CGGTCTA	880	0.0	42.187504	31
CGACGGT	885	0.0	41.440678	28
TCACGAC	900	0.0	41.0	25
TTACGCG	55	6.184564E-11	40.909092	1
ACGGTCT	910	0.0	40.796703	30
CGTTTTT	8160	0.0	40.61581	1
CACGACG	910	0.0	40.54945	26
AATCGGC	50	1.0822987E-9	40.5	32
CACGACC	480	0.0	40.312504	27
TCTCACG	965	0.0	39.170982	23
GTTTGCG	190	0.0	39.078945	1
TACGGGA	1410	0.0	38.93617	4
ACGGGAT	1770	0.0	38.644066	5
ACGACGG	975	0.0	37.846153	27
AGGGCGA	2600	0.0	37.817307	6
TAAGGGA	5700	0.0	37.776314	4
ACTAACC	1000	0.0	37.575	14
TAACGGG	635	0.0	37.559055	3
TTAGGGA	6120	0.0	37.536762	4
>>END_MODULE