##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547461_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4295499
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26512193344708	31.0	31.0	33.0	30.0	34.0
2	31.685844182480313	31.0	31.0	34.0	30.0	34.0
3	31.82490718773302	31.0	31.0	34.0	30.0	34.0
4	35.568169611958936	37.0	35.0	37.0	33.0	37.0
5	35.44013512749043	37.0	35.0	37.0	33.0	37.0
6	35.480856822455316	37.0	35.0	37.0	33.0	37.0
7	35.91740098181841	37.0	35.0	37.0	35.0	37.0
8	35.95321405033501	37.0	35.0	37.0	35.0	37.0
9	37.65757435864844	39.0	37.0	39.0	35.0	39.0
10	36.988040504723664	39.0	37.0	39.0	33.0	39.0
11	36.85057079515092	39.0	37.0	39.0	32.0	39.0
12	36.53098836712568	39.0	35.0	39.0	32.0	39.0
13	36.44849178174643	39.0	35.0	39.0	32.0	39.0
14	37.509765454490854	40.0	36.0	41.0	32.0	41.0
15	37.61249344953869	40.0	36.0	41.0	32.0	41.0
16	37.633734520715755	40.0	36.0	41.0	32.0	41.0
17	37.56039426385619	39.0	36.0	41.0	32.0	41.0
18	37.45149492526945	39.0	36.0	41.0	32.0	41.0
19	37.41219262302238	39.0	36.0	41.0	32.0	41.0
20	37.310752487662086	39.0	35.0	41.0	32.0	41.0
21	37.25214928463492	39.0	35.0	41.0	32.0	41.0
22	37.17821794394551	39.0	35.0	41.0	31.0	41.0
23	37.077467367586394	39.0	35.0	41.0	31.0	41.0
24	37.01084996178558	39.0	35.0	41.0	31.0	41.0
25	36.89926478856123	39.0	35.0	41.0	31.0	41.0
26	36.760940463494464	39.0	35.0	40.0	31.0	41.0
27	36.660151707636295	38.0	35.0	40.0	30.0	41.0
28	36.53497183912742	38.0	35.0	40.0	30.0	41.0
29	36.59311013691308	38.0	35.0	40.0	30.0	41.0
30	36.537798984471884	38.0	35.0	40.0	30.0	41.0
31	36.445889522963455	38.0	35.0	40.0	30.0	41.0
32	36.43067569099655	39.0	35.0	41.0	30.0	41.0
33	36.34215815205637	39.0	35.0	41.0	30.0	41.0
34	36.21451035141668	39.0	35.0	41.0	30.0	41.0
35	36.11196394179116	39.0	35.0	41.0	29.0	41.0
36	36.061363068644646	39.0	35.0	41.0	29.0	41.0
37	36.03062531268195	39.0	35.0	41.0	29.0	41.0
38	35.921067843340204	38.0	35.0	41.0	28.0	41.0
39	35.83156601829031	38.0	35.0	41.0	28.0	41.0
40	35.756580085340495	38.0	35.0	40.0	27.0	41.0
41	35.65427346159317	38.0	35.0	40.0	27.0	41.0
42	35.60698675520586	38.0	34.0	40.0	27.0	41.0
43	35.58437331727932	38.0	34.0	40.0	27.0	41.0
44	35.46253625015394	38.0	34.0	40.0	27.0	41.0
45	35.332370930595026	38.0	34.0	40.0	26.0	41.0
46	35.28742062330826	38.0	34.0	40.0	26.0	41.0
47	35.23260277793104	38.0	34.0	40.0	26.0	41.0
48	35.149517902343824	38.0	34.0	40.0	26.0	41.0
49	35.07084997575369	38.0	34.0	40.0	26.0	41.0
50	34.97462832606875	38.0	34.0	40.0	26.0	41.0
51	34.41408250822547	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	3.0
11	8.0
12	6.0
13	6.0
14	31.0
15	91.0
16	255.0
17	651.0
18	1446.0
19	2847.0
20	5007.0
21	8225.0
22	12599.0
23	18935.0
24	28242.0
25	40517.0
26	55087.0
27	66846.0
28	76893.0
29	89382.0
30	107895.0
31	130836.0
32	158993.0
33	199008.0
34	277145.0
35	353841.0
36	357213.0
37	459086.0
38	680475.0
39	1163399.0
40	524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.873596059503218	24.89717725461	29.46619240279185	13.763034283094932
2	30.18666748612909	26.58191749084332	29.56874160603925	13.66267341698834
3	28.836323789156975	25.90593083597505	31.408551136899344	13.849194237968627
4	25.774816848985417	28.553469573616475	30.574398923151886	15.097314654246224
5	23.427243260911016	32.56809045933895	29.32879276656798	14.675873513182053
6	22.355190863738997	41.27990717725694	25.85464459426018	10.510257364743888
7	90.33788623859533	2.6650221545855324	5.58684800066302	1.4102436061561183
8	90.2680456915483	2.289349852019521	5.43152262403041	2.011081832401777
9	83.30359290038247	6.717985500636829	7.356747143929029	2.6216744550516715
10	26.052409743315035	50.50330590229447	12.318522248521068	11.125762105869423
11	31.153563299630616	23.992649049621477	27.648149842428086	17.205637808319825
12	29.53633559220943	22.127487400183306	28.332494082759652	20.003682924847613
13	25.952398079943677	24.538825407711652	30.060325936520993	19.44845057582367
14	20.855015913168646	27.85657731499879	30.55065313715589	20.73775363467667
15	19.983568847298066	26.59283589636501	34.150584134695414	19.273011121641513
16	24.576469462570007	26.22119106534538	30.99644534895713	18.20589412312749
17	24.074967774407583	25.44493666509991	28.453597591339214	22.026497969153294
18	23.50369537974517	25.214486140027038	30.906467444178197	20.375351036049597
19	24.44880094256802	26.936218586012938	28.387412032920974	20.227568438498064
20	25.725113659670274	27.508445468151667	27.709679364376527	19.05676150780154
21	25.506256665407207	26.13626496013618	30.161036005362824	18.196442369093788
22	24.61504472472232	23.79784048372494	29.773258007975322	21.813856783577414
23	21.538335825476853	27.191136582734625	30.025242701721034	21.245284890067488
24	20.953397963775572	26.508840998449774	31.525347811744343	21.012413226030315
25	22.001867536228037	27.98089348874252	29.390950853439847	20.626288121589596
26	21.636368673348542	28.667495906761935	28.090473307059323	21.6056621128302
27	21.13200352275719	28.138989207074662	29.828082837407248	20.9009244327609
28	20.04840415514007	27.809132303371502	30.984339654135646	21.158123887352783
29	21.32250525491916	26.642911568597732	30.575353410628193	21.45922976585491
30	22.860859704541895	26.374025462466644	29.87487600392876	20.8902388290627
31	23.29158963836332	26.774747241240192	28.87743659118533	21.05622652921116
32	24.63741697995972	26.225777261268128	28.758824062117117	20.377981696655034
33	23.636485539863937	26.566668971404717	29.04409941662191	20.752746072109435
34	21.938987763703356	26.530049244569724	30.587482385632033	20.94348060609489
35	21.912099153090246	26.919293893445207	29.909796277452283	21.258810676012263
36	23.77707456106962	26.4350893807681	28.950117320478945	20.83771873768333
37	22.125834507236526	28.410645654905288	29.367717231455533	20.095802606402653
38	21.888702569829487	27.986853215423867	28.845682422461277	21.278761792285366
39	21.664840336361387	26.996886741214464	29.48632976052375	21.851943161900397
40	22.772232050339202	26.406245234837677	29.471314042908638	21.350208671914487
41	20.63546051343511	26.3620827289216	29.893989033637304	23.108467724005987
42	21.952117786548197	26.819189109344453	29.072640920181797	22.156052183925546
43	21.679343889964823	26.512612387990313	29.854552404738076	21.95349131730679
44	22.00158817403985	26.82254145560271	29.622588667812515	21.55328170254492
45	21.393742612907136	27.323321458112314	28.693336909169343	22.589599019811203
46	22.09256712665979	27.36995166335739	28.973979507386687	21.563501702596135
47	21.347671132038442	27.05250309684626	29.937732496271096	21.662093274844203
48	21.281601974531945	27.053946468151896	30.195467395057012	21.468984162259147
49	21.94217714868517	26.404848423896738	30.065005253173148	21.587969174244947
50	21.17381473025602	27.371557995939472	29.909935958546374	21.544691315258135
51	20.778191311416904	26.81581348290385	29.340409577560138	23.06558562811911
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1939.0
1	3431.0
2	4923.0
3	21643.0
4	38363.0
5	26474.0
6	14585.0
7	14885.5
8	15186.0
9	15900.5
10	16615.0
11	17121.5
12	17628.0
13	17704.5
14	17781.0
15	17336.5
16	16892.0
17	16670.0
18	16448.0
19	16475.0
20	16502.0
21	17772.5
22	19043.0
23	21778.0
24	24513.0
25	30618.0
26	43257.5
27	49792.0
28	59627.0
29	69462.0
30	78948.0
31	88434.0
32	101243.5
33	114053.0
34	130247.5
35	146442.0
36	155982.5
37	165523.0
38	182250.0
39	198977.0
40	214905.0
41	230833.0
42	255802.0
43	280771.0
44	295229.5
45	309688.0
46	328628.5
47	347569.0
48	359777.5
49	371986.0
50	357168.5
51	342351.0
52	318421.0
53	294491.0
54	271019.5
55	247548.0
56	228882.0
57	210216.0
58	189730.5
59	169245.0
60	148985.0
61	128725.0
62	111443.5
63	94162.0
64	79622.5
65	65083.0
66	54279.5
67	43476.0
68	35142.0
69	26808.0
70	22335.0
71	17862.0
72	14357.5
73	10853.0
74	8573.0
75	5048.5
76	3804.0
77	3033.0
78	2262.0
79	1554.0
80	846.0
81	659.5
82	473.0
83	319.0
84	165.0
85	156.5
86	148.0
87	79.0
88	10.0
89	6.0
90	2.0
91	1.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4295499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.69488223708441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11835862644627	14.417186328386556
2	8.737515478761946	3.266936458403135
3	2.9268630373459255	1.6415207942177177
4	1.434601097783914	1.0727879432104914
5	0.8756622502522401	0.8185201323962981
6	0.6394498216119068	0.7172663466935537
7	0.46174637371510907	0.6042605856003312
8	0.35853654143877184	0.5362238735911499
9	0.2917094798122884	0.49081269352786955
>10	3.998934641256649	18.70226758414119
>50	1.989127810451679	27.04107138752041
>100	1.1627348126763108	29.255555908374177
>500	0.0031315976625553798	0.39946321721348566
>1k	0.001503166878026537	0.4754310139446592
>5k	0.0	0.0
>10k+	1.2526390650221143E-4	0.5606957327788875
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23943	0.5573974059824016	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.312072939604922E-5	0.0	0.0	0.09204984100799465	0.0
2	9.312072939604922E-5	0.0	0.0	0.2405075638476461	0.0
3	9.312072939604922E-5	0.0	0.0	0.3561867899398882	0.0
4	9.312072939604922E-5	0.0	0.0	0.5502969503659528	0.0
5	9.312072939604922E-5	0.0	0.0	0.8775464736460188	0.0
6	9.312072939604922E-5	0.0	0.0	1.3311608267165236	0.0
7	9.312072939604922E-5	0.0	0.0	1.5645679349477208	0.0
8	9.312072939604922E-5	0.0	0.0	2.232476366540884	0.0
9	9.312072939604922E-5	0.0	0.0	2.5022238394188894	0.0
10	9.312072939604922E-5	0.0	0.0	2.9686422927813507	0.0
11	9.312072939604922E-5	0.0	0.0	3.4258417939336034	0.0
12	9.312072939604922E-5	0.0	0.0	3.833733868870648	0.0
13	9.312072939604922E-5	0.0	0.0	4.030521250266849	0.0
14	9.312072939604922E-5	0.0	0.0	4.1342111824493495	0.0
15	9.312072939604922E-5	0.0	0.0	4.256082937046429	0.0
16	9.312072939604922E-5	0.0	0.0	4.462345352658678	0.0
17	9.312072939604922E-5	0.0	0.0	4.707485672793778	0.0
18	9.312072939604922E-5	0.0	0.0	4.977116744760038	0.0
19	9.312072939604922E-5	0.0	0.0	5.15101970690716	0.0
20	9.312072939604922E-5	0.0	0.0	5.353138250061285	0.0
21	9.312072939604922E-5	0.0	0.0	5.588012009780471	0.0
22	9.312072939604922E-5	0.0	0.0	5.84942517737753	0.0
23	9.312072939604922E-5	0.0	0.0	6.111397069350965	0.0
24	9.312072939604922E-5	0.0	0.0	6.311955840287705	0.0
25	9.312072939604922E-5	0.0	0.0	6.495659759203762	0.0
26	9.312072939604922E-5	0.0	0.0	6.674172197455988	0.0
27	9.312072939604922E-5	0.0	0.0	6.863952243965136	0.0
28	9.312072939604922E-5	0.0	0.0	7.054221174303614	0.0
29	9.312072939604922E-5	0.0	0.0	7.265698350762042	0.0
30	9.312072939604922E-5	0.0	0.0	7.525668147053462	0.0
31	9.312072939604922E-5	0.0	0.0	7.757445642520229	0.0
32	9.312072939604922E-5	0.0	0.0	7.977001042253764	0.0
33	9.312072939604922E-5	0.0	0.0	8.190503594576555	0.0
34	9.312072939604922E-5	0.0	0.0	8.41429598749761	0.0
35	9.312072939604922E-5	0.0	0.0	8.647307332628875	0.0
36	9.312072939604922E-5	0.0	0.0	8.86993571643248	0.0
37	9.312072939604922E-5	0.0	0.0	9.103179863387235	0.0
38	9.312072939604922E-5	0.0	0.0	9.359308429591067	0.0
39	9.312072939604922E-5	0.0	0.0	9.728532121646403	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12895	0.0	40.32377	1
TACGGGA	2715	0.0	38.95027	4
CGACGGT	880	0.0	38.607956	28
GGGCGAT	7115	0.0	38.32748	7
ATAGGGA	9540	0.0	37.735847	4
TAGGGAC	6285	0.0	37.696896	5
CGTTAGG	645	0.0	37.67442	2
TAAGGGA	8095	0.0	37.46757	4
TTAGGGA	9295	0.0	37.350727	4
TAGGGAT	9520	0.0	37.153362	5
AGGGCGA	3680	0.0	37.051632	6
ACGGGAC	2320	0.0	37.047413	5
TAGGGAG	9630	0.0	36.98598	5
CGTAAGG	685	0.0	36.788322	2
AAGGGAT	9085	0.0	36.777657	5
AGGGATC	7240	0.0	36.453728	6
GACCGAT	2790	0.0	36.451614	9
TATGGGA	7610	0.0	36.307487	4
AGGGATG	11015	0.0	36.134815	6
GGACCGA	3210	0.0	36.09813	8
>>END_MODULE