FastQCFastQC Report
Thu 26 May 2016
SRR1547460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547460_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5083373
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543751.0696637842629293No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT4650.044.0322628
CGTTTTT334150.043.155021
TACGGGA24800.039.556454
ACGGGAT26850.038.7150845
ACGGGAC15750.038.1428575
ATAGGGA98550.037.6255724
GACCGAT28600.037.368889
GCGATCG3150.037.1428579
TTAGGGA92900.037.0801934
GGGCGAT95950.036.956757
AGGGCGA46400.036.9504286
GTAGGGA96300.036.8925254
TAGGGAG96850.036.7991755
TAGGGAT99150.036.7851755
TTTCGCG2150.036.6279071
TAGGGAC64050.036.6042185
GGACCGA30450.036.5763558
AGGGATC66550.036.51396
CGAGGGA42100.036.342044
ATAGGGC25900.036.312744
Produced by FastQC (version 0.11.5)