##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547466_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4565511
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272166686270168	31.0	31.0	33.0	30.0	34.0
2	31.692706687159443	31.0	31.0	34.0	30.0	34.0
3	31.828625317078416	31.0	31.0	34.0	30.0	34.0
4	35.57832606251524	37.0	35.0	37.0	33.0	37.0
5	35.45605409777788	37.0	35.0	37.0	33.0	37.0
6	35.50483615087117	37.0	35.0	37.0	33.0	37.0
7	35.93327997676492	37.0	35.0	37.0	35.0	37.0
8	35.966899871668254	37.0	35.0	37.0	35.0	37.0
9	37.69658839941466	39.0	37.0	39.0	35.0	39.0
10	36.99299114600753	39.0	37.0	39.0	33.0	39.0
11	36.84691045536852	39.0	37.0	39.0	32.0	39.0
12	36.53427119111092	39.0	35.0	39.0	32.0	39.0
13	36.47246058546349	39.0	35.0	39.0	32.0	39.0
14	37.56993138336541	40.0	36.0	41.0	32.0	41.0
15	37.64425734600135	40.0	36.0	41.0	32.0	41.0
16	37.65297159507446	40.0	36.0	41.0	32.0	41.0
17	37.58817906692153	40.0	36.0	41.0	32.0	41.0
18	37.47441765007247	39.0	36.0	41.0	32.0	41.0
19	37.423460594005796	39.0	36.0	41.0	32.0	41.0
20	37.32862498852812	39.0	35.0	41.0	32.0	41.0
21	37.27651121637863	39.0	35.0	41.0	32.0	41.0
22	37.19403019727693	39.0	35.0	41.0	31.0	41.0
23	37.095838998088055	39.0	35.0	41.0	31.0	41.0
24	37.0315695220097	39.0	35.0	41.0	31.0	41.0
25	36.929931392126754	39.0	35.0	41.0	31.0	41.0
26	36.796850779682714	39.0	35.0	41.0	31.0	41.0
27	36.707709827005125	39.0	35.0	40.0	30.0	41.0
28	36.6000594457006	38.0	35.0	40.0	30.0	41.0
29	36.654132691827925	39.0	35.0	40.0	30.0	41.0
30	36.59945097054853	39.0	35.0	40.0	30.0	41.0
31	36.513004130315316	39.0	35.0	40.0	30.0	41.0
32	36.48578308101766	39.0	35.0	41.0	30.0	41.0
33	36.38694770421098	39.0	35.0	41.0	30.0	41.0
34	36.25820526990297	39.0	35.0	41.0	30.0	41.0
35	36.14419371676029	39.0	35.0	41.0	29.0	41.0
36	36.11087762136593	39.0	35.0	41.0	29.0	41.0
37	36.06916947522413	39.0	35.0	41.0	29.0	41.0
38	35.959694763631056	39.0	35.0	41.0	28.0	41.0
39	35.87728142589077	39.0	35.0	41.0	28.0	41.0
40	35.79228086406976	38.0	35.0	41.0	27.0	41.0
41	35.696368489748465	38.0	35.0	41.0	27.0	41.0
42	35.65150297524198	38.0	35.0	40.0	27.0	41.0
43	35.62931772587997	38.0	35.0	40.0	27.0	41.0
44	35.50288434306697	38.0	34.0	40.0	27.0	41.0
45	35.37206415667381	38.0	34.0	40.0	26.0	41.0
46	35.34152431129834	38.0	34.0	40.0	26.0	41.0
47	35.28462399937269	38.0	34.0	40.0	26.0	41.0
48	35.20696719381467	38.0	34.0	40.0	26.0	41.0
49	35.12355856770469	38.0	34.0	40.0	26.0	41.0
50	35.02770883697356	38.0	34.0	40.0	26.0	41.0
51	34.4375867235891	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	1.0
12	3.0
13	19.0
14	34.0
15	103.0
16	267.0
17	693.0
18	1526.0
19	3053.0
20	5179.0
21	8607.0
22	13370.0
23	20043.0
24	29431.0
25	43768.0
26	60551.0
27	71826.0
28	81850.0
29	94029.0
30	112441.0
31	136094.0
32	165788.0
33	206878.0
34	291526.0
35	370016.0
36	371392.0
37	482518.0
38	723261.0
39	1270636.0
40	600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.610196536597986	25.849198479644446	29.58085086203932	12.959754121718248
2	31.084362736175642	25.487815055094597	29.781879837766244	13.645942370963512
3	28.602099524018232	26.023943431523875	31.86453827402891	13.509418770428983
4	25.321415280786752	28.626828409788086	31.318443871890793	14.733312437534373
5	22.936183923333004	32.730421632978214	30.02102064807203	14.312373795616745
6	21.70771245540751	41.65789984954586	26.447926639537172	10.186461055509449
7	90.70463306298025	2.42375935574353	5.692133914473101	1.1794736668031245
8	90.83745499682291	2.1824939201767335	5.418911486578392	1.5611395964219559
9	84.48850522975413	6.179023552894736	6.973326753566029	2.359144463785105
10	26.113440532724596	49.374232150574166	12.833284160305386	11.679043156395856
11	30.807285318116634	24.710443146451734	27.344649919800872	17.137621615630756
12	28.92412262285646	23.228396558457533	28.240234225697847	19.60724659298817
13	25.987890512146393	24.7057558288656	29.689688624121157	19.616665034866855
14	21.671287179025526	27.26581975161159	30.21427393341074	20.84861913595214
15	20.843778494893563	26.912540567748056	32.686133052795185	19.557547884563196
16	24.719773974917594	26.19246783109273	30.55469584894221	18.533062345047465
17	23.743278682276745	25.931883637998027	28.680601141909417	21.64423653781581
18	23.643399391656267	25.6975396620444	30.384813441474567	20.27424750482476
19	24.67336076947356	26.559217577178106	28.21716999477167	20.550251658576663
20	26.09497600597173	27.051933507552604	28.302746395748475	18.550344090727194
21	25.885295205728344	25.86453082688882	29.678233170394293	18.57194079698855
22	25.206751226752054	23.441713315333157	29.772089038883053	21.579446419031736
23	21.842921854749665	26.94298622870474	29.796511277708014	21.41758063883758
24	21.231248813112046	26.90465536059381	31.31903526242736	20.545060563866784
25	21.97083743747414	28.73367296672815	28.932927770845367	20.362561824952344
26	22.125343690991	29.2988013828025	27.952906038338316	20.62294888786819
27	20.942715941326174	28.255522766235806	29.753274058478883	21.048487233959136
28	19.970557512620164	28.041373681938342	31.031685171714624	20.956383633726873
29	21.253261683084325	26.25202304846051	30.654137072498568	21.8405781959566
30	22.603537698189754	26.757705763933103	29.836156347011322	20.80260019086582
31	23.08773322416702	27.42937209000263	28.970973895364615	20.51192079046573
32	24.06298002567511	27.087066486095424	28.505461929672276	20.34449155855719
33	23.02239552155279	27.540684930996772	28.39489380268715	21.04202574476329
34	21.807328905789518	27.809088621186106	29.335117142418447	21.04846533060593
35	21.717853707942	28.037693918599693	28.824571882534066	21.419880490924236
36	23.09154440762491	28.322108959982796	27.983745959652712	20.60260067273959
37	21.616857346308006	29.10631471482601	28.696765816575624	20.580062122290364
38	22.010000633006907	28.769046882156236	28.0233472222496	21.197605262587256
39	21.98279666832475	27.85975107715215	28.20626212487496	21.951190129648136
40	22.914652927131268	27.65806500082904	28.553693113432427	20.87358895860726
41	20.735488316641884	27.33306304595477	28.722830806891057	23.20861783051229
42	21.789411962866804	27.422866794100376	28.419754108576235	22.36796713445658
43	22.093474312075912	27.217632374557855	28.763264396909786	21.92562891645645
44	21.984176579576744	27.659379202021416	28.848380827469256	21.50806339093258
45	21.292249651791444	27.931243622017337	28.25298197726388	22.523524748927336
46	22.003363916985418	28.017674253769183	28.192747755946705	21.786214073298694
47	21.324798034655924	27.80236429175179	29.385472951439606	21.48736472215268
48	21.396619129819204	28.030487715394838	29.248270346955685	21.324622807830274
49	21.91693328523357	27.09028627901674	29.239881362677693	21.752899073071998
50	21.247544907897495	27.866212566347993	29.350909460080153	21.535333065674354
51	21.134589315412885	27.530324644930214	28.705921418215834	22.62916462144106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1631.0
1	3064.0
2	4497.0
3	23193.5
4	41890.0
5	29246.0
6	16602.0
7	16836.5
8	17071.0
9	17692.5
10	18314.0
11	18707.5
12	19101.0
13	19055.0
14	19009.0
15	18763.5
16	18518.0
17	18290.0
18	18062.0
19	17567.0
20	17072.0
21	17894.5
22	18717.0
23	21455.0
24	24193.0
25	28189.0
26	39845.5
27	47506.0
28	55092.0
29	62678.0
30	79394.0
31	96110.0
32	111676.5
33	127243.0
34	141649.5
35	156056.0
36	172932.0
37	189808.0
38	206919.0
39	224030.0
40	239072.5
41	254115.0
42	277293.0
43	300471.0
44	316098.0
45	331725.0
46	348300.0
47	364875.0
48	375660.0
49	386445.0
50	373589.5
51	360734.0
52	337609.0
53	314484.0
54	290148.0
55	265812.0
56	242359.5
57	218907.0
58	199768.5
59	180630.0
60	157409.5
61	134189.0
62	115866.5
63	97544.0
64	83753.5
65	69963.0
66	57074.0
67	44185.0
68	35633.0
69	27081.0
70	21544.5
71	16008.0
72	13990.0
73	11972.0
74	9576.0
75	5801.0
76	4422.0
77	3559.0
78	2696.0
79	1774.5
80	853.0
81	692.5
82	532.0
83	317.0
84	102.0
85	138.5
86	175.0
87	115.0
88	55.0
89	45.0
90	35.0
91	28.5
92	22.0
93	12.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4565511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.993426797953592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38220920869712	13.92371116860622
2	8.612440842880247	3.099346477161429
3	2.7740677259631874	1.4974495367905258
4	1.371546211510553	0.9871526542730285
5	0.81738841446423	0.7353809300578739
6	0.5938650857110453	0.6411400768561277
7	0.4065747752305561	0.5120971419203811
8	0.35087584496703117	0.5050767065267512
9	0.2999612461723723	0.48575976527029663
>10	4.218944092304939	19.092296942932265
>50	1.922797938579832	24.91416976306511
>100	1.2442921061194583	32.27943994054939
>500	0.0036852493167038457	0.44392511280217206
>1k	0.0012284164389008768	0.3228867779233462
>5k	0.0	0.0
>10k+	1.2284164389008768E-4	0.5601670052650166
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25343	0.5550966803058847	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3142011923747418E-4	0.0	0.0	0.04536184449013484	0.0
2	1.3142011923747418E-4	0.0	0.0	0.141386144946316	0.0
3	1.3142011923747418E-4	0.0	0.0	0.22343610605691236	0.0
4	1.3142011923747418E-4	0.0	0.0	0.3661364521956031	0.0
5	1.3142011923747418E-4	0.0	0.0	0.6171926866455912	0.0
6	1.3142011923747418E-4	0.0	0.0	0.986067057991975	0.0
7	1.3142011923747418E-4	0.0	0.0	1.157307473358404	0.0
8	1.5332347244371988E-4	0.0	0.0	1.6240679301834997	0.0
9	1.5332347244371988E-4	0.0	0.0	1.8074208998729824	0.0
10	1.5332347244371988E-4	0.0	0.0	2.1596706261358256	0.0
11	1.5332347244371988E-4	0.0	0.0	2.5107156679723257	0.0
12	1.5332347244371988E-4	0.0	0.0	2.823605068523545	0.0
13	1.5332347244371988E-4	0.0	0.0	2.969349980757904	0.0
14	1.5332347244371988E-4	0.0	0.0	3.0416529496917213	0.0
15	1.5332347244371988E-4	0.0	0.0	3.1394952284640207	0.0
16	1.5332347244371988E-4	0.0	0.0	3.313714499866499	0.0
17	1.5332347244371988E-4	0.0	0.0	3.5205040574866646	0.0
18	1.5332347244371988E-4	0.0	0.0	3.758440183366112	0.0
19	1.5332347244371988E-4	0.0	0.0	3.897723606404628	0.0
20	1.5332347244371988E-4	0.0	0.0	4.066248005973483	0.0
21	1.7522682564996558E-4	0.0	0.0	4.269839674025536	0.0
22	1.7522682564996558E-4	0.0	0.0	4.500525789993716	0.0
23	1.7522682564996558E-4	0.0	0.0	4.73252610715427	0.0
24	1.7522682564996558E-4	0.0	0.0	4.916054303669403	0.0
25	1.9713017885621129E-4	0.0	0.0	5.083636859050389	0.0
26	1.9713017885621129E-4	0.0	0.0	5.242107619497577	0.0
27	1.9713017885621129E-4	0.0	0.0	5.4124499973825495	0.0
28	1.9713017885621129E-4	0.0	0.0	5.587370176087627	0.0
29	1.9713017885621129E-4	0.0	0.0	5.781696725733439	0.0
30	1.9713017885621129E-4	0.0	0.0	6.004497634547371	0.0
31	1.9713017885621129E-4	0.0	0.0	6.198473730541882	0.0
32	2.4093688526870266E-4	0.0	0.0	6.390960398518369	0.0
33	2.4093688526870266E-4	0.0	0.0	6.586338309118081	0.0
34	2.4093688526870266E-4	0.0	0.0	6.787432994904623	0.0
35	2.4093688526870266E-4	0.0	0.0	7.0030715072201115	0.0
36	2.4093688526870266E-4	0.0	0.0	7.212401853812202	0.0
37	2.4093688526870266E-4	0.0	0.0	7.433625721195283	0.0
38	2.4093688526870266E-4	0.0	0.0	7.670160032469531	0.0
39	2.4093688526870266E-4	0.0	0.0	7.974791868861996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15215	0.0	41.850147	1
TAGTACG	195	0.0	39.23077	1
CGACGGT	655	0.0	39.160305	28
CGCGTAA	110	0.0	38.863636	31
TAGGGAC	7025	0.0	38.658363	5
TACGGGA	2730	0.0	38.653843	4
TTAGGGA	9480	0.0	37.99842	4
TAGGGAT	9755	0.0	37.98821	5
TAAGGGA	9400	0.0	37.938828	4
GGGCGAT	8340	0.0	37.60791	7
CGTTAGG	820	0.0	37.591465	2
GTAGGGA	9735	0.0	37.465332	4
ATAGGGA	10170	0.0	37.433628	4
GCGGGAT	3840	0.0	37.14844	5
ATACCGG	200	0.0	37.125	2
CGGTCTA	700	0.0	36.964287	31
AGGGCGA	4420	0.0	36.906105	6
AGGGATC	6815	0.0	36.845196	6
TAGGGAG	10785	0.0	36.821976	5
TATGGGA	8210	0.0	36.805725	4
>>END_MODULE