FastQCFastQC Report
Thu 26 May 2016
SRR1547469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547469_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2747117
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148950.5422047914231538No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT33290.12118158782461759No Hit
GAGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT32380.11786902414422103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT302.1666092E-645.00000434
CGACTCG302.1666092E-645.00000414
CGAACTA207.035001E-445.024
TAGCGAT253.8922273E-545.01
TATATCG207.035001E-445.041
TCGATCA750.045.017
CCGTCGA207.035001E-445.017
GTCGTAC406.8212103E-945.044
GCGATCG207.035001E-445.09
ACTCGAT253.8922273E-545.010
CGTAAGT253.8922273E-545.020
CGTCGCG253.8922273E-545.029
ACCGTAA253.8922273E-545.039
TTACCCG1000.045.01
TCGACGA253.8922273E-545.026
TATCGTC253.8922273E-545.026
CCCGATA253.8922273E-545.013
TGCGTAG1600.043.593751
TAAACGG1100.042.9545442
TATAGCG2200.041.931821
Produced by FastQC (version 0.11.5)