FastQCFastQC Report
Thu 26 May 2016
SRR1547467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547467_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3531117
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338140.9576006685703137No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA207.035276E-445.035
GCGCGAA207.035276E-445.023
CGGCGAA302.1667856E-644.99999621
CGTTTTT165550.043.17881
CGACGGT6050.041.65289328
ACGGGAT25600.039.9023445
GGGCGAT64200.038.7266357
TAAGGGA74100.038.6842164
TACGGGA20500.038.6341484
CTCGCAC356.2519703E-638.57142617
TCGACCG356.2519703E-638.5714261
AGGGCGA32750.038.335886
TCACGAC6750.038.33333225
GGCGATT17350.038.2564858
AAGGGAC49400.038.2135625
TTAGGGA76700.038.1942634
TAGGGAC52450.037.964735
CGTACGC950.037.89473721
CGGGATT25150.037.753486
TTACGCG1550.037.7419361
Produced by FastQC (version 0.11.5)