FastQCFastQC Report
Thu 26 May 2016
SRR1547468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547468_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3370428
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530971.5753785572633505No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT35870.1064256527657615RNA PCR Primer, Index 30 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC207.0352305E-445.028
TAGCCGT253.892416E-545.01
TCGAGTA207.0352305E-445.013
CCGCATC207.0352305E-445.020
CGTTTTT224600.043.908061
GCGTAAG2850.041.052631
TACGGGA8900.040.449444
AGGGCGA34400.040.029076
CTATGCG1350.040.01
TCTCACG3550.039.92957723
GACGGGT4400.039.8863644
GGGCGAT64550.039.4577877
ACGCGAG403.460882E-739.3751
CTCGAAT403.460882E-739.37532
GCGATGT8250.039.09
GGCGATT17950.038.9832888
TAGGGCG13350.038.9325835
TTTAGCG2450.038.571431
GGACCGA15750.038.571438
TAACCGA356.2518848E-638.57142638
Produced by FastQC (version 0.11.5)