##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547463_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4345635
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.327001692502936	31.0	31.0	33.0	30.0	34.0
2	31.74440283180709	31.0	31.0	34.0	30.0	34.0
3	31.869898415306395	31.0	31.0	34.0	30.0	34.0
4	35.62710835125361	37.0	35.0	37.0	33.0	37.0
5	35.51278466783335	37.0	35.0	37.0	33.0	37.0
6	35.565866668507596	37.0	35.0	37.0	33.0	37.0
7	35.97313511143941	37.0	35.0	37.0	35.0	37.0
8	36.01057198775323	37.0	35.0	37.0	35.0	37.0
9	37.763874094349845	39.0	38.0	39.0	35.0	39.0
10	37.05961407251184	39.0	37.0	39.0	33.0	39.0
11	36.91240520660387	39.0	37.0	39.0	32.0	39.0
12	36.66250409894066	39.0	35.0	39.0	32.0	39.0
13	36.59185665616187	39.0	35.0	39.0	32.0	39.0
14	37.71534401761768	40.0	36.0	41.0	32.0	41.0
15	37.78675015273947	40.0	36.0	41.0	32.0	41.0
16	37.802844463467366	40.0	36.0	41.0	33.0	41.0
17	37.73591385378661	40.0	36.0	41.0	32.0	41.0
18	37.61259815884215	39.0	36.0	41.0	32.0	41.0
19	37.569972397589765	39.0	36.0	41.0	32.0	41.0
20	37.45947347165604	39.0	35.0	41.0	32.0	41.0
21	37.42590714590618	39.0	35.0	41.0	32.0	41.0
22	37.35345927580204	39.0	35.0	41.0	32.0	41.0
23	37.25503016245037	39.0	35.0	41.0	32.0	41.0
24	37.18827812275997	39.0	35.0	41.0	32.0	41.0
25	37.08496917021333	39.0	35.0	41.0	31.0	41.0
26	36.97560793762016	39.0	35.0	41.0	31.0	41.0
27	36.88517719504745	39.0	35.0	41.0	31.0	41.0
28	36.76896080779909	39.0	35.0	40.0	31.0	41.0
29	36.83308814477056	39.0	35.0	41.0	31.0	41.0
30	36.78368615863965	39.0	35.0	41.0	31.0	41.0
31	36.71504302593292	39.0	35.0	41.0	30.0	41.0
32	36.66063210554959	39.0	35.0	41.0	30.0	41.0
33	36.561014443228665	39.0	35.0	41.0	30.0	41.0
34	36.40973275482179	39.0	35.0	41.0	30.0	41.0
35	36.298715607730514	39.0	35.0	41.0	30.0	41.0
36	36.262464749110315	39.0	35.0	41.0	30.0	41.0
37	36.22954735038723	39.0	35.0	41.0	29.0	41.0
38	36.12703298827444	39.0	35.0	41.0	29.0	41.0
39	36.053854499975266	39.0	35.0	41.0	29.0	41.0
40	35.98064701706425	39.0	35.0	41.0	28.0	41.0
41	35.88394630474028	39.0	35.0	41.0	28.0	41.0
42	35.847806822248074	39.0	35.0	41.0	28.0	41.0
43	35.84165145024835	39.0	35.0	41.0	28.0	41.0
44	35.72631203495001	38.0	35.0	41.0	27.0	41.0
45	35.60941910675885	38.0	35.0	40.0	27.0	41.0
46	35.58132631939866	38.0	34.0	40.0	27.0	41.0
47	35.534091105212475	38.0	34.0	40.0	27.0	41.0
48	35.45029644689441	38.0	34.0	40.0	27.0	41.0
49	35.37238723454685	38.0	34.0	40.0	27.0	41.0
50	35.295913945833	38.0	34.0	40.0	26.0	41.0
51	34.74783524156999	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	4.0
13	6.0
14	17.0
15	77.0
16	182.0
17	535.0
18	1230.0
19	2391.0
20	4177.0
21	7246.0
22	11065.0
23	17117.0
24	26056.0
25	39439.0
26	54259.0
27	64940.0
28	72484.0
29	82836.0
30	99480.0
31	122149.0
32	149458.0
33	190286.0
34	271609.0
35	349003.0
36	351703.0
37	456316.0
38	694061.0
39	1276839.0
40	665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.34783869330949	24.980284814532283	28.731290133662856	13.940586358495363
2	30.74864778104926	26.074923457676498	30.2533001506109	12.923128610663342
3	27.89569303450474	25.90776721929016	32.78068682712653	13.415852919078569
4	24.870887683848274	28.5823360682616	32.12319948638116	14.423576761508963
5	22.419462288020046	32.54233270856848	30.898637368301756	14.139567635109715
6	21.526773417463733	41.44623282903419	26.8940442536016	10.132949499900475
7	90.35708245170153	2.2720269880006025	6.169040888155585	1.2018496721422762
8	90.75108701029883	1.9621988501105132	5.771055323330192	1.5156588162604545
9	84.79975883846664	5.616371370352089	7.332829379365731	2.2510404118155343
10	27.083360659604406	44.362814640438046	14.83021468669136	13.723610013266185
11	27.903378907800587	25.25313331653487	28.90380807407893	17.939679701585614
12	28.620466283983813	22.897021954213827	29.04321232685212	19.439299434950243
13	24.853536939940884	24.20493207551946	31.010726855798982	19.93080412874068
14	20.36639064256432	27.670432514465666	31.152225163871332	20.810951679098682
15	19.368745879485967	27.356991555894595	33.97956800329526	19.29469456132418
16	23.694903046390227	26.25353947121652	31.995255008761664	18.056302473631586
17	22.900174542960926	26.36401814694515	30.00951529523303	20.72629201486089
18	22.707659525017633	25.89867763859597	32.031705377925206	19.36195745846119
19	23.78842217535527	27.085040506163082	29.335137442514153	19.791399875967493
20	24.566030050844123	27.351905993025184	29.69977920372972	18.382284752400974
21	24.55622711065241	25.93464476422893	31.086043811778946	18.423084313339707
22	23.872253422112074	23.70355540674723	31.482717715592774	20.941473455547925
23	21.55519273938101	26.92669770931061	31.063446423825287	20.454663127483094
24	20.800251286635902	26.444029468650726	32.65531964833678	20.100399596376594
25	21.27716662812224	28.146956658808207	30.361960910200697	20.213915802868858
26	21.09162412397728	29.474725788060894	28.960600694720107	20.473049393241723
27	20.176637016224326	28.802395967447797	30.462383518173986	20.558583498153897
28	19.261097630150715	28.442310502377673	32.03927619323758	20.25731567423403
29	20.478963373592123	26.479720455123356	31.490495635275394	21.550820536009123
30	21.155274200433308	27.269984708794	31.170197220889467	20.404543869883227
31	22.278079037931164	27.265359377858474	30.024357775100764	20.432203809109602
32	23.013230517519304	27.55983878075356	30.00327915252892	19.42365154919822
33	23.06484552890429	27.55567368175192	29.19041751090462	20.18906327843917
34	20.956707132559455	27.5675706772428	30.56002172294728	20.91570046725047
35	21.81161096134397	27.8493937019561	29.795553469170788	20.54344186752914
36	22.80014313213144	28.299891730437555	28.94318091602263	19.956784221408377
37	21.390682834614505	29.634219164748078	29.2483607113805	19.72673728925692
38	21.447291362482122	29.11047522398913	28.97229058584073	20.469942827688016
39	21.335132840194817	28.78375657412553	28.774137726707373	21.106972858972277
40	22.42468592046962	27.577005431887397	29.775878553997288	20.222430093645695
41	20.052213312899035	27.967650297367356	29.989104008965317	21.99103238076829
42	20.85964421770351	28.47194943891974	29.30724738732084	21.361158956055903
43	21.30703568063125	27.77251195740093	29.55492120254002	21.365531159427796
44	21.32236140402956	27.963600256349185	29.3999150872082	21.314123252413054
45	20.745644767680673	28.17878169703622	28.77494313259167	22.30063040269144
46	21.065344880552555	28.74558033520993	29.153760037370834	21.035314746866685
47	21.14266384544491	28.06404587591917	29.91509871399692	20.878191564639
48	20.94377461521734	27.836783346967703	30.462498576157454	20.7569434616575
49	21.246054949391745	27.760499903926583	30.014370742135497	20.97907440454617
50	20.449462506630216	27.9127676392518	30.410078158888172	21.22769169522981
51	20.47153062785991	27.78162454969182	29.821970782175676	21.924874040272595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1662.0
1	3126.5
2	4591.0
3	27432.5
4	50274.0
5	34468.0
6	18662.0
7	18371.0
8	18080.0
9	18634.5
10	19189.0
11	19543.5
12	19898.0
13	19656.5
14	19415.0
15	18994.0
16	18573.0
17	17963.5
18	17354.0
19	17423.5
20	17493.0
21	20467.5
22	23442.0
23	24997.0
24	26552.0
25	32838.0
26	47537.0
27	55950.0
28	67051.0
29	78152.0
30	91115.5
31	104079.0
32	119884.0
33	135689.0
34	151723.0
35	167757.0
36	178360.0
37	188963.0
38	205205.0
39	221447.0
40	240210.5
41	258974.0
42	276108.5
43	293243.0
44	303781.0
45	314319.0
46	329039.5
47	343760.0
48	352191.5
49	360623.0
50	342719.0
51	324815.0
52	297768.0
53	270721.0
54	250967.5
55	231214.0
56	214681.0
57	198148.0
58	180586.0
59	163024.0
60	140708.0
61	118392.0
62	101900.0
63	85408.0
64	71320.5
65	57233.0
66	44822.0
67	32411.0
68	26177.0
69	19943.0
70	15934.5
71	11926.0
72	9414.0
73	6902.0
74	5439.0
75	3090.0
76	2204.0
77	1685.0
78	1166.0
79	826.0
80	486.0
81	349.5
82	213.0
83	140.0
84	67.0
85	73.0
86	79.0
87	53.0
88	27.0
89	16.5
90	6.0
91	5.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4345635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.158403406055136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56805911711923	13.90353705467329
2	8.587192782700452	3.1185942134767997
3	2.7768152856254655	1.5126759642146226
4	1.4696397676732265	1.067452470519664
5	0.9605392900732743	0.8720929958258165
6	0.6397761301655416	0.6970387836666447
7	0.4809250855214658	0.611298219769324
8	0.3855341107065082	0.5600547127202798
9	0.3309130305189307	0.540796707043468
>10	4.61137566248507	21.173077091146546
>50	2.0810184091900257	27.21827434360139
>100	1.1032065071180956	27.200637768666976
>500	0.0035932048942287466	0.4333556891508257
>1k	0.0012832874622243698	0.34790559977529256
>5k	0.0	0.0
>10k+	1.28328746222437E-4	0.7432083857490409
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31894	0.7339318649633483	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04873856179821821	0.0
2	0.0	0.0	0.0	0.13880595125913706	0.0
3	0.0	0.0	0.0	0.21791982069363855	0.0
4	0.0	0.0	0.0	0.3564036095990574	0.0
5	0.0	0.0	0.0	0.5944355658033866	0.0
6	0.0	0.0	0.0	0.9628052056834041	0.0
7	0.0	0.0	0.0	1.1514082521886906	0.0
8	0.0	0.0	0.0	1.6435802822832566	0.0
9	0.0	0.0	0.0	1.843090825621572	0.0
10	0.0	0.0	0.0	2.197653507485097	0.0
11	0.0	0.0	0.0	2.5599250742411637	0.0
12	0.0	0.0	0.0	2.870811745579185	0.0
13	0.0	0.0	0.0	3.0151634916416126	0.0
14	0.0	0.0	0.0	3.0877190560182806	0.0
15	0.0	0.0	0.0	3.177165132368457	0.0
16	0.0	0.0	0.0	3.346576507230819	0.0
17	0.0	0.0	0.0	3.5626784117856194	0.0
18	0.0	0.0	0.0	3.8065553135502634	0.0
19	0.0	0.0	0.0	3.95067694364575	0.0
20	0.0	0.0	0.0	4.115808161523	0.0
21	0.0	0.0	0.0	4.331587903724082	0.0
22	0.0	0.0	0.0	4.570931520939978	0.0
23	0.0	0.0	0.0	4.819847962380642	0.0
24	0.0	0.0	0.0	5.014203908059467	0.0
25	0.0	0.0	0.0	5.186583778895375	0.0
26	0.0	0.0	0.0	5.349759011053621	0.0
27	0.0	0.0	0.0	5.521057336844903	0.0
28	0.0	0.0	0.0	5.706898991746891	0.0
29	2.3011596694154018E-5	0.0	0.0	5.903118876757942	0.0
30	2.3011596694154018E-5	0.0	0.0	6.1467196393622565	0.0
31	2.3011596694154018E-5	0.0	0.0	6.356263238859223	0.0
32	2.3011596694154018E-5	0.0	0.0	6.560284055149593	0.0
33	2.3011596694154018E-5	0.0	0.0	6.7634534423622785	0.0
34	2.3011596694154018E-5	0.0	0.0	6.9758965030427085	0.0
35	2.3011596694154018E-5	0.0	0.0	7.210154557389196	0.0
36	2.3011596694154018E-5	0.0	0.0	7.432538627841501	0.0
37	2.3011596694154018E-5	0.0	0.0	7.661342933771474	0.0
38	6.903479008246205E-5	0.0	0.0	7.905012731165871	0.0
39	6.903479008246205E-5	0.0	0.0	8.247172162411248	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	16405	0.0	42.202072	1
CGACGGT	605	0.0	42.02479	28
CGTTAGG	650	0.0	41.538464	2
CGGTCTA	615	0.0	40.97561	31
TCGCGCG	40	3.4611912E-7	39.375	1
AAGGGAT	8705	0.0	38.79667	5
TAGGGAC	6175	0.0	38.732796	5
TAAGGGA	7900	0.0	38.477848	4
TAGGGAG	8465	0.0	38.43473	5
GGGCGAT	7385	0.0	38.205822	7
TTAGGGA	7985	0.0	38.152786	4
GACCGAT	2415	0.0	38.10559	9
TACGGGA	2430	0.0	37.87037	4
ATAGGGA	8770	0.0	37.867733	4
CGAGGGA	3930	0.0	37.55725	4
TAGGGAT	9360	0.0	37.548077	5
CTATACG	60	1.5643309E-10	37.499996	1
AGGGCGA	4075	0.0	37.435585	6
ACGGGAT	2730	0.0	37.41758	5
CACGACG	680	0.0	37.38971	26
>>END_MODULE