FastQCFastQC Report
Thu 26 May 2016
SRR1547459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547459_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1443785
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90640.6277943045536558No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23250.1610350571587875No Hit
GAGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT18760.12993624396984316No Hit
AAGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT15040.10417063482443716No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT14460.10015341619423945Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT207.033888E-445.022
GTCGAAC207.033888E-445.031
GCGAATC351.2122109E-745.014
ACTTCGA207.033888E-445.011
ACGATTA253.8913044E-545.035
GTCAATC253.8913044E-545.042
TATTACG351.2122109E-745.01
TTGCGAA207.033888E-445.030
GCGATAT800.045.09
TCCCGTA207.033888E-445.014
TACTCGA207.033888E-445.026
CGTAAGT253.8913044E-545.024
ACGTATC207.033888E-445.010
TTCGCGA207.033888E-445.013
CGTCAAG253.8913044E-545.022
AGTACGG1500.045.02
ATCGACG207.033888E-445.025
AATCGCG207.033888E-445.01
CGAACAT302.1658907E-644.99999620
TAAACGG1200.044.9999962