##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547470_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1517041
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.102107985215955	31.0	31.0	33.0	30.0	34.0
2	31.47841291039596	31.0	31.0	34.0	30.0	34.0
3	31.59380992339693	31.0	31.0	34.0	30.0	34.0
4	35.34675859123122	37.0	35.0	37.0	33.0	37.0
5	35.21174839704398	37.0	35.0	37.0	32.0	37.0
6	35.25940234970577	37.0	35.0	37.0	32.0	37.0
7	35.75265797035149	37.0	35.0	37.0	35.0	37.0
8	35.78311924331643	37.0	35.0	37.0	35.0	37.0
9	37.474188238814904	39.0	37.0	39.0	35.0	39.0
10	36.81337089768833	39.0	37.0	39.0	32.0	39.0
11	36.57194301274652	39.0	35.0	39.0	32.0	39.0
12	35.864597594923275	37.0	35.0	39.0	31.0	39.0
13	35.54373151417793	37.0	35.0	39.0	30.0	39.0
14	36.55769224430981	38.0	35.0	41.0	31.0	41.0
15	36.80189197259666	38.0	35.0	41.0	31.0	41.0
16	36.92703295428403	38.0	35.0	41.0	32.0	41.0
17	36.85893591537737	38.0	35.0	41.0	31.0	41.0
18	36.75002125848939	38.0	35.0	40.0	31.0	41.0
19	36.66726871587518	38.0	35.0	40.0	31.0	41.0
20	36.48873827404797	38.0	35.0	40.0	31.0	41.0
21	36.40265029092819	38.0	35.0	40.0	31.0	41.0
22	36.26467906931981	38.0	35.0	40.0	30.0	41.0
23	36.2310662665017	38.0	35.0	40.0	30.0	41.0
24	36.10649613293246	37.0	35.0	40.0	30.0	41.0
25	35.98733785046021	37.0	34.0	40.0	30.0	41.0
26	35.79400424906117	37.0	34.0	40.0	30.0	41.0
27	35.630219618322776	37.0	34.0	40.0	29.0	41.0
28	35.56168158935718	37.0	34.0	40.0	29.0	41.0
29	35.60739689962236	37.0	34.0	40.0	29.0	41.0
30	35.57397064416848	37.0	34.0	40.0	29.0	41.0
31	35.4181192202452	37.0	34.0	40.0	29.0	41.0
32	35.233652221660456	37.0	34.0	40.0	27.0	41.0
33	35.11674503194047	37.0	34.0	40.0	27.0	41.0
34	34.97996824080562	37.0	34.0	40.0	26.0	41.0
35	34.87679568317534	37.0	34.0	40.0	25.0	41.0
36	34.80027698658112	37.0	34.0	40.0	25.0	41.0
37	34.713338663885814	37.0	34.0	40.0	24.0	41.0
38	34.61595434797082	37.0	33.0	40.0	24.0	41.0
39	34.509580162961974	37.0	33.0	40.0	24.0	41.0
40	34.29389779181973	37.0	33.0	40.0	23.0	41.0
41	34.22055765137527	36.0	33.0	40.0	23.0	41.0
42	34.14817331898083	36.0	33.0	40.0	23.0	41.0
43	34.07674743134826	36.0	33.0	40.0	23.0	41.0
44	33.918167669825664	36.0	33.0	40.0	23.0	41.0
45	33.81076187130078	36.0	33.0	40.0	22.0	41.0
46	33.73521480302774	36.0	33.0	40.0	22.0	41.0
47	33.698070783848294	35.0	33.0	40.0	22.0	41.0
48	33.652323173862804	35.0	33.0	40.0	22.0	41.0
49	33.627157736672906	36.0	33.0	40.0	22.0	41.0
50	33.432679143147745	35.0	32.0	40.0	21.0	41.0
51	32.84610897134619	35.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	0.0
13	4.0
14	15.0
15	47.0
16	173.0
17	442.0
18	1017.0
19	1871.0
20	3081.0
21	4990.0
22	7248.0
23	10947.0
24	16012.0
25	21640.0
26	27636.0
27	31863.0
28	35701.0
29	41240.0
30	47574.0
31	56240.0
32	67300.0
33	83541.0
34	130573.0
35	190430.0
36	115374.0
37	133295.0
38	186819.0
39	301802.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.955365741598285	26.31649375330001	28.343202326107203	13.384938178994505
2	28.191064051663734	28.551832152196283	29.42880251753249	13.8283012786075
3	28.97911130945044	26.032915392530594	31.977118614460654	13.010854683558321
4	24.23579850511621	29.755491117247324	30.879916890842107	15.12879348679436
5	23.134378042518296	31.039701629685684	31.495655028440233	14.330265299355785
6	20.289761450086054	40.89414854311782	27.029724311999477	11.786365694796647
7	83.92535205047193	4.294148938624598	9.50079793492727	2.279701075976193
8	82.47904967631065	3.9569134914613384	7.629062101815311	5.934974730412692
9	76.9944912497421	6.949185948171473	12.107780870787275	3.9485419312991543
10	37.81315073224784	34.301182367516766	14.329474285797152	13.55619261443824
11	37.24968540731595	22.468871968522933	25.052256333217098	15.229186290944016
12	32.93490419837038	22.74157389286117	25.90707831891162	18.416443589856833
13	20.945709443581286	36.17384104978046	28.215387718591657	14.665061788046598
14	15.757056005737486	37.8108436093685	29.407313315856324	17.024787069037686
15	13.673328538912264	27.719422217329658	42.743867832181195	15.86338141157688
16	15.623572467718407	24.021433830727055	41.299806663102714	19.05518703845183
17	15.895021953922143	26.392760643911405	27.518900280216553	30.193317121949903
18	17.787126386168865	26.91331348328753	33.298243092968484	22.00131703757512
19	23.822296167341555	29.294989390530645	27.022868861158006	19.859845580969797
20	25.745184210578355	26.347541035476297	27.170195136453135	20.73707961749221
21	21.23238594078868	30.622771566490293	29.322213440506882	18.822629052214147
22	20.523769627847894	25.922107576525615	27.14850818138732	26.405614614239166
23	18.20735233919189	30.917753706063316	26.2536081753888	24.621285779355997
24	18.30161478826215	26.808438268972296	36.455837383432616	18.43410955933294
25	17.220826595985212	27.097355971262477	32.60920436560383	23.07261306714848
26	16.821826173452134	34.11278930496934	28.190602627087863	20.874781894490656
27	17.42945642207429	33.93467941868414	28.199831118605236	20.436033040636346
28	15.499449256809802	30.23484533377806	33.86632266365906	20.39938274575308
29	15.077245769890201	25.25429437965091	34.57124757999289	25.09721227046599
30	18.37379477548728	29.26229416344054	30.02621550768898	22.337695553383195
31	22.671832864108485	26.81885328082761	28.75176082914041	21.757553025923492
32	23.781756722461687	25.30979716434823	29.282662762575303	21.625783350614782
33	22.613957038735276	25.912022153653062	29.249374275316224	22.224646532295438
34	16.760390787065084	26.66124382927027	30.63028619529729	25.948079188367355
35	16.577139312648768	24.986272618867915	31.72109389265023	26.715494175833086
36	24.626427367487103	23.28308859154103	31.050512148320315	21.03997189265155
37	17.497088081337285	28.267858284647545	30.509524792012872	23.72552884200229
38	18.29587993996207	29.451346403953487	28.92110364848412	23.331670007600323
39	18.6085280490112	27.79456850539966	30.234252073609085	23.362651371980057
40	22.03361675788591	25.27617908810639	29.459520210726016	23.23068394328169
41	16.28420062476888	23.998626273119843	30.718220535898507	28.998952566212775
42	22.068223601076042	23.19113326535011	28.00636238572326	26.734280747850587
43	20.856258993659367	23.755521439433743	30.102218727114167	25.286000839792727
44	18.970680423271354	27.29616404566521	28.670286432601362	25.06286909846207
45	18.066024583383047	30.567862041961952	26.35307813038672	25.013035244268284
46	21.889454536825305	27.42285805063937	29.114110956790224	21.5735764557451
47	20.12200065785961	24.784827832603074	29.177985301649723	25.91518620788759
48	19.538430404979167	22.927857585918904	33.59401624610014	23.939695763001794
49	21.33409710086939	20.891129508035707	33.52598908005782	24.248784311037078
50	19.251556154382115	26.95879676290885	30.65724657408732	23.132400508621718
51	17.007714359730556	26.851548507917716	28.730798969836673	27.40993816251505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1533.0
1	1791.5
2	2050.0
3	7563.0
4	13076.0
5	9184.5
6	5293.0
7	6055.5
8	6818.0
9	8095.0
10	9372.0
11	10106.5
12	10841.0
13	11182.5
14	11524.0
15	11261.5
16	10999.0
17	10479.5
18	9960.0
19	9614.5
20	9269.0
21	9928.5
22	10588.0
23	10561.0
24	10534.0
25	10505.5
26	11494.5
27	12512.0
28	13542.0
29	14572.0
30	17007.0
31	19442.0
32	21911.5
33	24381.0
34	27917.5
35	31454.0
36	37344.0
37	43234.0
38	49022.0
39	54810.0
40	73323.5
41	91837.0
42	108659.5
43	125482.0
44	134462.0
45	143442.0
46	161685.0
47	179928.0
48	183322.0
49	186716.0
50	161776.5
51	136837.0
52	115677.0
53	94517.0
54	80928.5
55	67340.0
56	58440.5
57	49541.0
58	44730.5
59	39920.0
60	34720.0
61	29520.0
62	24547.0
63	19574.0
64	16114.5
65	12655.0
66	9986.0
67	7317.0
68	5711.5
69	4106.0
70	3275.0
71	2444.0
72	1853.0
73	1262.0
74	1053.5
75	731.0
76	617.0
77	432.0
78	247.0
79	161.0
80	75.0
81	58.5
82	42.0
83	29.5
84	17.0
85	10.0
86	3.0
87	2.0
88	1.0
89	2.5
90	4.0
91	6.5
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1517041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.647063810720738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56327657147608	16.014539207398474
2	9.621789803432168	3.973234160896123
3	3.692471759676972	2.2871610012400425
4	1.9114897966366131	1.5786660721879107
5	1.167985242115876	1.2057732911973305
6	0.7820290945031841	0.9687962769628444
7	0.530751814012965	0.7670926600107032
8	0.3904817884000124	0.6449841921615528
9	0.2946371999625517	0.5475053761775002
>10	2.0423408131795573	8.961065427454724
>50	0.7279193711449153	11.144871014893242
>100	1.259661760129748	46.88185627682089
>500	0.010002437132177282	1.2963520375752846
>1k	0.0041945704102678925	2.258939559592104
>5k	9.679777869848983E-4	1.4691634454313045
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9110	0.6005111265944691	No Hit
GAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	7243	0.47744260043070685	RNA PCR Primer, Index 35 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	5700	0.3757314403499971	TruSeq Adapter, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4709	0.31040690396633974	RNA PCR Primer, Index 35 (95% over 23bp)
AAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	4176	0.27527271840378736	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	4112	0.271053979424419	TruSeq Adapter, Index 10 (96% over 25bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3343	0.22036319387544567	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	2976	0.19617136254063008	RNA PCR Primer, Index 10 (95% over 24bp)
TAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	2369	0.156159260033183	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	2332	0.15372030156073568	No Hit
AGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1864	0.12287077277410433	TruSeq Adapter, Index 10 (95% over 23bp)
AGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	1844	0.12155241684305171	RNA PCR Primer, Index 10 (95% over 21bp)
TAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	1786	0.1177291846429991	RNA PCR Primer, Index 35 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1654	0.10902803549805179	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1623	0.10698458380492024	RNA PCR Primer, Index 35 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2974210980454714	0.0
2	0.0	0.0	0.0	1.098651915142702	0.0
3	0.0	0.0	0.0	1.5592195596559355	0.0
4	0.0	0.0	0.0	3.078756605787187	0.0
5	0.0	0.0	0.0	4.514841721482807	0.0
6	0.0	0.0	0.0	6.478401045192582	0.0
7	0.0	0.0	0.0	7.185501248812655	0.0
8	0.0	0.0	0.0	9.190456948757483	0.0
9	0.0	0.0	0.0	9.717469732195768	0.0
10	0.0	0.0	0.0	10.697469613543735	0.0
11	0.0	0.0	0.0	11.91273011078804	0.0
12	0.0	0.0	0.0	12.876909720963376	0.0
13	0.0	0.0	0.0	13.246115299454662	0.0
14	0.0	0.0	0.0	13.40339516202924	0.0
15	0.0	0.0	0.0	13.64643407791879	0.0
16	0.0	0.0	0.0	14.176808668981261	0.0
17	0.0	0.0	0.0	14.731506926971651	0.0
18	0.0	0.0	0.0	15.509073255106486	0.0
19	0.0	0.0	0.0	15.847429304811142	0.0
20	0.0	0.0	0.0	16.178534396895007	0.0
21	0.0	0.0	0.0	16.61075738889061	0.0
22	0.0	0.0	0.0	17.022282192768685	0.0
23	0.0	0.0	0.0	17.462481238147156	0.0
24	0.0	0.0	0.0	17.765703102289258	0.0
25	0.0	0.0	0.0	18.01329034614094	0.0
26	0.0	0.0	0.0	18.265360000158203	0.0
27	0.0	0.0	0.0	18.49304006945099	0.0
28	0.0	0.0	0.0	18.71650139976441	0.0
29	0.0	0.0	0.0	18.9793815724163	0.0
30	0.0	0.0	0.0	19.290447654348167	0.0
31	0.0	0.0	0.0	19.608435104918062	0.0
32	0.0	0.0	0.0	19.873292811466534	0.0
33	0.0	0.0	0.0	20.129976711242477	0.0
34	0.0	0.0	0.0	20.39002241864261	0.0
35	0.0	0.0	0.0	20.679401545508657	0.0
36	4.614245758684175E-4	0.0	0.0	20.956717715605578	0.0
37	4.614245758684175E-4	0.0	0.0	21.242735034847442	0.0
38	4.614245758684175E-4	0.0	0.0	21.515436959185678	0.0
39	4.614245758684175E-4	0.0	0.0	21.795455758941255	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	240	0.0	45.000004	25
TATTTCG	40	6.8175723E-9	45.000004	15
AACGTCA	20	7.034E-4	45.000004	31
AACCGGT	20	7.034E-4	45.000004	3
CTCACGA	240	0.0	45.000004	24
GTACCGC	30	2.1659616E-6	45.000004	35
GGTACCC	20	7.034E-4	45.000004	33
AGATCGA	20	7.034E-4	45.000004	10
GTACACG	20	7.034E-4	45.000004	12
GGTCGAA	20	7.034E-4	45.000004	15
CACGGCG	30	2.1659616E-6	45.000004	10
CTATCCG	20	7.034E-4	45.000004	1
CGTGCAA	20	7.034E-4	45.000004	18
TTTCGAT	30	2.1659616E-6	45.000004	42
TAGCGTG	35	1.2122655E-7	45.000004	1
CGGTTGG	80	0.0	45.000004	2
GACGTAC	20	7.034E-4	45.000004	9
AGCTTCG	30	2.1659616E-6	45.000004	34
CAACCGT	40	6.8175723E-9	45.000004	32
ACCGAAT	30	2.1659616E-6	45.000004	41
>>END_MODULE