FastQCFastQC Report
Thu 26 May 2016
SRR1547462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547462_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2260194
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116480.5153539917369925No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27220.12043213989595583No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.034736E-445.038
AACGCGT253.8920065E-545.015
CGAATTA351.2126293E-745.040
CGAATCG253.8920065E-545.019
CGTACTC207.034736E-445.038
CCGGTCG207.034736E-445.018
CGACTTA253.8920065E-545.028
ACGGGAT16550.042.416925
CGGTCTA6050.041.65289331
TATAGCG603.6379788E-1241.2499961
CGTAAGG4050.041.111112
CGTTTTT61900.041.1106641
TAGTGCG1700.041.0294151
CGCGAGG1650.040.909092
CGACGGT6200.040.6451628
ACGTCGA501.0822987E-940.510
TAATGCG501.0822987E-940.51
CGTATGG1450.040.3448262
TACGGGA16200.040.2777754
CGACGTA451.9297659E-840.044