FastQCFastQC Report
Thu 26 May 2016
SRR1547461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547461_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4295499
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239430.5573974059824016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT128950.040.323771
TACGGGA27150.038.950274
CGACGGT8800.038.60795628
GGGCGAT71150.038.327487
ATAGGGA95400.037.7358474
TAGGGAC62850.037.6968965
CGTTAGG6450.037.674422
TAAGGGA80950.037.467574
TTAGGGA92950.037.3507274
TAGGGAT95200.037.1533625
AGGGCGA36800.037.0516326
ACGGGAC23200.037.0474135
TAGGGAG96300.036.985985
CGTAAGG6850.036.7883222
AAGGGAT90850.036.7776575
AGGGATC72400.036.4537286
GACCGAT27900.036.4516149
TATGGGA76100.036.3074874
AGGGATG110150.036.1348156
GGACCGA32100.036.098138
Produced by FastQC (version 0.11.5)