##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547462_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2260194
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22664116443102	31.0	31.0	33.0	30.0	34.0
2	31.63634316346296	31.0	31.0	34.0	30.0	34.0
3	31.801231221744683	31.0	31.0	34.0	30.0	34.0
4	35.54012487423646	37.0	35.0	37.0	33.0	37.0
5	35.39863746209396	37.0	35.0	37.0	33.0	37.0
6	35.439933032297226	37.0	35.0	37.0	33.0	37.0
7	35.8807996127766	37.0	35.0	37.0	35.0	37.0
8	35.90482100209097	37.0	35.0	37.0	35.0	37.0
9	37.620347633875674	39.0	37.0	39.0	35.0	39.0
10	36.95094934328646	39.0	37.0	39.0	32.0	39.0
11	36.798403145924645	39.0	37.0	39.0	32.0	39.0
12	36.3575865611536	38.0	35.0	39.0	32.0	39.0
13	36.184582385405854	38.0	35.0	39.0	32.0	39.0
14	37.31558839639429	39.0	36.0	41.0	32.0	41.0
15	37.43928972468735	39.0	36.0	41.0	32.0	41.0
16	37.487099779930396	39.0	36.0	41.0	32.0	41.0
17	37.40492895742578	39.0	35.0	41.0	32.0	41.0
18	37.30790940954626	39.0	36.0	41.0	32.0	41.0
19	37.25814819435854	39.0	36.0	41.0	32.0	41.0
20	37.151713083036235	39.0	35.0	41.0	32.0	41.0
21	37.10283630520212	39.0	35.0	41.0	31.0	41.0
22	37.01245468309357	39.0	35.0	41.0	31.0	41.0
23	36.90927106257251	39.0	35.0	41.0	31.0	41.0
24	36.82957259421094	39.0	35.0	41.0	31.0	41.0
25	36.723790524176245	38.0	35.0	40.0	31.0	41.0
26	36.5869690831849	38.0	35.0	40.0	31.0	41.0
27	36.47927832743561	38.0	35.0	40.0	30.0	41.0
28	36.3810854289499	38.0	35.0	40.0	30.0	41.0
29	36.44408754292773	38.0	35.0	40.0	30.0	41.0
30	36.40254995810094	38.0	35.0	40.0	30.0	41.0
31	36.31321824586739	38.0	35.0	40.0	30.0	41.0
32	36.25774955601156	38.0	35.0	40.0	30.0	41.0
33	36.183990400824	38.0	35.0	41.0	30.0	41.0
34	36.09511041972503	38.0	35.0	41.0	30.0	41.0
35	35.995311464414115	38.0	35.0	41.0	29.0	41.0
36	35.95045248328241	38.0	35.0	41.0	29.0	41.0
37	35.881380536361036	38.0	35.0	41.0	29.0	41.0
38	35.79698114409648	38.0	35.0	41.0	28.0	41.0
39	35.71725480202142	38.0	35.0	40.0	27.0	41.0
40	35.58029797442166	38.0	34.0	40.0	27.0	41.0
41	35.478818189942984	38.0	34.0	40.0	27.0	41.0
42	35.42932332357311	38.0	34.0	40.0	27.0	41.0
43	35.39360116874923	38.0	34.0	40.0	27.0	41.0
44	35.261995209260796	38.0	34.0	40.0	26.0	41.0
45	35.14795632587291	38.0	34.0	40.0	26.0	41.0
46	35.07997853281621	38.0	34.0	40.0	26.0	41.0
47	35.038150264977254	38.0	34.0	40.0	26.0	41.0
48	34.972272291670535	38.0	34.0	40.0	26.0	41.0
49	34.89233446332483	37.0	34.0	40.0	26.0	41.0
50	34.79238286624953	37.0	34.0	40.0	25.0	41.0
51	34.19628049627598	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	0.0
12	1.0
13	6.0
14	17.0
15	36.0
16	143.0
17	356.0
18	863.0
19	1682.0
20	2921.0
21	4705.0
22	7207.0
23	10797.0
24	16006.0
25	22602.0
26	29712.0
27	36254.0
28	42092.0
29	49574.0
30	59122.0
31	70548.0
32	85613.0
33	106614.0
34	157784.0
35	217405.0
36	180057.0
37	226710.0
38	336976.0
39	594052.0
40	336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.57370561996006	25.183944387074735	30.282135073360962	12.960214919604246
2	27.162004677474588	27.823850519026244	31.089278177006047	13.924866626493124
3	28.821508242212836	26.493079797574897	31.114629983089948	13.570781977122317
4	25.6581072244241	29.634004868608628	30.041492013517423	14.666395893449854
5	23.1692500732238	33.3026722484884	29.036091592137662	14.491986086150128
6	21.17888995369424	43.11961716560614	25.443125678592192	10.25836720210743
7	89.78472644383623	3.343208591828843	5.482670956563906	1.3893940077710145
8	88.92139347330362	3.416520882720687	5.114295498528002	2.5477901454476917
9	82.73515459292432	7.641556432766391	7.082046939333527	2.5412420349757587
10	28.066263338456785	49.47438140265836	11.5446284699455	10.914726788939356
11	34.26754517532566	23.916398326869288	25.431887705214685	16.38416879259037
12	32.41898704270518	23.255039169204057	25.78004365996901	18.545930128121746
13	24.120540095230762	30.20453111547062	27.12032683920053	18.55460195009809
14	19.230296160418085	32.54539212120729	28.324736726139438	19.89957499223518
15	17.61977069225031	28.899510396010253	35.10844644309294	18.372272468646496
16	21.31825852117119	26.039490415424517	34.13892789733979	18.503323166064504
17	20.54708578113206	26.76265842666603	27.79637500143793	24.89388079076398
18	21.491031300852935	27.000071675263275	30.929645862257843	20.57925116162595
19	24.661821064917437	27.683464339786763	26.933042030905312	20.721672564390488
20	26.160763191124303	26.846633519069602	27.973129740190444	19.019473549615654
21	23.928653912009324	28.103782241701374	28.786643978348764	19.180919867940542
22	24.565767363332526	25.239514838106818	27.332211305755173	22.862506492805483
23	20.65601448371246	29.091883263118124	27.833274488827065	22.418827764342353
24	20.318123134562786	26.977816948456635	32.93606655003951	19.767993366941067
25	20.216538934268474	29.500343775799777	29.063699841695005	21.219417448236744
26	20.525538958160226	32.074193631166175	26.401671714905888	20.99859569576771
27	20.147828018302853	31.439292379326726	28.108914544503698	20.303965057866712
28	18.64242626960341	30.04445636082566	31.367971067970274	19.94514630160066
29	19.82113039854101	27.069136543146293	30.266428457026258	22.843304601286437
30	20.579383893594976	29.908981264440133	29.03206538907722	20.479569452887674
31	23.333262542949853	29.183114369828434	26.814291162617014	20.669331924604702
32	23.71592880965085	29.344560688153322	27.40193983348332	19.537570668712508
33	22.999662860798676	29.749658657619655	26.217174277960208	21.033504203621458
34	20.45718199411201	29.22129693291815	27.49290547625558	22.828615596714265
35	20.467800551634063	27.707356094211384	28.46937032838774	23.355473025766816
36	23.68929392786637	29.029985921562485	26.92335259716644	20.357367553404707
37	19.833607203629423	31.94827523655049	27.381808818181092	20.83630874163899
38	19.215474423876888	31.28288987582482	26.871719861215453	22.62991583908284
39	20.422760170144684	29.528792661160946	28.042283096052817	22.00616407264155
40	22.121375421755832	28.45835357495861	27.47556183230289	21.94470917098267
41	18.742638906217785	28.136213086133317	28.80040385913776	24.320744148511146
42	21.06987276313449	27.9548127284649	27.45299739756853	23.522317110832077
43	21.156635226887605	28.11329469948155	28.349601848336913	22.380468225293935
44	20.459084485668043	28.84509028870973	27.90897595516137	22.786849270460856
45	19.94213771030274	30.272578371591113	26.608512366637555	23.17677155146859
46	20.86577523876269	29.921590801497572	27.037988774414938	22.1746451853248
47	20.43966137420062	28.30349961109533	28.338231142990377	22.918607871713668
48	20.520672119295956	26.854464705242115	30.309168151052518	22.31569502440941
49	20.815868018409038	26.86517175074352	29.612015605740037	22.706944625107404
50	19.864091312515654	28.691519400547033	28.768282722633543	22.676106564303772
51	18.487528061750453	28.732002651099865	28.4314089852464	24.34906030190329
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1521.0
1	2406.0
2	3291.0
3	10627.5
4	17964.0
5	12601.0
6	7238.0
7	7540.0
8	7842.0
9	8757.0
10	9672.0
11	10169.5
12	10667.0
13	10840.5
14	11014.0
15	11360.5
16	11707.0
17	11159.0
18	10611.0
19	10650.0
20	10689.0
21	10322.0
22	9955.0
23	11738.5
24	13522.0
25	17080.5
26	23798.0
27	26957.0
28	31033.0
29	35109.0
30	41238.5
31	47368.0
32	50706.5
33	54045.0
34	65249.0
35	76453.0
36	82900.0
37	89347.0
38	99199.0
39	109051.0
40	122942.0
41	136833.0
42	152570.0
43	168307.0
44	175448.5
45	182590.0
46	190985.0
47	199380.0
48	200954.5
49	202529.0
50	190054.5
51	177580.0
52	158420.5
53	139261.0
54	126413.0
55	113565.0
56	105402.5
57	97240.0
58	88466.5
59	79693.0
60	70683.0
61	61673.0
62	53290.5
63	44908.0
64	37619.5
65	30331.0
66	23540.5
67	16750.0
68	13024.5
69	9299.0
70	7924.0
71	6549.0
72	5265.0
73	3981.0
74	3230.5
75	1977.0
76	1474.0
77	1001.0
78	528.0
79	377.0
80	226.0
81	270.0
82	314.0
83	167.5
84	21.0
85	13.5
86	6.0
87	8.5
88	11.0
89	6.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2260194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.991670277395663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20744326159705	14.070825324007833
2	8.981984319179444	3.232018006148295
3	3.1904079021806946	1.7220230107929797
4	1.5246591656849615	1.0972465997765195
5	0.8772181437012787	0.7891309801411247
6	0.5505295997947012	0.5942968222471713
7	0.39100435740678957	0.492437503284155
8	0.2997939394002398	0.43150349670805205
9	0.23396069929076374	0.3788409383557505
>10	2.700136567863547	12.06085308063143
>50	1.4591839535387552	19.262795314904185
>100	1.5742336824258716	43.75345557262223
>500	0.006461963324592735	0.7693829234418673
>1k	0.002733907560404619	0.8243388465448578
>5k	0.0	0.0
>10k+	2.4853705094587443E-4	0.5208515803935179
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11648	0.5153539917369925	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2722	0.12043213989595583	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.848797935044514E-5	0.0	0.0	0.09322208624569395	0.0
2	8.848797935044514E-5	0.0	0.0	0.30577021264546317	0.0
3	8.848797935044514E-5	0.0	0.0	0.44969591105896217	0.0
4	8.848797935044514E-5	0.0	0.0	0.8101074509533253	0.0
5	8.848797935044514E-5	0.0	0.0	1.237637123185001	0.0
6	8.848797935044514E-5	0.0	0.0	1.7573712698998405	0.0
7	8.848797935044514E-5	0.0	0.0	1.98390049703698	0.0
8	8.848797935044514E-5	0.0	0.0	2.667779845446895	0.0
9	8.848797935044514E-5	0.0	0.0	2.8720543457773977	0.0
10	8.848797935044514E-5	0.0	0.0	3.28193066612866	0.0
11	8.848797935044514E-5	0.0	0.0	3.7746759791416133	0.0
12	8.848797935044514E-5	0.0	0.0	4.201011063652058	0.0
13	8.848797935044514E-5	0.0	0.0	4.375597846910487	0.0
14	8.848797935044514E-5	0.0	0.0	4.4472288661946715	0.0
15	8.848797935044514E-5	0.0	0.0	4.566864614276474	0.0
16	8.848797935044514E-5	0.0	0.0	4.81870140350784	0.0
17	8.848797935044514E-5	0.0	0.0	5.114207010548652	0.0
18	8.848797935044514E-5	0.0	0.0	5.477273189823529	0.0
19	8.848797935044514E-5	0.0	0.0	5.6653543899329	0.0
20	8.848797935044514E-5	0.0	0.0	5.880999595609935	0.0
21	8.848797935044514E-5	0.0	0.0	6.147702365372176	0.0
22	8.848797935044514E-5	0.0	0.0	6.426395256336403	0.0
23	8.848797935044514E-5	0.0	0.0	6.705619075176733	0.0
24	8.848797935044514E-5	0.0	0.0	6.919184813339032	0.0
25	8.848797935044514E-5	0.0	0.0	7.10071790297647	0.0
26	8.848797935044514E-5	0.0	0.0	7.279109669346967	0.0
27	8.848797935044514E-5	0.0	0.0	7.45347523265702	0.0
28	8.848797935044514E-5	0.0	0.0	7.655449045524411	0.0
29	8.848797935044514E-5	0.0	0.0	7.866315900316521	0.0
30	8.848797935044514E-5	0.0	0.0	8.120099425093597	0.0
31	8.848797935044514E-5	0.0	0.0	8.3588399933811	0.0
32	1.327319690256677E-4	0.0	0.0	8.578113206211503	0.0
33	1.327319690256677E-4	0.0	0.0	8.793714167898862	0.0
34	1.327319690256677E-4	0.0	0.0	9.028782485043319	0.0
35	1.327319690256677E-4	0.0	0.0	9.273761455875027	0.0
36	1.327319690256677E-4	0.0	0.0	9.527279516714051	0.0
37	1.327319690256677E-4	0.0	0.0	9.770665703917452	0.0
38	1.327319690256677E-4	0.0	0.0	10.01471555096598	0.0
39	1.327319690256677E-4	0.0	0.0	10.262703113095602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.034736E-4	45.0	38
AACGCGT	25	3.8920065E-5	45.0	15
CGAATTA	35	1.2126293E-7	45.0	40
CGAATCG	25	3.8920065E-5	45.0	19
CGTACTC	20	7.034736E-4	45.0	38
CCGGTCG	20	7.034736E-4	45.0	18
CGACTTA	25	3.8920065E-5	45.0	28
ACGGGAT	1655	0.0	42.41692	5
CGGTCTA	605	0.0	41.652893	31
TATAGCG	60	3.6379788E-12	41.249996	1
CGTAAGG	405	0.0	41.11111	2
CGTTTTT	6190	0.0	41.110664	1
TAGTGCG	170	0.0	41.029415	1
CGCGAGG	165	0.0	40.90909	2
CGACGGT	620	0.0	40.64516	28
ACGTCGA	50	1.0822987E-9	40.5	10
TAATGCG	50	1.0822987E-9	40.5	1
CGTATGG	145	0.0	40.344826	2
TACGGGA	1620	0.0	40.277775	4
CGACGTA	45	1.9297659E-8	40.0	44
>>END_MODULE