##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547476_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3148655
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226277569311343	31.0	31.0	33.0	30.0	34.0
2	31.620779983834367	31.0	31.0	34.0	30.0	34.0
3	31.681920057929496	31.0	31.0	34.0	30.0	34.0
4	35.494021733089205	37.0	35.0	37.0	33.0	37.0
5	35.38284982000251	37.0	35.0	37.0	33.0	37.0
6	35.431889489321634	37.0	35.0	37.0	33.0	37.0
7	35.894922435135	37.0	35.0	37.0	35.0	37.0
8	35.93330993710013	37.0	35.0	37.0	35.0	37.0
9	37.65031735772894	39.0	37.0	39.0	35.0	39.0
10	36.95593007172904	39.0	37.0	39.0	32.0	39.0
11	36.73678062537814	39.0	35.0	39.0	32.0	39.0
12	36.42622738915505	38.0	35.0	39.0	32.0	39.0
13	36.37437540791227	39.0	35.0	39.0	32.0	39.0
14	37.50917963384366	39.0	36.0	41.0	32.0	41.0
15	37.601563524743106	39.0	36.0	41.0	32.0	41.0
16	37.62890504040614	40.0	36.0	41.0	32.0	41.0
17	37.55334388810461	39.0	36.0	41.0	32.0	41.0
18	37.41987801140487	39.0	36.0	41.0	32.0	41.0
19	37.32743790602654	39.0	36.0	41.0	32.0	41.0
20	37.17551557728618	39.0	35.0	41.0	32.0	41.0
21	37.110793021147124	39.0	35.0	41.0	32.0	41.0
22	37.00675018380864	39.0	35.0	41.0	31.0	41.0
23	36.89330079033746	39.0	35.0	41.0	31.0	41.0
24	36.7910793656339	38.0	35.0	40.0	31.0	41.0
25	36.66629497356808	38.0	35.0	40.0	31.0	41.0
26	36.50897796043073	38.0	35.0	40.0	31.0	41.0
27	36.412223631995246	38.0	35.0	40.0	31.0	41.0
28	36.28226560229685	38.0	35.0	40.0	30.0	41.0
29	36.287250905545385	38.0	35.0	40.0	30.0	41.0
30	36.18390868481939	38.0	35.0	40.0	30.0	41.0
31	36.06648997746657	38.0	35.0	40.0	30.0	41.0
32	35.88988028221574	38.0	35.0	40.0	30.0	41.0
33	35.7426266135858	38.0	35.0	40.0	29.0	41.0
34	35.54323766814719	38.0	35.0	40.0	27.0	41.0
35	35.38261416382551	38.0	34.0	40.0	26.0	41.0
36	35.32914149057296	38.0	34.0	40.0	26.0	41.0
37	35.27141874864029	38.0	34.0	40.0	26.0	41.0
38	35.168661222013846	38.0	34.0	40.0	25.0	41.0
39	35.066598912869146	38.0	34.0	40.0	25.0	41.0
40	34.96408625270155	38.0	34.0	40.0	24.0	41.0
41	34.84870111206214	38.0	34.0	40.0	24.0	41.0
42	34.77575250384688	38.0	34.0	40.0	24.0	41.0
43	34.719309991091436	38.0	34.0	40.0	24.0	41.0
44	34.57798393282211	37.0	33.0	40.0	23.0	41.0
45	34.44915241587281	37.0	33.0	40.0	23.0	41.0
46	34.393145962323594	37.0	33.0	40.0	23.0	41.0
47	34.322091496210284	37.0	33.0	40.0	23.0	41.0
48	34.23801178598481	37.0	33.0	40.0	23.0	41.0
49	34.15354683190124	37.0	33.0	40.0	23.0	41.0
50	34.02869796786247	36.0	33.0	40.0	23.0	41.0
51	33.518195229391594	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	6.0
13	7.0
14	25.0
15	79.0
16	229.0
17	566.0
18	1230.0
19	2458.0
20	4372.0
21	7212.0
22	11446.0
23	17614.0
24	26761.0
25	39350.0
26	50546.0
27	57302.0
28	62687.0
29	70623.0
30	83751.0
31	100246.0
32	122003.0
33	153774.0
34	221307.0
35	309064.0
36	302514.0
37	329184.0
38	440122.0
39	733778.0
40	394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.551249660569354	25.002548707305184	28.03965502730531	15.406546604820154
2	32.437374053365644	26.457328605388653	27.802125034340058	13.303172306905648
3	27.110210550219062	25.762841594268025	33.67113894662959	13.455808908883316
4	24.440054562980066	28.448559781875115	32.83808483304776	14.273300822097054
5	22.00463372455858	32.16027160803581	31.704934329102425	14.13016033830318
6	20.6868964684921	40.848235198838864	28.142746664845784	10.322121667823245
7	85.39458911821079	3.0083956483006236	9.42170545836238	2.175309775126205
8	84.66481719972496	2.8671924996546148	9.142887995032801	3.325102305587624
9	78.71615022922485	6.153865698210823	11.74463381983736	3.3853502527269583
10	31.656500950405807	38.638593304125095	17.201567018298288	12.503338727170807
11	29.38540424403436	25.774624403118157	27.566373578559734	17.27359777428775
12	26.260069775824917	24.714616240902863	29.420847949362503	19.604466033909716
13	22.347986680026867	28.653059798548902	31.451111665139557	17.54784185628467
14	17.964210115112643	32.271271384130685	30.75973709409256	19.004781406664115
15	17.01269272117777	29.673146152881152	35.73440087910552	17.579760246835555
16	19.60163943016939	28.498803457349247	34.3627675944173	17.53678951806406
17	18.03182628773238	30.449382355323145	29.767567421645115	21.751223935299357
18	19.50455035562804	29.93189155369515	31.583104531935064	18.980453558741747
19	21.57232215025146	32.4351191222919	27.321634158077018	18.670924569379626
20	23.05162045381282	30.965856849988327	27.452166083613477	18.530356612585376
21	21.558887842586756	32.16446387425742	28.55352523537828	17.72312304777754
22	21.01802198081403	29.313087651711605	28.35077199629683	21.318118371177537
23	18.69229877519131	32.81553552231032	27.640754544400703	20.85141115809766
24	18.19386372911608	30.78416022079269	32.34876479004527	18.673211260045957
25	18.402079618122656	32.20597366176987	29.71519585346759	19.676750866639882
26	18.076512034503622	34.288545426539265	28.719627904613237	18.915314634343872
27	18.485893182962247	33.03858314105547	28.783020051418777	19.692503624563503
28	17.601388529387947	33.028610628982854	31.00714432035266	18.362856521276544
29	18.223336631037697	30.30567019886269	30.705872825063402	20.765120345036216
30	19.31913785409961	31.42471944369898	30.418924906031304	18.837217796170112
31	20.98769156989254	31.315085330085385	28.16104019017644	19.536182909845632
32	21.34727367717327	30.764469273388162	29.342179438522166	18.546077610916406
33	20.933604983715266	30.228017994985162	29.08848381292965	19.749893208369922
34	19.142427480940274	30.586964910414128	28.976912364168193	21.293695244477405
35	18.011944782772325	30.330029806377645	30.024439006496422	21.633586404353604
36	19.856002007206254	30.620439521001824	28.258383341458497	21.26517513033343
37	18.468298368668528	32.26530058072415	28.84193409566942	20.424466954937902
38	18.033827142065423	32.35067036560055	28.019964079900785	21.595538412433246
39	18.403413521011352	31.65199108825832	28.44481214994974	21.499783240780587
40	20.12665725524073	30.525510098756452	27.989252553868237	21.358580092134577
41	17.454659211631633	30.264096892165064	29.294444770862476	22.98679912534082
42	19.739126706482608	30.68484797477018	28.12502481218171	21.451000506565503
43	19.431312735120233	30.51982513168321	29.505137908090916	20.54372422510564
44	19.359663094241828	32.04977998542235	27.953872367725268	20.63668455261056
45	18.54560121702759	32.6058586920447	27.1846867948378	21.66385329608992
46	20.727485227819496	31.409474839256763	26.8281853680381	21.03485456488564
47	19.01770756084741	30.562859379639878	28.76291622931061	21.6565168302021
48	18.84591992453921	29.849157815003547	29.951582501099676	21.353339759357567
49	20.1760116621224	28.952393958690298	29.34687985822518	21.524714520962124
50	18.19853238922651	30.85384076693064	29.870881376333703	21.07674546750914
51	17.257019266956846	30.31958090041621	29.14739785718029	23.276001975446658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2457.0
1	3211.0
2	3965.0
3	34246.0
4	64527.0
5	40632.5
6	16738.0
7	18827.5
8	20917.0
9	26483.5
10	32050.0
11	37860.5
12	43671.0
13	45567.0
14	47463.0
15	45234.0
16	43005.0
17	39595.5
18	36186.0
19	33038.5
20	29891.0
21	28721.0
22	27551.0
23	28836.5
24	30122.0
25	29715.0
26	31579.0
27	33850.0
28	40266.0
29	46682.0
30	52481.0
31	58280.0
32	67353.5
33	76427.0
34	86363.5
35	96300.0
36	104189.5
37	112079.0
38	119826.5
39	127574.0
40	147295.0
41	167016.0
42	183125.0
43	199234.0
44	215357.0
45	231480.0
46	257951.0
47	284422.0
48	296112.0
49	307802.0
50	284890.5
51	261979.0
52	226534.5
53	191090.0
54	168432.0
55	145774.0
56	125336.5
57	104899.0
58	94866.5
59	84834.0
60	74835.0
61	64836.0
62	56849.5
63	48863.0
64	39876.0
65	30889.0
66	24506.0
67	18123.0
68	14434.5
69	10746.0
70	8812.5
71	6879.0
72	5504.5
73	4130.0
74	3629.5
75	2611.0
76	2093.0
77	1421.0
78	749.0
79	544.5
80	340.0
81	276.0
82	212.0
83	127.5
84	43.0
85	31.5
86	20.0
87	14.5
88	9.0
89	11.0
90	13.0
91	9.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3148655.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.102048636327762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66804921346638	16.50316855295749
2	9.659102480999028	4.269719056366294
3	3.858875169141937	2.5586714000967783
4	2.153280385889727	1.9036763126634142
5	1.4390299221514173	1.590275466426079
6	1.0361492372369652	1.374061250154319
7	0.7353433644234322	1.1376816363421312
8	0.5496121134070492	0.9718042929309993
9	0.4363201871233498	0.8679212997130719
>10	3.5018693260895386	17.163253632281616
>50	1.03984432609536	16.58324293891545
>100	0.9164027106642759	31.357603172844676
>500	0.002915030148367563	0.46285174422233827
>1k	0.003060781655785941	1.5708546494650528
>5k	0.0	0.0
>10k+	1.4575150741837816E-4	1.6852145946202102
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52313	1.6614395670532338	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	4409	0.14002804372025515	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT	4223	0.13412075949889715	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	3502	0.11122209324298789	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	3385	0.10750622091019817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.351918517589256E-5	0.0	0.0	0.15047694968168948	0.0
2	6.351918517589256E-5	0.0	0.0	0.42144979364204715	0.0
3	9.527877776383885E-5	0.0	0.0	0.6894689954917258	0.0
4	9.527877776383885E-5	0.0	0.0	1.0986595863948256	0.0
5	9.527877776383885E-5	0.0	0.0	1.8330366458059075	0.0
6	9.527877776383885E-5	0.0	0.0	3.1738631256838237	0.0
7	9.527877776383885E-5	0.0	0.0	3.976459789973814	0.0
8	9.527877776383885E-5	0.0	0.0	5.614746614030436	0.0
9	9.527877776383885E-5	0.0	0.0	6.400637732619166	0.0
10	9.527877776383885E-5	0.0	0.0	7.341388624666723	0.0
11	9.527877776383885E-5	0.0	0.0	8.280932652195938	0.0
12	9.527877776383885E-5	0.0	0.0	8.99149001716606	0.0
13	9.527877776383885E-5	0.0	0.0	9.36990556285144	0.0
14	9.527877776383885E-5	0.0	0.0	9.561924059638162	0.0
15	9.527877776383885E-5	0.0	0.0	9.757817226720615	0.0
16	9.527877776383885E-5	0.0	0.0	10.080081812710507	0.0
17	9.527877776383885E-5	0.0	0.0	10.465420949580059	0.0
18	1.2703837035178513E-4	0.0	0.0	10.885187484814944	0.0
19	1.2703837035178513E-4	0.0	0.0	11.142535463555074	0.0
20	1.2703837035178513E-4	0.0	0.0	11.401566700702363	0.0
21	1.2703837035178513E-4	0.0	0.0	11.722465624210972	0.0
22	1.2703837035178513E-4	0.0	0.0	12.071312989196974	0.0
23	1.2703837035178513E-4	0.0	0.0	12.428386088663254	0.0
24	1.2703837035178513E-4	0.0	0.0	12.702090257586176	0.0
25	1.2703837035178513E-4	0.0	0.0	12.944955862106202	0.0
26	1.2703837035178513E-4	0.0	0.0	13.16565327099984	0.0
27	1.2703837035178513E-4	0.0	0.0	13.381110347116467	0.0
28	1.2703837035178513E-4	0.0	0.0	13.616988841267144	0.0
29	1.2703837035178513E-4	0.0	0.0	13.858075908602244	0.0
30	1.2703837035178513E-4	0.0	0.0	14.163380872150173	0.0
31	1.2703837035178513E-4	0.0	0.0	14.41904559248314	0.0
32	1.2703837035178513E-4	0.0	0.0	14.66769144285417	0.0
33	1.2703837035178513E-4	0.0	0.0	14.922276337039149	0.0
34	1.2703837035178513E-4	0.0	0.0	15.165173701151762	0.0
35	1.2703837035178513E-4	0.0	0.0	15.42236288192895	0.0
36	1.2703837035178513E-4	0.0	0.0	15.669770108189052	0.0
37	1.2703837035178513E-4	0.0	0.0	15.915494076041993	0.0
38	1.2703837035178513E-4	0.0	0.0	16.176970801818555	0.0
39	1.2703837035178513E-4	0.0	0.0	16.441210612150268	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	55	1.8189894E-12	44.999996	1
CGTTTTT	18105	0.0	43.334713	1
GCGATAT	245	0.0	42.2449	9
ATACCGG	50	1.0822987E-9	40.5	2
TAGGGCG	1210	0.0	40.35124	5
AGGGCGA	3050	0.0	40.204918	6
TAATGCG	140	0.0	40.17857	1
ATTCGGC	45	1.9301297E-8	40.0	17
TATACGA	45	1.9301297E-8	40.0	26
CGACGGT	575	0.0	39.913044	28
CGGTCTA	575	0.0	39.913044	31
CGTAAGG	645	0.0	39.76744	2
TATAGCG	125	0.0	39.6	1
GGGCGAT	5715	0.0	39.52756	7
CGGCATA	40	3.460773E-7	39.375004	25
ATTACGT	40	3.460773E-7	39.375004	12
TCGGAAC	40	3.460773E-7	39.375004	40
CGTACCG	40	3.460773E-7	39.375004	45
ACGTACC	40	3.460773E-7	39.375004	16
CGAGGGA	2085	0.0	39.06475	4
>>END_MODULE