FastQCFastQC Report
Thu 26 May 2016
SRR1547471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547471_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5122889
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371300.7247863461417962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA6100.042.41803431
CGTTTTT193800.042.2020151
TAATGCG2150.040.813951
CGACGGT6700.039.9626928
CTCACGA6550.039.5038224
GGGCGAT87600.038.707197
CGTTAGG4200.038.571432
CGATCGA356.2525214E-638.57142610
TACGGGA29800.038.3557054
CGAGGGA45900.037.9411774
CACGACC11150.037.73542427
TTAGGGA95100.037.594644
AGGGCGA47050.037.5876736
TAAGGGA96800.037.4922524
ACGGGAC18550.037.4797825
ATAGGGA103500.037.456524
TAGGGAC71150.037.3471535
GACCGAT28350.036.9841279
TAGGGAT103450.036.822145
CGGGATC13700.036.788326
Produced by FastQC (version 0.11.5)