##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547465_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1872793
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.320474820228398	31.0	31.0	33.0	30.0	34.0
2	31.726533044495575	31.0	31.0	34.0	30.0	34.0
3	31.865122306629722	31.0	31.0	34.0	30.0	34.0
4	35.60221124278017	37.0	35.0	37.0	33.0	37.0
5	35.48409728144007	37.0	35.0	37.0	33.0	37.0
6	35.52807865044348	37.0	35.0	37.0	33.0	37.0
7	35.932926383214806	37.0	35.0	37.0	35.0	37.0
8	35.97078961743236	37.0	35.0	37.0	35.0	37.0
9	37.72217057624628	39.0	37.0	39.0	35.0	39.0
10	37.06406527576726	39.0	37.0	39.0	33.0	39.0
11	36.85329184805796	39.0	37.0	39.0	32.0	39.0
12	36.22111787047474	38.0	35.0	39.0	32.0	39.0
13	35.932858570060866	38.0	35.0	39.0	31.0	39.0
14	37.00869823840649	39.0	35.0	41.0	31.0	41.0
15	37.221752217143056	39.0	35.0	41.0	32.0	41.0
16	37.31252733217179	39.0	35.0	41.0	32.0	41.0
17	37.258137444981905	39.0	35.0	41.0	32.0	41.0
18	37.16350392168275	39.0	35.0	41.0	32.0	41.0
19	37.09398102192821	38.0	35.0	41.0	32.0	41.0
20	36.967482791744736	38.0	35.0	41.0	32.0	41.0
21	36.88406139920429	38.0	35.0	41.0	32.0	41.0
22	36.8002315258547	38.0	35.0	41.0	31.0	41.0
23	36.730347667894954	38.0	35.0	40.0	31.0	41.0
24	36.6558482437728	38.0	35.0	40.0	31.0	41.0
25	36.56286466256548	38.0	35.0	40.0	31.0	41.0
26	36.42682773803619	38.0	35.0	40.0	31.0	41.0
27	36.34936002003425	38.0	35.0	40.0	31.0	41.0
28	36.27870405325095	38.0	35.0	40.0	30.0	41.0
29	36.346321777153165	38.0	35.0	40.0	31.0	41.0
30	36.29566481720083	38.0	35.0	40.0	30.0	41.0
31	36.19264542317277	38.0	35.0	40.0	30.0	41.0
32	36.04538195091502	38.0	35.0	40.0	30.0	41.0
33	35.90791988222938	38.0	35.0	40.0	30.0	41.0
34	35.777633192776776	38.0	35.0	40.0	29.0	41.0
35	35.65816617212901	38.0	35.0	40.0	29.0	41.0
36	35.572294428695535	38.0	35.0	40.0	28.0	41.0
37	35.501718556188536	38.0	34.0	40.0	27.0	41.0
38	35.39851120759208	38.0	34.0	40.0	27.0	41.0
39	35.32384785718443	38.0	34.0	40.0	27.0	41.0
40	35.162741424172346	38.0	34.0	40.0	26.0	41.0
41	35.116959001875806	38.0	34.0	40.0	26.0	41.0
42	35.056342051684304	38.0	34.0	40.0	26.0	41.0
43	35.00959849807213	38.0	34.0	40.0	26.0	41.0
44	34.895602984419526	37.0	34.0	40.0	25.0	41.0
45	34.77897824265683	37.0	34.0	40.0	24.0	41.0
46	34.745449176710935	37.0	34.0	40.0	25.0	41.0
47	34.70062788573003	37.0	34.0	40.0	25.0	41.0
48	34.628956323523205	37.0	34.0	40.0	24.0	41.0
49	34.59013622968475	37.0	34.0	40.0	24.0	41.0
50	34.450806362475724	36.0	34.0	40.0	24.0	41.0
51	33.8949024264828	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	4.0
12	2.0
13	3.0
14	10.0
15	48.0
16	116.0
17	307.0
18	686.0
19	1436.0
20	2471.0
21	4068.0
22	6272.0
23	9489.0
24	14098.0
25	20670.0
26	26392.0
27	31541.0
28	35440.0
29	41014.0
30	48369.0
31	58215.0
32	72592.0
33	92647.0
34	149893.0
35	225883.0
36	139454.0
37	174331.0
38	258811.0
39	458265.0
40	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.548338230653364	23.947814841255813	30.639638230172793	12.864208697918029
2	29.161738643833036	27.43042076727113	30.4753915675678	12.932449021328038
3	28.155327363995912	26.654894587922957	31.80906806037827	13.38070998770286
4	25.072445272915907	28.59157418892531	31.48554058029905	14.850439957859733
5	23.163585083882733	32.559498033151556	29.807405303202223	14.469511579763486
6	21.11461330750382	41.52909584775253	26.50527847978928	10.851012364954375
7	88.52793661659351	3.7148259311093113	6.240091670569037	1.5171457817281462
8	88.71023118945874	3.2124212339537794	5.567993900019917	2.509353676567565
9	83.38401521150496	6.631645889321456	7.5869570208773744	2.3973818782962133
10	35.653646719098155	40.02914363733739	12.776318578721726	11.540891064842725
11	37.44674398078164	23.739836703789475	24.163695613984036	14.649723701444847
12	35.29818832086622	22.522296911618103	24.988559867534747	17.190954899980937
13	23.27528990123308	33.67072602257697	26.038061868022787	17.01592220816716
14	17.063818585396252	36.902530071396036	27.61501137605704	18.418639967150668
15	16.761649578997787	26.892721192358156	40.13833883403024	16.20729039461382
16	19.027997221262574	24.78640191414641	38.675657160188024	17.509943704402996
17	18.423659208465644	24.559040961814787	28.377188509354745	28.640111320364824
18	20.332199020393602	26.112869922089626	33.155666429765596	20.399264627751172
19	24.819454152167378	27.781340489845913	26.826670112500423	20.572535245486286
20	27.479331672000058	25.691307047815748	27.954237334291616	18.87512394589258
21	22.996775404436047	28.516338965384858	29.347984534329207	19.13890109584989
22	23.364194547929216	25.686875164526988	27.089325942589493	23.859604344954302
23	19.20489877952342	30.24172986550035	27.533422006596563	23.01994934837967
24	19.336039807923246	26.262379237854905	35.15247013417927	19.249110820042578
25	18.38815074597139	27.64272399565782	33.21434883620347	20.75477642216732
26	18.19266731560829	33.829472878209174	27.711551677094054	20.26630812908848
27	18.45868710530208	34.31564513536733	28.182559417938872	19.043108341391708
28	16.65330872125216	30.605304483730983	34.3884241344345	18.35296266058235
29	17.326901584958936	26.534112419258292	33.728340505330806	22.410645490451962
30	18.802184758272805	31.12025728417396	30.637715967541528	19.43984199001171
31	24.300069468435648	29.791012674652244	25.993849827503627	19.915068029408484
32	25.03036907976482	29.12307980647087	28.00528408638862	17.84126702737569
33	25.33205752050547	30.317232069961815	25.52529831113209	18.825412098400623
34	19.673557088263358	29.404851470504216	28.82112438480921	22.100467056423216
35	20.470655326029092	28.514737079858797	28.894063572428983	22.120544021683124
36	26.15569366181954	27.389305705435678	27.34552083439013	19.109479798354652
37	20.888373675040434	31.121004830752785	29.058523819770794	18.932097674435987
38	20.57605939364361	31.348686160189622	26.01456754697396	22.06068689919281
39	21.04263525119968	30.65992878016951	28.588263625504794	19.709172343126017
40	23.496670480934092	27.321118778209875	28.020181621780942	21.162029119075093
41	19.12736752006228	27.10048574508768	28.91878600571446	24.853360729135574
42	21.951972268157775	26.09177842932988	27.768899178926876	24.187350123585468
43	20.952876265556313	26.77546317185081	28.322671005284622	23.948989557308256
44	20.260007379352658	28.50037350630849	28.832764752965222	22.406854361373625
45	19.4283084142241	32.93033453243364	24.766752118360117	22.874604934982138
46	21.76487203871437	31.19704099705627	27.36549100728164	19.672595956947724
47	21.47423660810351	28.51553802262183	27.660451528812846	22.34977384046181
48	21.74677073226993	26.534753173468715	30.884993696580455	20.833482397680896
49	21.470231894288368	25.784429993063835	30.494293816775265	22.25104429587253
50	19.7710051244318	30.49295891217022	28.437899970792284	21.298135992605697
51	18.696513709737275	30.024087018693468	27.012916002996594	24.26648326857266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1511.0
1	2126.5
2	2742.0
3	9592.0
4	16442.0
5	11294.5
6	6147.0
7	6805.5
8	7464.0
9	8470.5
10	9477.0
11	10128.0
12	10779.0
13	11108.5
14	11438.0
15	11098.5
16	10759.0
17	10507.5
18	10256.0
19	10373.5
20	10491.0
21	10011.5
22	9532.0
23	10840.0
24	12148.0
25	13969.5
26	19366.0
27	22941.0
28	24086.0
29	25231.0
30	31566.5
31	37902.0
32	42836.0
33	47770.0
34	49104.0
35	50438.0
36	59059.0
37	67680.0
38	73822.5
39	79965.0
40	100790.5
41	121616.0
42	137726.5
43	153837.0
44	160752.0
45	167667.0
46	173607.5
47	179548.0
48	176006.0
49	172464.0
50	158774.5
51	145085.0
52	129818.5
53	114552.0
54	104526.5
55	94501.0
56	82567.0
57	70633.0
58	65343.0
59	60053.0
60	54957.5
61	49862.0
62	40776.0
63	31690.0
64	24800.0
65	17910.0
66	14223.0
67	10536.0
68	8764.5
69	6993.0
70	5474.0
71	3955.0
72	2940.5
73	1926.0
74	1703.0
75	1155.0
76	830.0
77	665.5
78	501.0
79	300.5
80	100.0
81	107.0
82	114.0
83	71.5
84	29.0
85	16.5
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1872793.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.70297028384886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14517617334093	13.219595257332962
2	8.91181824393023	2.977076706068575
3	3.126297801129901	1.5665537781220433
4	1.5686220598456453	1.048025906087664
5	0.8710941974440624	0.7274930247170672
6	0.5867748069725078	0.588052929850377
7	0.40535822166513463	0.4739480431550593
8	0.28854614135656403	0.3855662099678353
9	0.22997032468458842	0.34570687494363783
>10	1.9054919729434603	7.327092393374629
>50	1.047212774490913	12.99718242226778
>100	1.900700744881884	55.73396316308163
>500	0.00905557611990654	0.9550674046371027
>1k	0.0032341343285380497	0.7685375546251165
>5k	3.23413432853805E-4	0.2874385554340511
>10k+	3.23413432853805E-4	0.5986997763344463
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11083	0.5917899095094866	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5321	0.2841210961382278	No Hit
GAGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	2486	0.13274291392588503	No Hit
AAGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	1879	0.1003314301153411	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09045313603799246	0.0
2	0.0	0.0	0.0	0.3033971186351081	0.0
3	0.0	0.0	0.0	0.4409456891391627	0.0
4	0.0	0.0	0.0	0.8847747722252273	0.0
5	0.0	0.0	0.0	1.3327687576790388	0.0
6	0.0	0.0	0.0	1.951096570736862	0.0
7	0.0	0.0	0.0	2.19271430425039	0.0
8	0.0	0.0	0.0	2.800683257573047	0.0
9	0.0	0.0	0.0	2.9810021716228117	0.0
10	0.0	0.0	0.0	3.360435456561403	0.0
11	0.0	0.0	0.0	3.884572400687102	0.0
12	0.0	0.0	0.0	4.298606412988515	0.0
13	0.0	0.0	0.0	4.462746283225108	0.0
14	0.0	0.0	0.0	4.528797363082839	0.0
15	0.0	0.0	0.0	4.635749920039214	0.0
16	0.0	0.0	0.0	4.881212178815278	0.0
17	0.0	0.0	0.0	5.144295178377963	0.0
18	0.0	0.0	0.0	5.495054712400142	0.0
19	0.0	0.0	0.0	5.673130986713428	0.0
20	0.0	0.0	0.0	5.847095754843167	0.0
21	0.0	0.0	0.0	6.077820666779511	0.0
22	0.0	0.0	0.0	6.300322566348764	0.0
23	0.0	0.0	0.0	6.551711801571236	0.0
24	0.0	0.0	0.0	6.732351092726212	0.0
25	0.0	0.0	0.0	6.8927532300686725	0.0
26	0.0	0.0	0.0	7.04594688254388	0.0
27	0.0	0.0	0.0	7.195776575414368	0.0
28	0.0	0.0	0.0	7.358047579203895	0.0
29	0.0	0.0	0.0	7.527153294571264	0.0
30	0.0	0.0	0.0	7.742767086378473	0.0
31	0.0	0.0	0.0	7.940226175557043	0.0
32	0.0	0.0	0.0	8.122307163685468	0.0
33	0.0	0.0	0.0	8.299315514314715	0.0
34	0.0	0.0	0.0	8.489085552968215	0.0
35	0.0	0.0	0.0	8.720344426746577	0.0
36	0.0	0.0	0.0	8.912410501320755	0.0
37	0.0	0.0	0.0	9.122204109049958	0.0
38	0.0	0.0	0.0	9.324575647175102	0.0
39	0.0	0.0	0.0	9.542538871087194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGA	60	0.0	45.000004	14
CGCATTA	30	2.1662363E-6	45.000004	44
GTAGCGT	30	2.1662363E-6	45.000004	38
CGCTCGA	30	2.1662363E-6	45.000004	19
CGTAGCG	30	2.1662363E-6	45.000004	4
ACGTACA	30	2.1662363E-6	45.000004	25
CCAACCG	30	2.1662363E-6	45.000004	25
ATACGTC	60	0.0	45.000004	26
AACCGTA	25	3.8917497E-5	45.0	12
TACCCGT	25	3.8917497E-5	45.0	44
CGACGTG	25	3.8917497E-5	45.0	40
GCGCGAC	35	1.2124838E-7	45.0	9
CGTCTAG	20	7.034425E-4	45.0	11
CGCGACA	35	1.2124838E-7	45.0	24
TAACGCG	25	3.8917497E-5	45.0	1
CTACGCT	20	7.034425E-4	45.0	31
AACGCTA	25	3.8917497E-5	45.0	43
ACTACGC	20	7.034425E-4	45.0	26
ACTAACG	40	6.8193913E-9	45.0	21
TCCCGAA	45	3.8562575E-10	45.0	36
>>END_MODULE