FastQCFastQC Report
Thu 26 May 2016
SRR1547472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547472_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3543691
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278050.7846338746803827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG1950.042.6923071
CGTTTTT137600.041.795061
CGACGGT8250.040.3636428
CACGACG8300.039.849426
TCACGAC8450.039.40828325
TCGTTAG1200.039.3749961
TAAGGGA76650.038.8356174
TACGGGA21800.038.6009144
CGTAAGG5050.038.3168332
CGGTCTA8700.038.2758631
AAGGGAT84800.038.0218165
AGGGATC65650.037.7341966
TAGGGAC54300.037.5828745
ATTAGCG2700.037.51
CGAACGC301.1404021E-437.49999624
ACGTTAC301.1404021E-437.49999636
TATGGGA60450.037.2208444
ATAGGGA83500.036.916174
TAGGGCG10750.036.8372085
AGGGATG102450.036.80826
Produced by FastQC (version 0.11.5)