FastQCFastQC Report
Thu 26 May 2016
SRR1547474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547474_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3430777
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174280.5079898810094623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACG1300.041.538461
CGTTTTT98150.041.355071
TACGGGA15750.040.04
GGCGATA10500.039.428578
TAAGGGA66900.039.417044
CGACGGT8500.039.1764728
CACGACG8450.039.1420126
TAGGGAC49150.038.7283825
GTATACG1050.038.571431
TCACGAC8650.038.2369925
TTAGGGA61350.038.215164
GGGCGAT54800.038.1843077
TATGGGA61000.038.028694
GACCGAT18600.037.8629049
TAGGGAG61700.037.5972445
ATAGGGA69200.037.4241334
AAGGGAC55600.037.1088145
CGTAAGG5100.037.0588232
AAGGGAT67300.036.5416035
ATGGGAC38150.036.507215
Produced by FastQC (version 0.11.5)