FastQCFastQC Report
Thu 26 May 2016
SRR1547476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547476_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3148655
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523131.6614395670532338No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT44090.14002804372025515No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT42230.13412075949889715No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC35020.11122209324298789No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG33850.10750622091019817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG551.8189894E-1244.9999961
CGTTTTT181050.043.3347131
GCGATAT2450.042.24499
ATACCGG501.0822987E-940.52
TAGGGCG12100.040.351245
AGGGCGA30500.040.2049186
TAATGCG1400.040.178571
ATTCGGC451.9301297E-840.017
TATACGA451.9301297E-840.026
CGACGGT5750.039.91304428
CGGTCTA5750.039.91304431
CGTAAGG6450.039.767442
TATAGCG1250.039.61
GGGCGAT57150.039.527567
CGGCATA403.460773E-739.37500425
ATTACGT403.460773E-739.37500412
TCGGAAC403.460773E-739.37500440
CGTACCG403.460773E-739.37500445
ACGTACC403.460773E-739.37500416
CGAGGGA20850.039.064754