##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547474_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3430777
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26110674054303	31.0	31.0	33.0	30.0	34.0
2	31.67083753913472	31.0	31.0	34.0	30.0	34.0
3	31.836288397642868	31.0	31.0	34.0	30.0	34.0
4	35.59010801343253	37.0	35.0	37.0	33.0	37.0
5	35.46029310561427	37.0	35.0	37.0	33.0	37.0
6	35.508925820593994	37.0	35.0	37.0	33.0	37.0
7	35.905547926898194	37.0	35.0	37.0	35.0	37.0
8	35.940152915797206	37.0	35.0	37.0	35.0	37.0
9	37.69311091918828	39.0	37.0	39.0	35.0	39.0
10	36.97203548933667	39.0	37.0	39.0	32.0	39.0
11	36.79768926980681	39.0	37.0	39.0	32.0	39.0
12	36.432656800485724	39.0	35.0	39.0	32.0	39.0
13	36.31535217823834	39.0	35.0	39.0	32.0	39.0
14	37.37856176603726	39.0	36.0	41.0	32.0	41.0
15	37.48965671624824	39.0	36.0	41.0	32.0	41.0
16	37.52185204692698	39.0	36.0	41.0	32.0	41.0
17	37.453676237190585	39.0	36.0	41.0	32.0	41.0
18	37.35449753802127	39.0	36.0	41.0	32.0	41.0
19	37.30725751047066	39.0	36.0	41.0	32.0	41.0
20	37.20174846689248	39.0	35.0	41.0	32.0	41.0
21	37.15246400450977	39.0	35.0	41.0	31.0	41.0
22	37.08614462554692	39.0	35.0	41.0	31.0	41.0
23	37.001100916789404	39.0	35.0	41.0	31.0	41.0
24	36.92324712448521	39.0	35.0	41.0	31.0	41.0
25	36.80558893801608	39.0	35.0	41.0	31.0	41.0
26	36.68459827030436	38.0	35.0	40.0	31.0	41.0
27	36.59728714515691	38.0	35.0	40.0	30.0	41.0
28	36.50843963335419	38.0	35.0	40.0	30.0	41.0
29	36.55502733054349	38.0	35.0	40.0	30.0	41.0
30	36.49518578444475	38.0	35.0	40.0	30.0	41.0
31	36.40036877943393	38.0	35.0	40.0	30.0	41.0
32	36.38372677676223	39.0	35.0	41.0	30.0	41.0
33	36.304304826574274	39.0	35.0	41.0	30.0	41.0
34	36.18048068994283	39.0	35.0	41.0	30.0	41.0
35	36.08900228723697	39.0	35.0	41.0	29.0	41.0
36	36.03552897783796	39.0	35.0	41.0	29.0	41.0
37	35.989583117760205	38.0	35.0	41.0	29.0	41.0
38	35.87571678369069	38.0	35.0	41.0	28.0	41.0
39	35.79955940010091	38.0	35.0	41.0	28.0	41.0
40	35.69108455606412	38.0	35.0	40.0	27.0	41.0
41	35.59271587748198	38.0	34.0	40.0	27.0	41.0
42	35.56324850026685	38.0	34.0	40.0	27.0	41.0
43	35.54615324750049	38.0	34.0	40.0	27.0	41.0
44	35.43211290037213	38.0	34.0	40.0	27.0	41.0
45	35.306561166756104	38.0	34.0	40.0	26.0	41.0
46	35.2690655790219	38.0	34.0	40.0	26.0	41.0
47	35.225339041272576	38.0	34.0	40.0	26.0	41.0
48	35.14706143826894	38.0	34.0	40.0	26.0	41.0
49	35.055267946590526	38.0	34.0	40.0	26.0	41.0
50	34.96333017272764	37.0	34.0	40.0	26.0	41.0
51	34.397269481519785	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	3.0
13	16.0
14	24.0
15	110.0
16	250.0
17	577.0
18	1356.0
19	2431.0
20	4270.0
21	7069.0
22	10830.0
23	16085.0
24	23517.0
25	33212.0
26	44463.0
27	53801.0
28	61710.0
29	71799.0
30	86045.0
31	104028.0
32	127986.0
33	160041.0
34	232276.0
35	297561.0
36	274940.0
37	356371.0
38	533196.0
39	926407.0
40	399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.19475442443505	24.63197112490844	29.351630840477245	12.821643610179267
2	29.906665457999747	25.740728703730966	30.666231002481364	13.686374835787928
3	28.766661313166086	25.703186187851905	31.819468301204072	13.710684197777937
4	25.234108774776093	28.59212359182774	31.369307885648062	14.804459747748105
5	22.765688355728166	32.89939859104803	29.96335815472705	14.371554898496756
6	22.089806478240934	41.51517280196293	26.24259752236884	10.152423197427288
7	91.23017905273355	2.5375301280147324	5.093889809801103	1.1384010094506287
8	91.88032332034405	2.0121972369524457	4.8058792512599915	1.3016001914435127
9	86.27141898176419	5.6141509634697915	6.12144129449393	1.9929887602720902
10	29.19633074373531	45.47200240645195	12.98437642551527	12.34729042429747
11	31.72843935936378	24.40330572345565	26.291536873425464	17.5767180437551
12	31.73770839666933	22.412677944384026	26.42101191654252	19.428601742404126
13	26.387200333918525	25.36990891567712	28.196615518875166	20.046275231529183
14	21.350731918746103	28.259866496714885	29.12028383074738	21.269117753791633
15	20.48588410147322	26.515159685400715	33.15715361272388	19.841802600402183
16	24.749903593267646	25.453942357664168	31.037400565527868	18.758753483540318
17	24.638558553936907	25.35664661387202	27.434106034871984	22.57068879731909
18	24.53791079979841	25.52378076453235	30.123584249282302	19.814724186386933
19	25.396287779707045	26.66958534466099	27.160436251030013	20.773690624601947
20	26.602049623161168	26.66107415317288	27.627415014149854	19.109461209516095
21	25.94321927656621	26.21589803126231	29.010629370547836	18.830253321623644
22	25.52351843328785	23.64773344347359	29.121799522382247	21.706948600856308
23	22.800665854994364	26.549321043017372	29.299805845731157	21.35020725625711
24	22.333832831454796	26.21927918952471	31.251521156869128	20.195366822151367
25	22.522536439995953	28.160384659218597	29.07166510676736	20.24541379401809
26	22.325671414959352	28.648262478149995	27.389742906636016	21.636323200254637
27	20.886172432658842	28.305978499914158	29.246377715602033	21.561471351824967
28	19.262808395882335	28.193875614765986	30.827827049091212	21.71548894026047
29	21.56934128915986	26.19182185260074	30.213447274480387	22.025389583759015
30	22.28847867407296	27.460105975993194	29.917537630688322	20.333877719245525
31	23.659159426567218	27.390180125376844	28.443410924114275	20.507249523941663
32	24.663101099255357	26.63918406821545	28.410998441460926	20.286716391068264
33	24.037499376963297	27.045535165940542	27.747154653304484	21.169810803791677
34	21.577998220228245	27.394727200281455	29.276254329558583	21.75102024993172
35	21.67351594114103	27.85348625107374	28.628354451484313	21.84464335630092
36	23.941661028973904	28.466554369462077	27.50324489175484	20.08853970980918
37	22.097414084331334	29.745623221794947	27.790876527387237	20.366086166486483
38	22.320774565062084	28.727428218155833	28.25724318426992	20.69455403251217
39	21.835316023163266	28.097658343867877	27.839640990947533	22.227384642021324
40	23.223718708619067	27.034808732832243	28.415195741372873	21.326276817175817
41	20.945634181411382	26.863564726008132	28.824170151542933	23.366630941037556
42	22.071093516133516	26.596598962858852	28.10535339370644	23.22695412730119
43	21.65637696650059	27.043174184740078	28.55906985502118	22.741378993738152
44	21.721610002632058	27.280525665177308	28.2675906944695	22.730273637721137
45	21.303687182233062	28.786015529426717	26.767172567613688	23.14312472072653
46	21.53611266485697	28.466088002805197	28.20728365615136	21.790515676186473
47	21.316395673633114	27.9020758271377	28.976584604595402	21.804943894633784
48	21.379005397319617	27.815156741461188	29.447061117641866	21.35877674357733
49	21.750495587442728	27.042241451426307	29.438870553230363	21.7683924079006
50	21.296662534463767	28.016364805990012	29.007947762270764	21.679024897275458
51	20.92193692565853	28.43944680753077	27.971447867349	22.667168399461694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1421.0
1	3019.5
2	4618.0
3	16687.5
4	28757.0
5	20126.0
6	11495.0
7	11371.5
8	11248.0
9	11954.0
10	12660.0
11	12998.0
12	13336.0
13	13261.0
14	13186.0
15	12978.5
16	12771.0
17	12108.5
18	11446.0
19	11511.5
20	11577.0
21	12083.5
22	12590.0
23	14675.5
24	16761.0
25	19276.5
26	27989.5
27	34187.0
28	43356.5
29	52526.0
30	58820.0
31	65114.0
32	75242.5
33	85371.0
34	100036.5
35	114702.0
36	128269.5
37	141837.0
38	147840.0
39	153843.0
40	171390.5
41	188938.0
42	205212.5
43	221487.0
44	232955.0
45	244423.0
46	259748.5
47	275074.0
48	275770.5
49	276467.0
50	271940.0
51	267413.0
52	251243.0
53	235073.0
54	219572.5
55	204072.0
56	192081.5
57	180091.0
58	163049.0
59	146007.0
60	131641.0
61	117275.0
62	102865.5
63	88456.0
64	73777.5
65	59099.0
66	48155.0
67	37211.0
68	30808.5
69	24406.0
70	19129.0
71	13852.0
72	10959.0
73	8066.0
74	6846.5
75	4482.5
76	3338.0
77	2389.0
78	1440.0
79	1137.5
80	835.0
81	658.0
82	481.0
83	371.5
84	262.0
85	160.5
86	59.0
87	62.0
88	65.0
89	37.5
90	10.0
91	7.5
92	5.0
93	3.0
94	1.0
95	3.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3430777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.203036629668873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21539832560744	12.673269640739587
2	7.3935959318780515	2.3959741141838165
3	2.439809105417825	1.18596948913454
4	1.2513481246647937	0.8110255800164442
5	0.8244263275876011	0.6679104992182646
6	0.5398663523320557	0.5248484569177212
7	0.44468282193371733	0.504364843666358
8	0.36065608358139595	0.46749789863858215
9	0.29159621508649686	0.42522697387073805
>10	4.6104012144198165	19.02494065277642
>50	2.145837176755206	25.033280452454438
>100	1.4756268546435374	34.94904830267123
>500	0.006207725598211753	0.7145901902731421
>1k	3.6516032930657366E-4	0.10647112791525723
>5k	0.0	0.0
>10k+	1.8258016465328683E-4	0.5155817775235073
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17428	0.5079898810094623	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.829583211033536E-5	0.0	0.0	0.03101338268269841	0.0
2	5.829583211033536E-5	0.0	0.0	0.0844998086439311	0.0
3	5.829583211033536E-5	0.0	0.0	0.12807594314640677	0.0
4	5.829583211033536E-5	0.0	0.0	0.22397258696790845	0.0
5	5.829583211033536E-5	0.0	0.0	0.37898120454929013	0.0
6	5.829583211033536E-5	0.0	0.0	0.6087833747282321	0.0
7	5.829583211033536E-5	0.0	0.0	0.7164849245520767	0.0
8	5.829583211033536E-5	0.0	0.0	0.9914663646165286	0.0
9	5.829583211033536E-5	0.0	0.0	1.0963988624151322	0.0
10	5.829583211033536E-5	0.0	0.0	1.3041943559724225	0.0
11	5.829583211033536E-5	0.0	0.0	1.5410211739206598	0.0
12	5.829583211033536E-5	0.0	0.0	1.7447068113141717	0.0
13	5.829583211033536E-5	0.0	0.0	1.832208855311785	0.0
14	5.829583211033536E-5	0.0	0.0	1.878087675182619	0.0
15	5.829583211033536E-5	0.0	0.0	1.9373162406067197	0.0
16	5.829583211033536E-5	0.0	0.0	2.0415491884199994	0.0
17	5.829583211033536E-5	0.0	0.0	2.1721027044310954	0.0
18	5.829583211033536E-5	0.0	0.0	2.320990259640892	0.0
19	5.829583211033536E-5	0.0	0.0	2.4089003744632778	0.0
20	5.829583211033536E-5	0.0	0.0	2.5116176306416884	0.0
21	5.829583211033536E-5	0.0	0.0	2.6416173362477364	0.0
22	5.829583211033536E-5	0.0	0.0	2.8002985912520693	0.0
23	5.829583211033536E-5	0.0	0.0	2.9497982526990243	0.0
24	5.829583211033536E-5	0.0	0.0	3.0685760106238322	0.0
25	5.829583211033536E-5	0.0	0.0	3.1823111790710965	0.0
26	5.829583211033536E-5	0.0	0.0	3.2914409767816446	0.0
27	5.829583211033536E-5	0.0	0.0	3.4138330762972937	0.0
28	5.829583211033536E-5	0.0	0.0	3.5377991632799217	0.0
29	8.744374816550303E-5	0.0	0.0	3.682343678997498	0.0
30	8.744374816550303E-5	0.0	0.0	3.842074258979817	0.0
31	8.744374816550303E-5	0.0	0.0	3.985627745551518	0.0
32	8.744374816550303E-5	0.0	0.0	4.115977226150227	0.0
33	8.744374816550303E-5	0.0	0.0	4.259384973141652	0.0
34	8.744374816550303E-5	0.0	0.0	4.412411532431283	0.0
35	8.744374816550303E-5	0.0	0.0	4.576601743570043	0.0
36	8.744374816550303E-5	0.0	0.0	4.729628302859673	0.0
37	8.744374816550303E-5	0.0	0.0	4.894022549410819	0.0
38	8.744374816550303E-5	0.0	0.0	5.083338264189132	0.0
39	8.744374816550303E-5	0.0	0.0	5.401487767931288	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGACG	130	0.0	41.53846	1
CGTTTTT	9815	0.0	41.35507	1
TACGGGA	1575	0.0	40.0	4
GGCGATA	1050	0.0	39.42857	8
TAAGGGA	6690	0.0	39.41704	4
CGACGGT	850	0.0	39.17647	28
CACGACG	845	0.0	39.14201	26
TAGGGAC	4915	0.0	38.728382	5
GTATACG	105	0.0	38.57143	1
TCACGAC	865	0.0	38.23699	25
TTAGGGA	6135	0.0	38.21516	4
GGGCGAT	5480	0.0	38.184307	7
TATGGGA	6100	0.0	38.02869	4
GACCGAT	1860	0.0	37.862904	9
TAGGGAG	6170	0.0	37.597244	5
ATAGGGA	6920	0.0	37.424133	4
AAGGGAC	5560	0.0	37.108814	5
CGTAAGG	510	0.0	37.058823	2
AAGGGAT	6730	0.0	36.541603	5
ATGGGAC	3815	0.0	36.50721	5
>>END_MODULE