FastQCFastQC Report
Thu 26 May 2016
SRR1547463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547463_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4345635
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318940.7339318649633483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT164050.042.2020721
CGACGGT6050.042.0247928
CGTTAGG6500.041.5384642
CGGTCTA6150.040.9756131
TCGCGCG403.4611912E-739.3751
AAGGGAT87050.038.796675
TAGGGAC61750.038.7327965
TAAGGGA79000.038.4778484
TAGGGAG84650.038.434735
GGGCGAT73850.038.2058227
TTAGGGA79850.038.1527864
GACCGAT24150.038.105599
TACGGGA24300.037.870374
ATAGGGA87700.037.8677334
CGAGGGA39300.037.557254
TAGGGAT93600.037.5480775
CTATACG601.5643309E-1037.4999961
AGGGCGA40750.037.4355856
ACGGGAT27300.037.417585
CACGACG6800.037.3897126
Produced by FastQC (version 0.11.5)