##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547469_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2747117
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.324643617290416	31.0	31.0	33.0	30.0	34.0
2	31.741245094402604	31.0	31.0	34.0	30.0	34.0
3	31.876141787918023	31.0	31.0	34.0	30.0	34.0
4	35.62398652842234	37.0	35.0	37.0	33.0	37.0
5	35.49175626666065	37.0	35.0	37.0	33.0	37.0
6	35.545385944610295	37.0	35.0	37.0	33.0	37.0
7	35.947253065668484	37.0	35.0	37.0	35.0	37.0
8	35.97728746172806	37.0	35.0	37.0	35.0	37.0
9	37.708538806319496	39.0	37.0	39.0	35.0	39.0
10	37.067660751253044	39.0	37.0	39.0	33.0	39.0
11	36.900187360057835	39.0	37.0	39.0	32.0	39.0
12	36.44813271513372	39.0	35.0	39.0	32.0	39.0
13	36.278862895173376	39.0	35.0	39.0	32.0	39.0
14	37.42570411089153	39.0	36.0	41.0	32.0	41.0
15	37.55493413640555	39.0	36.0	41.0	32.0	41.0
16	37.5917603800639	39.0	36.0	41.0	32.0	41.0
17	37.5144131101806	39.0	35.0	41.0	32.0	41.0
18	37.41008082291362	39.0	36.0	41.0	32.0	41.0
19	37.36001196891141	39.0	36.0	41.0	32.0	41.0
20	37.23861379038461	39.0	35.0	41.0	32.0	41.0
21	37.18698475529073	39.0	35.0	41.0	32.0	41.0
22	37.097329309235825	39.0	35.0	41.0	32.0	41.0
23	37.00449962633554	39.0	35.0	41.0	31.0	41.0
24	36.93015477680783	39.0	35.0	41.0	31.0	41.0
25	36.851626996593154	38.0	35.0	41.0	31.0	41.0
26	36.71567101073598	38.0	35.0	40.0	31.0	41.0
27	36.641326161208276	38.0	35.0	40.0	31.0	41.0
28	36.55727550009701	38.0	35.0	40.0	31.0	41.0
29	36.61033039364541	38.0	35.0	40.0	31.0	41.0
30	36.59108003044646	38.0	35.0	40.0	31.0	41.0
31	36.49784410347284	38.0	35.0	40.0	30.0	41.0
32	36.43244208382825	38.0	35.0	41.0	30.0	41.0
33	36.328477454727995	38.0	35.0	41.0	30.0	41.0
34	36.220303321627725	38.0	35.0	41.0	30.0	41.0
35	36.09810393951186	38.0	35.0	41.0	30.0	41.0
36	36.04090142502121	38.0	35.0	41.0	29.0	41.0
37	35.98057563620333	38.0	35.0	41.0	29.0	41.0
38	35.88698733981843	38.0	35.0	41.0	29.0	41.0
39	35.81482077392408	38.0	35.0	41.0	29.0	41.0
40	35.68401127436509	38.0	35.0	40.0	28.0	41.0
41	35.618910297595626	38.0	35.0	40.0	28.0	41.0
42	35.57580619973594	38.0	35.0	40.0	27.0	41.0
43	35.53302644190255	38.0	34.0	40.0	27.0	41.0
44	35.40182271086379	38.0	34.0	40.0	27.0	41.0
45	35.300934033752476	38.0	34.0	40.0	27.0	41.0
46	35.26382458410035	38.0	34.0	40.0	27.0	41.0
47	35.22639807478167	38.0	34.0	40.0	27.0	41.0
48	35.17222091377979	38.0	34.0	40.0	26.0	41.0
49	35.10249072027147	38.0	34.0	40.0	26.0	41.0
50	34.97999393546034	37.0	34.0	40.0	26.0	41.0
51	34.416625866317304	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	7.0
14	16.0
15	52.0
16	155.0
17	367.0
18	800.0
19	1674.0
20	2905.0
21	4725.0
22	7491.0
23	11591.0
24	17575.0
25	25714.0
26	34506.0
27	40738.0
28	46222.0
29	55221.0
30	66391.0
31	81379.0
32	101117.0
33	128909.0
34	196890.0
35	281284.0
36	216167.0
37	272524.0
38	408055.0
39	744168.0
40	469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.264340033569738	25.34478873670106	30.47715113699198	12.913720092737222
2	29.183504015300404	26.967216904121667	30.476095484830097	13.373183595747834
3	27.610982713877856	26.35632191857864	32.44401312357646	13.58868224396704
4	25.561306635283465	28.650872896931585	31.35734662921164	14.430473838573313
5	22.99406978297612	32.85375176958244	29.918820348751073	14.23335809869037
6	21.592928149765736	41.683881683961765	26.602725693881986	10.120464472390509
7	89.46826800605872	3.0997951670787955	6.054055943012257	1.3778808838502328
8	88.84339472982039	3.052181614397931	5.583344284207771	2.521079371573908
9	82.23046197158695	7.271768912645511	7.766178142394373	2.731590973373176
10	30.369074196694207	45.250093097600136	12.955254545037578	11.425578160668074
11	33.300474643053064	24.061698136628326	26.494466744590785	16.143360475727828
12	32.6032709928263	22.408692458311748	26.54466482497833	18.44337172388362
13	23.30490474195311	30.995003125094417	27.17164940554043	18.52844272741205
14	18.39495733163167	32.659912191581206	28.418884233907765	20.526246242879353
15	17.11557971502488	28.177321897829614	36.3325624645765	18.374535922569006
16	20.580266512128897	25.79336810190465	35.12005495215529	18.506310433811155
17	20.035549996596433	26.03460282179463	27.857568498174633	26.07227868343431
18	21.016032444195133	26.230917722106483	32.07002104387982	20.683028789818565
19	24.626071623451057	28.13502300775686	27.113442929442026	20.125462439350052
20	25.899042523489168	26.70213900609257	28.011184088628188	19.387634381790072
21	23.17953694727964	28.915040749993537	28.737691186796923	19.1677311159299
22	23.43169220677532	25.757730740991374	27.201790094852168	23.608786957381138
23	19.338528355363096	29.67511030655047	28.219329573512887	22.76703176457355
24	19.603351440801394	26.20277913172246	34.23924062935797	19.954628798118172
25	19.43633998843151	29.72778370924864	30.061806613988413	20.77406968833144
26	19.6107046041359	33.131097073768615	26.924189978075198	20.334008344020294
27	18.893880384417557	32.372119571172256	28.210629543627007	20.523370500783184
28	17.427069906378215	29.584142211634962	32.972203222505634	20.016584659481193
29	18.42142143927616	26.770865602011128	32.278639752147434	22.529073206565283
30	20.022736563459073	29.569909108348863	30.125145743701488	20.282208584490576
31	23.352773107224774	28.682942881573663	28.140119259572856	19.824164751628707
32	23.834732921823132	28.031860310281655	28.425836977456733	19.707569790438484
33	23.503367348387417	28.11401916991522	27.43658169637478	20.946031785322578
34	20.3988035456808	28.230286514917275	28.363371490912108	23.007538448489818
35	20.243804686877187	27.49537060125215	30.76789230309448	21.492932408776184
36	24.265329798476003	28.14536111858359	27.14857066517371	20.4407384177667
37	20.668540873941662	31.549111304687788	28.331010291880542	19.451337529490008
38	20.430800726725508	30.565534704200804	27.612657196617402	21.39100737245629
39	20.478086663218203	30.1952556079701	27.88064723854135	21.446010490270346
40	23.095193979724925	27.77617407631346	28.178159139199387	20.950472804762228
41	18.408316791749314	27.079552854865664	29.40355288835532	25.108577465029704
42	20.971513044402553	27.391953091186146	27.810901392259595	23.825632472151714
43	21.018180150317587	27.109948356768204	29.362309650444445	22.50956184246976
44	20.200777760830718	27.41503183155286	29.48345483647038	22.90073557114604
45	19.291752044052004	30.81040960395935	26.5726942099663	23.325144142022346
46	20.733809298985083	30.542710776424887	27.362649643244175	21.360830281345862
47	20.450821716002633	27.75993887409965	29.189219097694057	22.600020312203668
48	20.708910468684078	25.677319167694712	31.486755023539224	22.127015340081986
49	20.72372600074915	26.054114185890153	30.998206483378755	22.223953329981942
50	19.298777591198334	28.47636267403245	29.810852613849352	22.41400712091986
51	18.819693518696145	28.182199738853498	28.922321109730674	24.075785632719686
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1954.0
1	3004.0
2	4054.0
3	13821.5
4	23589.0
5	16647.5
6	9706.0
7	10006.5
8	10307.0
9	11207.5
10	12108.0
11	12543.5
12	12979.0
13	12895.5
14	12812.0
15	12206.5
16	11601.0
17	11734.5
18	11868.0
19	11577.5
20	11287.0
21	12648.0
22	14009.0
23	14092.5
24	14176.0
25	17273.0
26	26098.0
27	31826.0
28	37801.0
29	43776.0
30	50084.5
31	56393.0
32	67369.5
33	78346.0
34	89128.0
35	99910.0
36	106221.0
37	112532.0
38	128094.5
39	143657.0
40	158507.5
41	173358.0
42	190239.0
43	207120.0
44	215802.0
45	224484.0
46	237453.0
47	250422.0
48	248536.0
49	246650.0
50	234671.0
51	222692.0
52	193022.5
53	163353.0
54	147162.5
55	130972.0
56	118045.0
57	105118.0
58	96552.0
59	87986.0
60	79536.5
61	71087.0
62	59399.0
63	47711.0
64	40692.0
65	33673.0
66	26637.5
67	19602.0
68	14746.0
69	9890.0
70	8109.5
71	6329.0
72	4861.0
73	3393.0
74	3025.5
75	2188.0
76	1718.0
77	1361.0
78	1004.0
79	616.0
80	228.0
81	217.0
82	206.0
83	141.0
84	76.0
85	89.0
86	102.0
87	54.5
88	7.0
89	7.0
90	7.0
91	5.5
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2747117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.146065687462322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.24041425839752	13.072231509138229
2	10.14097911790851	3.4775578818168604
3	3.636623807797814	1.8706137206727202
4	1.8515326061246506	1.2698599874836625
5	1.0460943731154544	0.896820141836115
6	0.6927411459187474	0.712667111539846
7	0.5089258889695316	0.6108253705625221
8	0.3569620609700929	0.4896395956260115
9	0.26628408656351005	0.41091519957694617
>10	2.512066118335029	9.88253624110578
>50	1.0663026485758935	13.423700871351294
>100	1.6664539845103477	51.0316969646889
>500	0.01160992282198326	1.3459340620581564
>1k	0.0027949814201070813	0.9559150985519448
>5k	0.0	0.0
>10k+	2.149985707774678E-4	0.549086243991023
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14895	0.5422047914231538	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3329	0.12118158782461759	No Hit
GAGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	3238	0.11786902414422103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06825337253564373	0.0
2	0.0	0.0	0.0	0.2743967584926306	0.0
3	0.0	0.0	0.0	0.4236077313052192	0.0
4	0.0	0.0	0.0	0.8524209198224902	0.0
5	0.0	0.0	0.0	1.36131806544825	0.0
6	3.6401798685676656E-5	0.0	0.0	2.0184433353220848	0.0
7	3.6401798685676656E-5	0.0	0.0	2.2841764657275245	0.0
8	3.6401798685676656E-5	0.0	0.0	3.0303405351865247	0.0
9	3.6401798685676656E-5	0.0	0.0	3.2629480287879984	0.0
10	3.6401798685676656E-5	0.0	0.0	3.7856414561156297	0.0
11	3.6401798685676656E-5	0.0	0.0	4.455325346536023	0.0
12	3.6401798685676656E-5	0.0	0.0	4.984207079640219	0.0
13	3.6401798685676656E-5	0.0	0.0	5.199523718865996	0.0
14	7.280359737135331E-5	0.0	0.0	5.28444911519968	0.0
15	7.280359737135331E-5	0.0	0.0	5.431075560305586	0.0
16	7.280359737135331E-5	0.0	0.0	5.744349439794519	0.0
17	7.280359737135331E-5	0.0	0.0	6.119251564458303	0.0
18	7.280359737135331E-5	0.0	0.0	6.565064392961785	0.0
19	7.280359737135331E-5	0.0	0.0	6.785477284003557	0.0
20	7.280359737135331E-5	0.0	0.0	7.026238780510623	0.0
21	7.280359737135331E-5	0.0	0.0	7.34267961648521	0.0
22	7.280359737135331E-5	0.0	0.0	7.672916734161668	0.0
23	7.280359737135331E-5	0.0	0.0	8.030964826033983	0.0
24	7.280359737135331E-5	0.0	0.0	8.301502993865933	0.0
25	7.280359737135331E-5	0.0	0.0	8.526611716938158	0.0
26	7.280359737135331E-5	0.0	0.0	8.737196122334797	0.0
27	7.280359737135331E-5	0.0	0.0	8.951020287814462	0.0
28	7.280359737135331E-5	0.0	0.0	9.174308920952402	0.0
29	7.280359737135331E-5	0.0	0.0	9.412704300544899	0.0
30	7.280359737135331E-5	0.0	0.0	9.700715331745972	0.0
31	7.280359737135331E-5	0.0	0.0	9.960624174361703	0.0
32	7.280359737135331E-5	0.0	0.0	10.203715385984653	0.0
33	7.280359737135331E-5	0.0	0.0	10.441710345791606	0.0
34	1.0920539605702997E-4	0.0	0.0	10.695758498818943	0.0
35	1.0920539605702997E-4	0.0	0.0	10.962219665198097	0.0
36	1.0920539605702997E-4	0.0	0.0	11.223766588754684	0.0
37	1.0920539605702997E-4	0.0	0.0	11.496852882494629	0.0
38	1.0920539605702997E-4	0.0	0.0	11.754250001001049	0.0
39	1.0920539605702997E-4	0.0	0.0	12.030175635038479	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	30	2.1666092E-6	45.000004	34
CGACTCG	30	2.1666092E-6	45.000004	14
CGAACTA	20	7.035001E-4	45.0	24
TAGCGAT	25	3.8922273E-5	45.0	1
TATATCG	20	7.035001E-4	45.0	41
TCGATCA	75	0.0	45.0	17
CCGTCGA	20	7.035001E-4	45.0	17
GTCGTAC	40	6.8212103E-9	45.0	44
GCGATCG	20	7.035001E-4	45.0	9
ACTCGAT	25	3.8922273E-5	45.0	10
CGTAAGT	25	3.8922273E-5	45.0	20
CGTCGCG	25	3.8922273E-5	45.0	29
ACCGTAA	25	3.8922273E-5	45.0	39
TTACCCG	100	0.0	45.0	1
TCGACGA	25	3.8922273E-5	45.0	26
TATCGTC	25	3.8922273E-5	45.0	26
CCCGATA	25	3.8922273E-5	45.0	13
TGCGTAG	160	0.0	43.59375	1
TAAACGG	110	0.0	42.954544	2
TATAGCG	220	0.0	41.93182	1
>>END_MODULE