SRR1547470_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1547470_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1517041
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9110	0.6005111265944691	No Hit
GAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	7243	0.47744260043070685	RNA PCR Primer, Index 35 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	5700	0.3757314403499971	TruSeq Adapter, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4709	0.31040690396633974	RNA PCR Primer, Index 35 (95% over 23bp)
AAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	4176	0.27527271840378736	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	4112	0.271053979424419	TruSeq Adapter, Index 10 (96% over 25bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3343	0.22036319387544567	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	2976	0.19617136254063008	RNA PCR Primer, Index 10 (95% over 24bp)
TAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	2369	0.156159260033183	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	2332	0.15372030156073568	No Hit
AGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1864	0.12287077277410433	TruSeq Adapter, Index 10 (95% over 23bp)
AGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	1844	0.12155241684305171	RNA PCR Primer, Index 10 (95% over 21bp)
TAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	1786	0.1177291846429991	RNA PCR Primer, Index 35 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1654	0.10902803549805179	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1623	0.10698458380492024	RNA PCR Primer, Index 35 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	240	0.0	45.000004	25
TATTTCG	40	6.8175723E-9	45.000004	15
AACGTCA	20	7.034E-4	45.000004	31
AACCGGT	20	7.034E-4	45.000004	3
CTCACGA	240	0.0	45.000004	24
GTACCGC	30	2.1659616E-6	45.000004	35
GGTACCC	20	7.034E-4	45.000004	33
AGATCGA	20	7.034E-4	45.000004	10
GTACACG	20	7.034E-4	45.000004	12
GGTCGAA	20	7.034E-4	45.000004	15
CACGGCG	30	2.1659616E-6	45.000004	10
CTATCCG	20	7.034E-4	45.000004	1
CGTGCAA	20	7.034E-4	45.000004	18
TTTCGAT	30	2.1659616E-6	45.000004	42
TAGCGTG	35	1.2122655E-7	45.000004	1
CGGTTGG	80	0.0	45.000004	2
GACGTAC	20	7.034E-4	45.000004	9
AGCTTCG	30	2.1659616E-6	45.000004	34
CAACCGT	40	6.8175723E-9	45.000004	32
ACCGAAT	30	2.1659616E-6	45.000004	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content