FastQCFastQC Report
Thu 26 May 2016
SRR1547465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547465_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1872793
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110830.5917899095094866No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53210.2841210961382278No Hit
GAGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT24860.13274291392588503No Hit
AAGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT18790.1003314301153411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGA600.045.00000414
CGCATTA302.1662363E-645.00000444
GTAGCGT302.1662363E-645.00000438
CGCTCGA302.1662363E-645.00000419
CGTAGCG302.1662363E-645.0000044
ACGTACA302.1662363E-645.00000425
CCAACCG302.1662363E-645.00000425
ATACGTC600.045.00000426
AACCGTA253.8917497E-545.012
TACCCGT253.8917497E-545.044
CGACGTG253.8917497E-545.040
GCGCGAC351.2124838E-745.09
CGTCTAG207.034425E-445.011
CGCGACA351.2124838E-745.024
TAACGCG253.8917497E-545.01
CTACGCT207.034425E-445.031
AACGCTA253.8917497E-545.043
ACTACGC207.034425E-445.026
ACTAACG406.8193913E-945.021
TCCCGAA453.8562575E-1045.036
Produced by FastQC (version 0.11.5)