FastQCFastQC Report
Thu 26 May 2016
SRR1547466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547466_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4565511
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253430.5550966803058847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152150.041.8501471
TAGTACG1950.039.230771
CGACGGT6550.039.16030528
CGCGTAA1100.038.86363631
TAGGGAC70250.038.6583635
TACGGGA27300.038.6538434
TTAGGGA94800.037.998424
TAGGGAT97550.037.988215
TAAGGGA94000.037.9388284
GGGCGAT83400.037.607917
CGTTAGG8200.037.5914652
GTAGGGA97350.037.4653324
ATAGGGA101700.037.4336284
GCGGGAT38400.037.148445
ATACCGG2000.037.1252
CGGTCTA7000.036.96428731
AGGGCGA44200.036.9061056
AGGGATC68150.036.8451966
TAGGGAG107850.036.8219765
TATGGGA82100.036.8057254
Produced by FastQC (version 0.11.5)