##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547464_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2098428
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230220908222726	31.0	31.0	33.0	30.0	34.0
2	31.627254783104306	31.0	31.0	34.0	30.0	34.0
3	31.76940738495674	31.0	31.0	34.0	30.0	34.0
4	35.511598205895076	37.0	35.0	37.0	33.0	37.0
5	35.365927256022125	37.0	35.0	37.0	33.0	37.0
6	35.42078498761931	37.0	35.0	37.0	33.0	37.0
7	35.8697124704779	37.0	35.0	37.0	35.0	37.0
8	35.9008638847747	37.0	35.0	37.0	35.0	37.0
9	37.61987449652788	39.0	37.0	39.0	35.0	39.0
10	36.94028482273397	39.0	37.0	39.0	32.0	39.0
11	36.7622067566769	39.0	35.0	39.0	32.0	39.0
12	36.2104785105803	38.0	35.0	39.0	32.0	39.0
13	35.98317311816274	38.0	35.0	39.0	31.0	39.0
14	37.06811622795731	39.0	35.0	41.0	31.0	41.0
15	37.23419721810803	39.0	35.0	41.0	32.0	41.0
16	37.30788142361806	39.0	35.0	41.0	32.0	41.0
17	37.22365694700986	39.0	35.0	41.0	32.0	41.0
18	37.148397752984614	39.0	35.0	41.0	32.0	41.0
19	37.09623823166675	39.0	35.0	41.0	32.0	41.0
20	36.970727611335725	39.0	35.0	41.0	31.0	41.0
21	36.90948605336948	39.0	35.0	41.0	31.0	41.0
22	36.81948534807961	38.0	35.0	41.0	31.0	41.0
23	36.74441963221993	38.0	35.0	40.0	31.0	41.0
24	36.64353411220208	38.0	35.0	40.0	31.0	41.0
25	36.5503505481246	38.0	35.0	40.0	31.0	41.0
26	36.412528330731384	38.0	35.0	40.0	30.0	41.0
27	36.304840099350564	38.0	35.0	40.0	30.0	41.0
28	36.221632574479564	38.0	35.0	40.0	30.0	41.0
29	36.26034298055497	38.0	35.0	40.0	30.0	41.0
30	36.2428927749725	38.0	35.0	40.0	30.0	41.0
31	36.134127070359334	38.0	35.0	40.0	30.0	41.0
32	36.043106077501825	38.0	35.0	40.0	30.0	41.0
33	35.93475925788257	38.0	35.0	40.0	30.0	41.0
34	35.80729002853565	38.0	35.0	40.0	29.0	41.0
35	35.70488336983685	38.0	35.0	40.0	29.0	41.0
36	35.622716147516144	38.0	35.0	40.0	27.0	41.0
37	35.543581671613225	38.0	34.0	40.0	27.0	41.0
38	35.45751486350735	38.0	34.0	40.0	27.0	41.0
39	35.37314980547343	38.0	34.0	40.0	27.0	41.0
40	35.22836904578094	38.0	34.0	40.0	26.0	41.0
41	35.15671588446208	38.0	34.0	40.0	26.0	41.0
42	35.09231148269085	38.0	34.0	40.0	26.0	41.0
43	35.038222898283855	38.0	34.0	40.0	26.0	41.0
44	34.89775060187912	38.0	34.0	40.0	25.0	41.0
45	34.789544840232786	37.0	34.0	40.0	24.0	41.0
46	34.74329831664465	37.0	34.0	40.0	24.0	41.0
47	34.69756503439718	37.0	33.0	40.0	24.0	41.0
48	34.63958210622428	37.0	34.0	40.0	24.0	41.0
49	34.57363702733665	37.0	33.0	40.0	24.0	41.0
50	34.428452155613634	37.0	33.0	40.0	24.0	41.0
51	33.86891473045537	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	6.0
14	22.0
15	38.0
16	140.0
17	407.0
18	989.0
19	1781.0
20	3208.0
21	5106.0
22	7712.0
23	11206.0
24	16349.0
25	23020.0
26	30201.0
27	36180.0
28	41429.0
29	47974.0
30	57091.0
31	67816.0
32	81794.0
33	103597.0
34	158356.0
35	227939.0
36	161172.0
37	198995.0
38	295110.0
39	520466.0
40	321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56261353737179	24.035039562949027	29.858922965191088	13.543423934488102
2	28.408551544298877	27.24839737174685	30.76064558803066	13.582405495923616
3	29.091681963831974	25.71115139523491	32.35874664272493	12.838419998208185
4	25.785159176297682	29.44742445297146	30.528519444079095	14.238896926651758
5	23.219619639082207	32.68427603901588	30.35071968159022	13.7453846403117
6	21.410884719418537	42.481943626371745	25.944707180803917	10.162464473405807
7	88.72556027654987	3.6881417899494293	6.0328016972705285	1.5534962362301685
8	88.28489707533448	3.5528976929396676	5.436736452239486	2.7254687794863584
9	82.39434471899918	7.421317290848196	7.570190637944213	2.6141473522084153
10	31.233761653961917	45.69911381281607	11.940033205809302	11.12709132741271
11	35.441578171850544	23.005602288951536	25.705194555162247	15.847624984035669
12	33.714523443263246	22.98601619879262	26.020001639322388	17.279458718621747
13	23.801912669865253	31.995331743571853	27.12020617338312	17.08254941317977
14	18.760043232362513	33.823605098673866	28.538887205088763	18.877464463874862
15	16.91575789114518	27.355143945848987	37.939543315281725	17.789554847724105
16	20.47818652820111	24.456497911770146	36.10955439023879	18.955761169789955
17	19.23706698538144	26.14981309818588	28.284649270787465	26.32847064564522
18	20.507446526638034	26.87592807568332	32.04889564950525	20.567729748173395
19	25.23284096476029	27.07874656647738	27.350664402114344	20.337748066647986
20	26.107924598794906	26.294921722355973	28.13687198226482	19.4602816965843
21	23.25388338318017	28.201968330578893	29.723440594578417	18.82070769166252
22	23.53104323808108	25.06233237452036	27.180632359080224	24.22599202831834
23	20.030518083060272	29.19342479227307	27.527034522985776	23.249022601680878
24	19.170159757685276	26.57570333602106	35.17556952156567	19.078567384727997
25	19.17435337309643	27.97165306600941	31.566486913060633	21.28750664783352
26	19.684115919154717	32.69209141319121	27.326980005985433	20.29681266166864
27	19.199943958048596	33.31417613565964	27.972463196259294	19.513416710032462
28	17.811666638073834	30.224434672049743	33.13475611267101	18.829142577205413
29	18.54478685949673	26.70179772667921	32.349263353329256	22.40415206049481
30	20.168621463304913	30.59890546637769	29.496127577405563	19.736345492911838
31	22.886989689424656	29.05837131414564	27.985044042492763	20.06959495393695
32	25.163312727432153	27.921996847163687	28.79488836405157	18.119802061352594
33	24.54403963347801	28.685711399199782	26.125795119012903	20.644453848309304
34	19.638462696837824	28.31376630506265	28.724168758708903	23.323602239390627
35	21.487847093157352	26.154530915523427	29.32552367772447	23.032098313594748
36	25.21721021640962	27.209701738634827	27.797189133961233	19.775898910994325
37	20.38373487200895	31.000015249510586	28.3294447081339	20.286805170346565
38	20.003021309284854	30.722760085168517	27.292239714681653	21.98197889086497
39	20.45059444498453	30.09605285480369	28.978597311892525	20.474755388319256
40	22.821083210860703	27.479904004330862	27.76406910315722	21.934943681651216
41	19.03729839670458	26.601007992649734	28.251767513586362	26.109926097059322
42	21.121048708843002	26.83494501598339	27.226714473882353	24.81729180129125
43	21.021736271151546	26.577800143726638	29.14495994144188	23.255503643679937
44	19.84333034061688	28.10074970406419	29.134857140678644	22.92106281464029
45	19.341669097057416	30.52165716431538	26.629886753322012	23.50678698530519
46	20.879153347172263	30.44250267342982	27.534754587719952	21.14358939167796
47	20.74414752376541	27.187304019961612	28.48680059549339	23.581747860779593
48	20.31530269325419	25.931649787364634	31.372627509735864	22.380420009645317
49	21.477029471585396	25.37442313960736	31.171000386956333	21.97754700185091
50	19.87049353134823	28.46974020552528	29.513616859858903	22.14614940326759
51	19.029768950852734	27.815250273061547	28.271782496230514	24.883198279855208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1609.0
1	2383.0
2	3157.0
3	10512.0
4	17867.0
5	12436.5
6	7006.0
7	7389.5
8	7773.0
9	8698.0
10	9623.0
11	10186.0
12	10749.0
13	11033.0
14	11317.0
15	11069.0
16	10821.0
17	10335.5
18	9850.0
19	9789.5
20	9729.0
21	10273.5
22	10818.0
23	11894.0
24	12970.0
25	16677.5
26	20700.0
27	21015.0
28	26171.0
29	31327.0
30	34996.0
31	38665.0
32	45971.5
33	53278.0
34	59626.0
35	65974.0
36	70414.0
37	74854.0
38	84051.5
39	93249.0
40	114251.0
41	135253.0
42	153774.0
43	172295.0
44	176646.5
45	180998.0
46	187633.5
47	194269.0
48	191174.0
49	188079.0
50	177128.5
51	166178.0
52	149588.0
53	132998.0
54	115643.5
55	98289.0
56	90762.0
57	83235.0
58	74997.5
59	66760.0
60	59276.5
61	51793.0
62	47241.0
63	42689.0
64	33850.5
65	25012.0
66	20548.0
67	16084.0
68	12804.0
69	9524.0
70	7426.5
71	5329.0
72	4266.5
73	3204.0
74	2607.0
75	1585.0
76	1160.0
77	947.5
78	735.0
79	539.0
80	343.0
81	214.0
82	85.0
83	53.5
84	22.0
85	26.0
86	30.0
87	21.5
88	13.0
89	7.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2098428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.867577315902274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40486753477327	14.00905032620637
2	9.20655726036247	3.289977473256156
3	3.301071069511542	1.7694642767935704
4	1.5999606538032933	1.1434968273692758
5	0.9086847201749269	0.8117997246752263
6	0.6156007300004103	0.6599576183404925
7	0.41976264509333966	0.5250099060873025
8	0.30444136918728987	0.4351703761690439
9	0.2185508103821706	0.3514476151760877
>10	2.1555692582859916	9.289211094656403
>50	1.1902177696103233	15.657475113092392
>100	1.6620371098071531	49.29096679080196
>500	0.009172092473770418	1.092280671038949
>1k	0.0032372091083895595	1.0894850186440743
>5k	0.0	0.0
>10k+	2.6976742569912994E-4	0.5852071676926826
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12141	0.578575962577701	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3552	0.1692695675048179	No Hit
GAGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	2705	0.12890601917244718	No Hit
AAGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	2219	0.10574582496992986	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2175	0.10364901726435218	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.530944116262269E-5	0.0	0.0	0.10698484770504396	0.0
2	9.530944116262269E-5	0.0	0.0	0.33811024252440397	0.0
3	9.530944116262269E-5	0.0	0.0	0.49970740001563074	0.0
4	9.530944116262269E-5	0.0	0.0	0.9685345410945717	0.0
5	9.530944116262269E-5	0.0	0.0	1.468766143036597	0.0
6	9.530944116262269E-5	0.0	0.0	2.132119853528451	0.0
7	9.530944116262269E-5	0.0	0.0	2.3922193184612484	0.0
8	9.530944116262269E-5	0.0	0.0	3.137300874750051	0.0
9	9.530944116262269E-5	0.0	0.0	3.351699462645371	0.0
10	9.530944116262269E-5	0.0	0.0	3.811567516255025	0.0
11	9.530944116262269E-5	0.0	0.0	4.412684161667687	0.0
12	9.530944116262269E-5	0.0	0.0	4.913344656094943	0.0
13	9.530944116262269E-5	0.0	0.0	5.1105398898604095	0.0
14	9.530944116262269E-5	0.0	0.0	5.196890243553746	0.0
15	9.530944116262269E-5	0.0	0.0	5.335470171004199	0.0
16	9.530944116262269E-5	0.0	0.0	5.631787223578793	0.0
17	9.530944116262269E-5	0.0	0.0	5.961843818324955	0.0
18	9.530944116262269E-5	0.0	0.0	6.39264249238001	0.0
19	9.530944116262269E-5	0.0	0.0	6.593507139630238	0.0
20	9.530944116262269E-5	0.0	0.0	6.816149994186124	0.0
21	9.530944116262269E-5	0.0	0.0	7.097980011703999	0.0
22	9.530944116262269E-5	0.0	0.0	7.392152601852434	0.0
23	9.530944116262269E-5	0.0	0.0	7.6808925538546	0.0
24	9.530944116262269E-5	0.0	0.0	7.916688111290928	0.0
25	9.530944116262269E-5	0.0	0.0	8.109022563557101	0.0
26	9.530944116262269E-5	0.0	0.0	8.29144483394236	0.0
27	9.530944116262269E-5	0.0	0.0	8.465241599902404	0.0
28	9.530944116262269E-5	0.0	0.0	8.661626703417987	0.0
29	9.530944116262269E-5	0.0	0.0	8.882077440827134	0.0
30	9.530944116262269E-5	0.0	0.0	9.139412931966215	0.0
31	1.4296416174393404E-4	0.0	0.0	9.372587479770571	0.0
32	1.4296416174393404E-4	0.0	0.0	9.58913053009205	0.0
33	1.4296416174393404E-4	0.0	0.0	9.800765144193653	0.0
34	1.4296416174393404E-4	0.0	0.0	10.02741099527837	0.0
35	1.4296416174393404E-4	0.0	0.0	10.278408408580137	0.0
36	1.4296416174393404E-4	0.0	0.0	10.52016080608913	0.0
37	1.4296416174393404E-4	0.0	0.0	10.754622031349182	0.0
38	1.4296416174393404E-4	0.0	0.0	10.99008400574144	0.0
39	1.4296416174393404E-4	0.0	0.0	11.23393321095601	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGGA	40	6.8193913E-9	45.000004	8
CATGCGT	30	2.1663618E-6	45.000004	30
CGCATCG	35	1.2125747E-7	45.000004	21
GTCACGA	20	7.0346193E-4	45.000004	41
CGTAAGA	20	7.0346193E-4	45.000004	34
CGTAAAC	20	7.0346193E-4	45.000004	38
ACCGTTA	40	6.8193913E-9	45.000004	35
ACGTATC	20	7.0346193E-4	45.000004	35
AACTCGC	20	7.0346193E-4	45.000004	16
CACCGTA	30	2.1663618E-6	45.000004	40
GCCCGTA	30	2.1663618E-6	45.000004	14
GCGTACA	20	7.0346193E-4	45.000004	12
CCGAATA	25	3.8919105E-5	45.0	41
CGACGGT	495	0.0	43.636364	28
TATAGCG	95	0.0	42.63158	1
CGGTCTA	520	0.0	42.403843	31
ATACGAA	365	0.0	41.917805	19
CATACGA	385	0.0	41.493507	18
CGTTTTT	6325	0.0	41.47826	1
CGATCAT	60	3.6379788E-12	41.250004	10
>>END_MODULE