FastQCFastQC Report
Thu 26 May 2016
SRR1547473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547473_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1826486
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204711.1207860339471531No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48190.2638399637336393No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT34550.18916104476026643TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC32020.17530930978939888TruSeq Adapter, Index 13 (95% over 23bp)
GAGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT26220.1435543442435365TruSeq Adapter, Index 13 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT24210.13254960618367728No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC23470.12849811057955002TruSeq Adapter, Index 13 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21200.11606987406418663No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT19480.10665288428162055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA253.8917125E-545.028
CTATGCG900.045.01
AGCGTAA207.034381E-445.01
TCGCAAG207.034381E-445.01
TAGCGAG1000.045.01
CTAAGCG253.8917125E-545.01
CGCGACG253.8917125E-545.036
ATGTCGA253.8917125E-545.028
ACGATTT207.034381E-445.045
CACTCGA207.034381E-445.039
TATTACG207.034381E-445.01
CGTCGTG207.034381E-445.034
TCGGTTA453.8562575E-1045.01
AGTCGGT406.8193913E-945.032
CTATCCG302.1662072E-644.9999961
GCGTAAT302.1662072E-644.9999969
GACGGTC11400.044.60526329
TCACGAC11700.044.23076625
CGGTCTA11550.044.2207831
CGACGGT11500.044.21739228