##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547472_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3543691
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35557304516675	31.0	31.0	33.0	30.0	34.0
2	31.792709635236257	31.0	31.0	34.0	30.0	34.0
3	31.923726984096525	31.0	31.0	34.0	30.0	34.0
4	35.67673084363168	37.0	35.0	37.0	33.0	37.0
5	35.565202214301415	37.0	35.0	37.0	33.0	37.0
6	35.60623570170198	37.0	35.0	37.0	33.0	37.0
7	35.98148145535262	37.0	35.0	37.0	35.0	37.0
8	36.01797504353512	37.0	35.0	37.0	35.0	37.0
9	37.75727990956322	39.0	38.0	39.0	35.0	39.0
10	37.12117168229397	39.0	37.0	39.0	33.0	39.0
11	36.978606486852264	39.0	37.0	39.0	33.0	39.0
12	36.64347484021604	39.0	35.0	39.0	32.0	39.0
13	36.55853571883102	39.0	35.0	39.0	32.0	39.0
14	37.652155901854876	40.0	36.0	41.0	32.0	41.0
15	37.75737670129816	40.0	36.0	41.0	32.0	41.0
16	37.7800999014869	40.0	36.0	41.0	33.0	41.0
17	37.713268171519466	40.0	36.0	41.0	32.0	41.0
18	37.59287195187165	39.0	36.0	41.0	32.0	41.0
19	37.53780450947896	39.0	36.0	41.0	32.0	41.0
20	37.417936552594455	39.0	35.0	41.0	32.0	41.0
21	37.37846640691866	39.0	35.0	41.0	32.0	41.0
22	37.30799976634532	39.0	35.0	41.0	32.0	41.0
23	37.22499027144297	39.0	35.0	41.0	32.0	41.0
24	37.15501012926917	39.0	35.0	41.0	32.0	41.0
25	37.05451745087255	39.0	35.0	41.0	31.0	41.0
26	36.93366295198989	39.0	35.0	41.0	31.0	41.0
27	36.826998177888534	39.0	35.0	41.0	31.0	41.0
28	36.71284262651569	38.0	35.0	40.0	31.0	41.0
29	36.76792417849073	39.0	35.0	40.0	31.0	41.0
30	36.712018909097885	39.0	35.0	41.0	31.0	41.0
31	36.62809991051703	39.0	35.0	41.0	30.0	41.0
32	36.57866303805834	39.0	35.0	41.0	30.0	41.0
33	36.50483097990203	39.0	35.0	41.0	30.0	41.0
34	36.35029549698323	39.0	35.0	41.0	30.0	41.0
35	36.23906824833204	39.0	35.0	41.0	30.0	41.0
36	36.19432309419755	39.0	35.0	41.0	29.0	41.0
37	36.16297103782468	39.0	35.0	41.0	29.0	41.0
38	36.05543654906706	39.0	35.0	41.0	29.0	41.0
39	35.972877432033435	39.0	35.0	41.0	29.0	41.0
40	35.88738521502016	39.0	35.0	41.0	28.0	41.0
41	35.788957050713506	38.0	35.0	41.0	27.0	41.0
42	35.74322337923933	38.0	35.0	41.0	27.0	41.0
43	35.728160553501986	38.0	35.0	41.0	27.0	41.0
44	35.602759664993364	38.0	35.0	40.0	27.0	41.0
45	35.48715139102139	38.0	34.0	40.0	27.0	41.0
46	35.442968080456225	38.0	34.0	40.0	27.0	41.0
47	35.388429747401794	38.0	34.0	40.0	27.0	41.0
48	35.3203134810569	38.0	34.0	40.0	26.0	41.0
49	35.24149114581378	38.0	34.0	40.0	26.0	41.0
50	35.163101410365634	38.0	34.0	40.0	26.0	41.0
51	34.60399792194071	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	5.0
14	13.0
15	55.0
16	144.0
17	445.0
18	1051.0
19	2057.0
20	3759.0
21	5937.0
22	9433.0
23	14363.0
24	22064.0
25	32917.0
26	44243.0
27	53030.0
28	59686.0
29	68005.0
30	81398.0
31	99191.0
32	123955.0
33	156550.0
34	227728.0
35	293087.0
36	290354.0
37	375413.0
38	568183.0
39	1010120.0
40	502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.395285593467374	24.606123953809742	30.02451398838104	14.97407646434184
2	30.45564638677582	25.929574559407126	29.994629892956244	13.620149160860809
3	27.45301438528359	26.22435195393729	32.47946844123825	13.843165219540868
4	24.923730652587935	28.572496868378195	31.59471297017714	14.909059508856725
5	22.756019077284108	32.93822740188126	30.04339260957008	14.262360911264555
6	22.20845440530791	40.77550779681411	26.449174039158606	10.56686375871937
7	90.34176512568392	2.5055231960122932	5.925290890204591	1.227420788099188
8	90.74834685078355	2.147280900055902	5.719911809466457	1.384460439694093
9	83.93110460251755	6.527911152524303	7.325187212993457	2.2157970319646942
10	26.54336961095084	50.59560779989	12.551432955074244	10.309589634084913
11	31.524531907550628	22.92025461587932	28.500227587563366	17.054985889006687
12	30.622647403512325	21.285800596045195	28.394716130723584	19.69683586971889
13	26.136138845062955	24.188931822780262	30.012125775074633	19.66280355708215
14	21.271352383715172	27.102080852986337	30.542251003261857	21.084315760036638
15	19.96237820961252	26.270208096586302	34.12298081294335	19.64443288085784
16	24.264051239230508	25.855019526251017	31.513836844126647	18.367092390391825
17	24.157636769120106	25.154732734880103	29.178446992133345	21.50918350386645
18	23.08505453776867	25.359349898171146	31.38978539607432	20.165810167985864
19	24.503434413440676	26.34213874742465	28.76610855743348	20.3883182817012
20	25.13627740116167	26.644873946402214	29.187420686510197	19.031427965925925
21	25.408169053114392	25.058815794040733	31.237655879138448	18.295359273706428
22	24.80394029840638	23.324465931143546	30.550152369379834	21.32144140107024
23	21.88407510700002	26.11610323812093	30.73137584512871	21.268445809750343
24	21.091285893719288	26.267922344245026	31.78457151032638	20.856220251709306
25	21.81677239917363	27.77522080790904	29.553197499443378	20.854809293473952
26	21.726132442134485	28.48081280224489	28.31807287937916	21.47498187624147
27	20.47994590950509	27.905282938043978	30.6476495834428	20.967121569008132
28	19.31759851522043	27.42894343778845	32.266893473499806	20.98656457349131
29	21.170412431557946	25.119486998160955	31.748366322007193	21.961734248273903
30	21.717074090263512	26.203667306207002	31.196032611195502	20.88322599233398
31	22.68507609721051	26.35119709929562	29.96573346829619	20.99799333519768
32	23.12247315017026	26.208492783371913	30.11938682012625	20.549647246331578
33	23.355591669815457	26.097393931920138	29.50214903048827	21.04486536777614
34	21.1359568314506	26.684804064462732	30.5400499084147	21.63918919567197
35	21.434769566533877	26.71209199673448	30.297844817733825	21.55529361899782
36	23.22967775689246	26.45786554188839	29.249361753042237	21.063094948176914
37	21.654907270413815	28.345784099121506	29.361674028576417	20.63763460188826
38	21.411037249015223	27.998885907377364	28.981420784148504	21.60865605945891
39	21.137113817203588	27.7180769993772	29.069069509728696	22.07573967369051
40	22.21968563286133	27.093869076056578	29.075051972646598	21.611393318435496
41	20.884439416416388	26.186368958241562	29.699739621767247	23.229452003574806
42	21.076132202271587	26.78131360776095	29.451298095686106	22.69125609428136
43	21.226540350160327	26.72521390832327	29.94575429968358	22.10249144183282
44	21.645199877754578	27.36528100220928	29.171194666803622	21.818324453232517
45	21.01294949249243	27.73548822400147	28.53098647709408	22.720575806412015
46	21.676579588908854	27.703600567882468	29.007495292337847	21.612324550870827
47	20.981202932196965	27.026989655700795	29.978742503226158	22.013064908876085
48	21.14501518332157	27.122652624057796	30.23770977774304	21.494622414877597
49	21.335127695953172	26.66733640150905	29.93556718122432	22.061968721313455
50	20.665486917454146	26.99995569591141	30.308314127840152	22.02624325879429
51	19.974597107930688	27.554321186582015	29.57345885970306	22.89762284578424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1895.0
1	3291.0
2	4687.0
3	23607.5
4	42528.0
5	28581.0
6	14634.0
7	14690.0
8	14746.0
9	15529.0
10	16312.0
11	16427.5
12	16543.0
13	16363.5
14	16184.0
15	15553.0
16	14922.0
17	14395.5
18	13869.0
19	13939.0
20	14009.0
21	14258.5
22	14508.0
23	16990.5
24	19473.0
25	25101.5
26	36813.0
27	42896.0
28	48980.0
29	55064.0
30	64617.0
31	74170.0
32	87157.0
33	100144.0
34	113303.5
35	126463.0
36	134307.0
37	142151.0
38	153113.0
39	164075.0
40	179330.0
41	194585.0
42	214745.0
43	234905.0
44	243924.0
45	252943.0
46	261139.5
47	269336.0
48	276055.0
49	282774.0
50	273043.0
51	263312.0
52	242964.0
53	222616.0
54	210662.5
55	198709.0
56	186023.0
57	173337.0
58	158151.5
59	142966.0
60	127339.0
61	111712.0
62	99173.5
63	86635.0
64	74562.0
65	62489.0
66	52011.0
67	41533.0
68	34216.0
69	26899.0
70	21913.0
71	16927.0
72	13644.0
73	10361.0
74	7979.0
75	4406.5
76	3216.0
77	2408.0
78	1600.0
79	1132.0
80	664.0
81	520.0
82	376.0
83	246.5
84	117.0
85	85.0
86	53.0
87	35.5
88	18.0
89	11.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3543691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.618235110425193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.83843133447655	13.5375754876707
2	7.76231011492056	2.73516409209404
3	2.6880665161437998	1.4207696362164903
4	1.4569992441426105	1.0267902095606523
5	0.9161791041822748	0.8070729430371025
6	0.6680359802240134	0.7061768977086014
7	0.5387953197058368	0.6644835833281898
8	0.4032146495533852	0.5683144396639392
9	0.36109158980060746	0.5725616872953883
>10	4.916972625788258	21.90456525464022
>50	2.383435621487714	30.341067352070755
>100	1.0622666037427204	24.282328613351723
>500	0.0033933543257369256	0.4247860507740912
>1k	6.463532049022716E-4	0.216763017934048
>5k	0.0	0.0
>10k+	1.615883012255679E-4	0.7915807346540852
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27805	0.7846338746803827	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6438329414161676E-5	0.0	0.0	0.029799437930677363	0.0
2	5.6438329414161676E-5	0.0	0.0	0.09653776246292355	0.0
3	5.6438329414161676E-5	0.0	0.0	0.15495143340658088	0.0
4	5.6438329414161676E-5	0.0	0.0	0.25617357721087985	0.0
5	5.6438329414161676E-5	0.0	0.0	0.4327973291124988	0.0
6	5.6438329414161676E-5	0.0	0.0	0.7048582960534652	0.0
7	5.6438329414161676E-5	0.0	0.0	0.8498483643184465	0.0
8	5.6438329414161676E-5	0.0	0.0	1.2634566614301304	0.0
9	5.6438329414161676E-5	0.0	0.0	1.4423943848377299	0.0
10	5.6438329414161676E-5	0.0	0.0	1.7699906679222315	0.0
11	5.6438329414161676E-5	0.0	0.0	2.082235725406081	0.0
12	5.6438329414161676E-5	0.0	0.0	2.3702687395712547	0.0
13	5.6438329414161676E-5	0.0	0.0	2.5070470309064756	0.0
14	5.6438329414161676E-5	0.0	0.0	2.5789776817448247	0.0
15	5.6438329414161676E-5	0.0	0.0	2.6570318913246105	0.0
16	5.6438329414161676E-5	0.0	0.0	2.7952775792245994	0.0
17	5.6438329414161676E-5	0.0	0.0	2.962955855914074	0.0
18	5.6438329414161676E-5	0.0	0.0	3.150951931192646	0.0
19	5.6438329414161676E-5	0.0	0.0	3.2679768072329107	0.0
20	5.6438329414161676E-5	0.0	0.0	3.4124589305331643	0.0
21	5.6438329414161676E-5	0.0	0.0	3.5860067934817117	0.0
22	5.6438329414161676E-5	0.0	0.0	3.7801828658311347	0.0
23	5.6438329414161676E-5	0.0	0.0	3.974612910662922	0.0
24	5.6438329414161676E-5	0.0	0.0	4.124823524398713	0.0
25	5.6438329414161676E-5	0.0	0.0	4.2624483906751465	0.0
26	5.6438329414161676E-5	0.0	0.0	4.3975053129632355	0.0
27	5.6438329414161676E-5	0.0	0.0	4.546869351757814	0.0
28	5.6438329414161676E-5	0.0	0.0	4.693101063269907	0.0
29	5.6438329414161676E-5	0.0	0.0	4.856038520288592	0.0
30	5.6438329414161676E-5	0.0	0.0	5.062941435920909	0.0
31	5.6438329414161676E-5	0.0	0.0	5.232228204998687	0.0
32	5.6438329414161676E-5	0.0	0.0	5.396971688558624	0.0
33	5.6438329414161676E-5	0.0	0.0	5.567048594248201	0.0
34	5.6438329414161676E-5	0.0	0.0	5.750247411526569	0.0
35	5.6438329414161676E-5	0.0	0.0	5.949756906005631	0.0
36	5.6438329414161676E-5	0.0	0.0	6.137273255484183	0.0
37	5.6438329414161676E-5	0.0	0.0	6.331392889504192	0.0
38	5.6438329414161676E-5	0.0	0.0	6.541315255760167	0.0
39	5.6438329414161676E-5	0.0	0.0	6.874357837633134	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	195	0.0	42.692307	1
CGTTTTT	13760	0.0	41.79506	1
CGACGGT	825	0.0	40.36364	28
CACGACG	830	0.0	39.8494	26
TCACGAC	845	0.0	39.408283	25
TCGTTAG	120	0.0	39.374996	1
TAAGGGA	7665	0.0	38.835617	4
TACGGGA	2180	0.0	38.600914	4
CGTAAGG	505	0.0	38.316833	2
CGGTCTA	870	0.0	38.27586	31
AAGGGAT	8480	0.0	38.021816	5
AGGGATC	6565	0.0	37.734196	6
TAGGGAC	5430	0.0	37.582874	5
ATTAGCG	270	0.0	37.5	1
CGAACGC	30	1.1404021E-4	37.499996	24
ACGTTAC	30	1.1404021E-4	37.499996	36
TATGGGA	6045	0.0	37.220844	4
ATAGGGA	8350	0.0	36.91617	4
TAGGGCG	1075	0.0	36.837208	5
AGGGATG	10245	0.0	36.8082	6
>>END_MODULE