##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547471_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5122889
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260751892145233	31.0	31.0	33.0	30.0	34.0
2	31.669433985393788	31.0	31.0	34.0	30.0	34.0
3	31.800934199433172	31.0	31.0	34.0	30.0	34.0
4	35.56059696003564	37.0	35.0	37.0	33.0	37.0
5	35.43646036445451	37.0	35.0	37.0	33.0	37.0
6	35.48105453778132	37.0	35.0	37.0	33.0	37.0
7	35.91643328598375	37.0	35.0	37.0	35.0	37.0
8	35.954280680295824	37.0	35.0	37.0	35.0	37.0
9	37.67043381185889	39.0	37.0	39.0	35.0	39.0
10	36.95023179303709	39.0	37.0	39.0	32.0	39.0
11	36.790657576223104	39.0	37.0	39.0	32.0	39.0
12	36.44285831685988	39.0	35.0	39.0	32.0	39.0
13	36.34854180912372	39.0	35.0	39.0	32.0	39.0
14	37.40612787042624	39.0	36.0	41.0	32.0	41.0
15	37.51078444213802	39.0	36.0	41.0	32.0	41.0
16	37.54146869081099	40.0	36.0	41.0	32.0	41.0
17	37.46796055897366	39.0	36.0	41.0	32.0	41.0
18	37.36421714388112	39.0	36.0	41.0	32.0	41.0
19	37.30421935747583	39.0	36.0	41.0	32.0	41.0
20	37.1895104500605	39.0	35.0	41.0	32.0	41.0
21	37.12927803042385	39.0	35.0	41.0	31.0	41.0
22	37.06709866249298	39.0	35.0	41.0	31.0	41.0
23	36.95839691236722	39.0	35.0	41.0	31.0	41.0
24	36.89993575890479	39.0	35.0	41.0	31.0	41.0
25	36.79270934818225	39.0	35.0	41.0	31.0	41.0
26	36.64237230203504	38.0	35.0	40.0	31.0	41.0
27	36.54863730211605	38.0	35.0	40.0	30.0	41.0
28	36.44316439415338	38.0	35.0	40.0	30.0	41.0
29	36.47581784418909	38.0	35.0	40.0	30.0	41.0
30	36.41938699042669	38.0	35.0	40.0	30.0	41.0
31	36.31896377219963	38.0	35.0	40.0	30.0	41.0
32	36.25572933553704	38.0	35.0	41.0	30.0	41.0
33	36.159783083334425	38.0	35.0	41.0	30.0	41.0
34	36.01648737655647	38.0	35.0	41.0	29.0	41.0
35	35.89760914202904	38.0	35.0	41.0	28.0	41.0
36	35.86180005071357	38.0	35.0	41.0	28.0	41.0
37	35.81433815958144	38.0	35.0	41.0	28.0	41.0
38	35.7069040535526	38.0	35.0	41.0	27.0	41.0
39	35.626044210600696	38.0	35.0	40.0	27.0	41.0
40	35.54350777461702	38.0	34.0	40.0	26.0	41.0
41	35.43569302399486	38.0	34.0	40.0	26.0	41.0
42	35.39757488401564	38.0	34.0	40.0	26.0	41.0
43	35.385676714838056	38.0	34.0	40.0	26.0	41.0
44	35.26713910842105	38.0	34.0	40.0	26.0	41.0
45	35.13877540583058	38.0	34.0	40.0	25.0	41.0
46	35.12711811635973	38.0	34.0	40.0	26.0	41.0
47	35.08045909251596	38.0	34.0	40.0	26.0	41.0
48	34.98671843172866	38.0	34.0	40.0	25.0	41.0
49	34.902871407129844	38.0	34.0	40.0	25.0	41.0
50	34.80058459201439	37.0	34.0	40.0	24.0	41.0
51	34.26649611186188	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	2.0
12	7.0
13	13.0
14	26.0
15	103.0
16	346.0
17	950.0
18	2016.0
19	3885.0
20	6821.0
21	11040.0
22	16988.0
23	25177.0
24	37476.0
25	55014.0
26	72972.0
27	85969.0
28	96031.0
29	109249.0
30	129588.0
31	156940.0
32	190805.0
33	240176.0
34	342614.0
35	436205.0
36	417987.0
37	534032.0
38	789573.0
39	1360237.0
40	643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.89136266665157	24.69887596627606	28.827230103951113	13.58253126312126
2	31.066220642297736	25.57861394225016	29.434855996294278	13.920309419157823
3	28.724651266111756	25.75718505710352	31.84140823664147	13.676755440143248
4	25.121781869566178	28.077594497948326	31.589304394453986	15.211319238031509
5	22.84742066439464	32.547533237593086	30.618484999382183	13.98656109863009
6	21.93178497523565	40.77205264451367	27.161568404078245	10.134593976172429
7	89.73116536391868	2.645069998588687	6.373552110928032	1.2502125265646007
8	89.93226283060203	2.27719163932695	6.161191468329687	1.6293540617413338
9	83.33450129409403	6.274935880906263	7.925488918459876	2.465073906539845
10	28.94345358644312	45.202111542920406	14.492096159022772	11.3623387116137
11	31.686847011520257	24.22935574048159	26.961564070585954	17.1222331774122
12	29.225442909264675	22.62079072960589	28.750417977043814	19.40334838408562
13	25.32795459749372	25.608792226417552	30.468140145140758	18.595113030947967
14	21.012420921085738	28.50746912533143	30.88967963194205	19.590430321640778
15	19.86176159584953	26.800658768909496	34.4821642631726	18.855415372068375
16	24.300058033660303	26.217491731716226	31.66697541172569	17.815474822897784
17	23.272532354302424	25.838194034655054	29.104573610710677	21.784700000331846
18	23.76077248599374	24.938935823126364	31.241141473102385	20.059150217777507
19	24.369511031763523	26.929121439094228	28.916086216195584	19.78528131294666
20	25.979149655594725	26.745357941583354	28.832207764017532	18.443284638804393
21	24.991542857945976	26.120105276534396	30.64735542776742	18.240996437752212
22	24.952307184481256	23.485029638549655	30.181056040839454	21.38160713612963
23	21.242447376860987	27.437408852700106	30.58604236789046	20.734101402548443
24	20.780696204817243	26.681956216502055	32.421530117088224	20.11581746159247
25	21.85762760036378	27.83257650126715	30.05967921616104	20.250116682208027
26	21.295015371209487	29.09578950471111	28.69954824318856	20.909646880890843
27	20.648251406579373	28.422165695957887	30.212756903380107	20.716825994082637
28	19.650845450682223	27.782331414949653	31.271358797740884	21.295464336627244
29	20.659358420609934	26.610492634136712	31.175202117398992	21.55494682785436
30	21.895770140637442	26.96443354521248	30.118181362118136	21.02161495203195
31	22.918044876631136	27.42378372828301	29.032641542692023	20.625529852393836
32	23.79475721609428	26.556441101886065	29.079509628258588	20.56929205376107
33	23.255725431489928	27.385016540471597	28.611765743899586	20.74749228413889
34	20.922549756592424	27.439165673900018	30.41264411545907	21.225640454048484
35	20.656723188810062	28.099925647422776	29.941015704224704	21.30233545954246
36	23.2835027266841	27.92844037807573	27.789729584224837	20.998327311015327
37	21.115487764813956	29.4828172150519	28.561052171928768	20.84064284820538
38	21.794401557402473	28.94724051214071	28.38501868769751	20.873339242759307
39	21.147012945234614	28.12914744004799	28.69394593558439	22.029893679133007
40	23.51534456436593	26.604265678994803	28.85067390685217	21.029715849787102
41	20.546570499575534	26.802435110344963	28.998481130471497	23.652513259608007
42	21.61270720486038	26.809013429726857	28.950207587945005	22.628071777467753
43	21.179240854135234	26.894629182869274	29.34262288329886	22.583507079696634
44	21.43884046677568	27.896231989410662	29.12536266157631	21.539564882237347
45	20.53928945171367	28.321343679318446	28.056864007789358	23.08250286117853
46	21.248381528469583	28.362336173983078	28.19227588183152	22.19700641571582
47	21.258746773549067	27.69341674199851	29.253844851996597	21.79399163245583
48	21.008790157272585	28.00339808260534	29.848645949580405	21.13916581054167
49	22.10319216364048	26.70606370741197	29.684344126917452	21.506400002030105
50	20.40543919651587	28.58615519485197	29.148474620472943	21.85993098815922
51	20.327358254297526	28.432413819624042	28.488710178963473	22.751517747114956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2113.0
1	3909.0
2	5705.0
3	31507.0
4	57309.0
5	39267.5
6	21226.0
7	21075.0
8	20924.0
9	22098.5
10	23273.0
11	23826.5
12	24380.0
13	24557.5
14	24735.0
15	23822.0
16	22909.0
17	22374.5
18	21840.0
19	21502.0
20	21164.0
21	23365.0
22	25566.0
23	28316.0
24	31066.0
25	37849.0
26	50428.0
27	56224.0
28	69031.5
29	81839.0
30	92861.5
31	103884.0
32	120785.5
33	137687.0
34	154559.5
35	171432.0
36	186955.5
37	202479.0
38	218770.0
39	235061.0
40	262165.5
41	289270.0
42	312638.5
43	336007.0
44	352931.0
45	369855.0
46	387666.5
47	405478.0
48	415646.5
49	425815.0
50	415036.0
51	404257.0
52	379076.0
53	353895.0
54	325561.5
55	297228.0
56	273355.5
57	249483.0
58	225223.0
59	200963.0
60	175130.5
61	149298.0
62	126420.5
63	103543.0
64	89710.5
65	75878.0
66	61571.5
67	47265.0
68	38627.5
69	29990.0
70	24840.0
71	19690.0
72	16062.5
73	12435.0
74	10494.5
75	6483.5
76	4413.0
77	3444.0
78	2475.0
79	1781.0
80	1087.0
81	683.5
82	280.0
83	198.5
84	117.0
85	101.5
86	86.0
87	73.0
88	60.0
89	37.0
90	14.0
91	9.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5122889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.526400192311346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2439076576237	13.538076380254557
2	8.899156282646304	3.119403487671618
3	3.086839907308312	1.623035746352482
4	1.5512230674956062	1.0874942507389116
5	0.9963951805524889	0.873161034202662
6	0.6700004021919383	0.7045617106695281
7	0.4871846377488353	0.5977015050112624
8	0.3641214999299759	0.5105391301117941
9	0.301825115261073	0.4760916982340456
>10	3.3384233917869035	13.970030022750196
>50	1.5087738546807794	19.347671019327862
>100	1.5437734334937214	42.12313017586275
>500	0.006338268644673572	0.7389073008041228
>1k	0.0019241172671315148	0.5536498366514544
>5k	0.0	0.0
>10k+	1.1318336865479498E-4	0.7365467013568077
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37130	0.7247863461417962	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.856070666375945E-5	0.0	0.0	0.04161714220237839	0.0
2	5.856070666375945E-5	0.0	0.0	0.12049841407846237	0.0
3	5.856070666375945E-5	0.0	0.0	0.18872163734174213	0.0
4	5.856070666375945E-5	0.0	0.0	0.3167353421087203	0.0
5	5.856070666375945E-5	0.0	0.0	0.5485381393194348	0.0
6	5.856070666375945E-5	0.0	0.0	0.8960373726621834	0.0
7	5.856070666375945E-5	0.0	0.0	1.0777122049687198	0.0
8	5.856070666375945E-5	0.0	0.0	1.525116003879842	0.0
9	5.856070666375945E-5	0.0	0.0	1.7068298766574876	0.0
10	5.856070666375945E-5	0.0	0.0	2.049253848756044	0.0
11	7.808094221834593E-5	0.0	0.0	2.396245555974373	0.0
12	7.808094221834593E-5	0.0	0.0	2.699355773665992	0.0
13	7.808094221834593E-5	0.0	0.0	2.8537803571383256	0.0
14	7.808094221834593E-5	0.0	0.0	2.9284647783701736	0.0
15	7.808094221834593E-5	0.0	0.0	3.0200927640634023	0.0
16	7.808094221834593E-5	0.0	0.0	3.1896650503260955	0.0
17	7.808094221834593E-5	0.0	0.0	3.3978874029868695	0.0
18	9.760117777293242E-5	0.0	0.0	3.638415745490484	0.0
19	9.760117777293242E-5	0.0	0.0	3.7808158638611924	0.0
20	9.760117777293242E-5	0.0	0.0	3.9463669815996405	0.0
21	9.760117777293242E-5	0.0	0.0	4.155955750749236	0.0
22	9.760117777293242E-5	0.0	0.0	4.390862265413129	0.0
23	9.760117777293242E-5	0.0	0.0	4.654034081160064	0.0
24	9.760117777293242E-5	0.0	0.0	4.844141655226182	0.0
25	9.760117777293242E-5	0.0	0.0	5.022380926075111	0.0
26	9.760117777293242E-5	0.0	0.0	5.189161818653498	0.0
27	9.760117777293242E-5	0.0	0.0	5.374447894537633	0.0
28	9.760117777293242E-5	0.0	0.0	5.566761255221419	0.0
29	9.760117777293242E-5	0.0	0.0	5.7848218066017045	0.0
30	9.760117777293242E-5	0.0	0.0	6.050433651792963	0.0
31	1.171214133275189E-4	0.0	0.0	6.277746794826123	0.0
32	1.171214133275189E-4	0.0	0.0	6.492723148988784	0.0
33	1.171214133275189E-4	0.0	0.0	6.712735723924528	0.0
34	1.171214133275189E-4	0.0	0.0	6.948208325419505	0.0
35	1.171214133275189E-4	0.0	0.0	7.1974231727449105	0.0
36	1.171214133275189E-4	0.0	0.0	7.422432927982628	0.0
37	1.171214133275189E-4	0.0	0.0	7.664288646503955	0.0
38	1.171214133275189E-4	0.0	0.0	7.927148138482017	0.0
39	1.366416488821054E-4	0.0	0.0	8.33957167527932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	610	0.0	42.418034	31
CGTTTTT	19380	0.0	42.202015	1
TAATGCG	215	0.0	40.81395	1
CGACGGT	670	0.0	39.96269	28
CTCACGA	655	0.0	39.50382	24
GGGCGAT	8760	0.0	38.70719	7
CGTTAGG	420	0.0	38.57143	2
CGATCGA	35	6.2525214E-6	38.571426	10
TACGGGA	2980	0.0	38.355705	4
CGAGGGA	4590	0.0	37.941177	4
CACGACC	1115	0.0	37.735424	27
TTAGGGA	9510	0.0	37.59464	4
AGGGCGA	4705	0.0	37.587673	6
TAAGGGA	9680	0.0	37.492252	4
ACGGGAC	1855	0.0	37.479782	5
ATAGGGA	10350	0.0	37.45652	4
TAGGGAC	7115	0.0	37.347153	5
GACCGAT	2835	0.0	36.984127	9
TAGGGAT	10345	0.0	36.82214	5
CGGGATC	1370	0.0	36.78832	6
>>END_MODULE