##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547459_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1443785
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29439424845112	31.0	31.0	33.0	30.0	34.0
2	31.70582254283013	31.0	31.0	34.0	30.0	34.0
3	31.838390757626655	31.0	31.0	34.0	30.0	34.0
4	35.57154423962017	37.0	35.0	37.0	33.0	37.0
5	35.44788870919147	37.0	35.0	37.0	33.0	37.0
6	35.50111270029817	37.0	35.0	37.0	33.0	37.0
7	35.921141305665316	37.0	35.0	37.0	35.0	37.0
8	35.94613048341685	37.0	35.0	37.0	35.0	37.0
9	37.66961008737451	39.0	37.0	39.0	35.0	39.0
10	37.02827983390879	39.0	37.0	39.0	33.0	39.0
11	36.849790654425696	39.0	37.0	39.0	32.0	39.0
12	36.2701593381286	38.0	35.0	39.0	32.0	39.0
13	36.041392589616876	38.0	35.0	39.0	31.0	39.0
14	37.10943803959731	39.0	35.0	41.0	32.0	41.0
15	37.29724716630246	39.0	35.0	41.0	32.0	41.0
16	37.372860225033506	39.0	35.0	41.0	32.0	41.0
17	37.288223662110354	39.0	35.0	41.0	32.0	41.0
18	37.18512936482925	39.0	35.0	41.0	32.0	41.0
19	37.103318707425274	39.0	35.0	41.0	32.0	41.0
20	36.99837648957428	39.0	35.0	41.0	32.0	41.0
21	36.9080964270996	38.0	35.0	41.0	31.0	41.0
22	36.80498896996436	38.0	35.0	41.0	31.0	41.0
23	36.753554026395896	38.0	35.0	40.0	31.0	41.0
24	36.651233390013054	38.0	35.0	40.0	31.0	41.0
25	36.56428692637754	38.0	35.0	40.0	31.0	41.0
26	36.41336140768882	38.0	35.0	40.0	31.0	41.0
27	36.33531100544749	38.0	35.0	40.0	30.0	41.0
28	36.255735445374484	38.0	35.0	40.0	30.0	41.0
29	36.30335818698767	38.0	35.0	40.0	30.0	41.0
30	36.254092541479515	38.0	35.0	40.0	30.0	41.0
31	36.131535512559005	38.0	35.0	40.0	30.0	41.0
32	36.006573693451585	38.0	35.0	40.0	30.0	41.0
33	35.85715116862968	38.0	35.0	40.0	29.0	41.0
34	35.74119346024512	38.0	35.0	40.0	29.0	41.0
35	35.629089511249944	38.0	35.0	40.0	28.0	41.0
36	35.5454413226346	38.0	35.0	40.0	27.0	41.0
37	35.45717263997063	38.0	34.0	40.0	27.0	41.0
38	35.36525937033561	38.0	34.0	40.0	27.0	41.0
39	35.285793244839084	38.0	34.0	40.0	26.0	41.0
40	35.13960319576668	38.0	34.0	40.0	25.0	41.0
41	35.0447511229165	38.0	34.0	40.0	25.0	41.0
42	34.99293731407377	38.0	34.0	40.0	25.0	41.0
43	34.94643662318143	38.0	34.0	40.0	25.0	41.0
44	34.826096683370444	37.0	34.0	40.0	24.0	41.0
45	34.68839196971848	37.0	34.0	40.0	24.0	41.0
46	34.63008619704458	37.0	34.0	40.0	24.0	41.0
47	34.5801840301707	37.0	33.0	40.0	24.0	41.0
48	34.52355648521075	37.0	34.0	40.0	23.0	41.0
49	34.46241926602645	37.0	33.0	40.0	24.0	41.0
50	34.329431321145464	37.0	33.0	40.0	24.0	41.0
51	33.748542199842774	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	2.0
13	7.0
14	6.0
15	27.0
16	106.0
17	262.0
18	620.0
19	1238.0
20	2133.0
21	3461.0
22	5269.0
23	7887.0
24	11356.0
25	16312.0
26	21210.0
27	24945.0
28	28354.0
29	32932.0
30	38492.0
31	45643.0
32	55571.0
33	70851.0
34	111169.0
35	163445.0
36	109469.0
37	135775.0
38	199865.0
39	357149.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.85155684537518	25.467850130040137	28.986864387703154	13.69372863688153
2	28.453682508129674	27.87180916826259	30.237119792766933	13.43738853084081
3	28.74887881505903	25.817209626086985	32.061075575657036	13.372835983196943
4	24.498938553870556	29.775901536586126	30.71406061151764	15.011099298025677
5	23.03196112994663	33.25190384994996	29.924330838732914	13.791804181370496
6	21.18466392156727	41.444605671897136	26.918481629882567	10.452248776653034
7	88.04344137111828	3.776947398677781	6.622177124710396	1.5574341054935466
8	87.49536807765699	3.601090190021367	6.104717807706826	2.7988239246148146
9	81.96961458942987	7.174683211142933	8.11866032684922	2.737041872577981
10	32.94881163054056	43.76766623839422	12.472563435691603	10.810958695373618
11	36.56673258137465	23.476833462045942	24.77391024286857	15.182523713710836
12	34.50513753779129	22.53493421804493	25.68962830338312	17.270299940780657
13	23.84946512119187	32.29165007255235	27.34901664721548	16.509868159040302
14	17.68358862295979	35.785175770630666	27.492805369220484	19.038430237189054
15	16.8971834449035	27.698168356091802	38.08565679793044	17.31899140107426
16	20.70148948770073	24.30389566313544	37.15657109611195	17.838043753051874
17	19.072715120326087	25.466880456577677	28.154261195399595	27.306143227696644
18	20.458517022963946	26.360157502675257	32.66151123609124	20.51981423826955
19	24.65602565478932	27.867861212022564	27.88988665209848	19.586226481089636
20	27.735223734835866	26.174534297004055	27.81570663222017	18.27453533593991
21	23.18835560696364	28.079873388350755	29.8601938654301	18.8715771392555
22	23.61037135030493	25.939111432796437	26.538646682158358	23.911870534740284
23	19.973056930221606	30.44234425485789	26.789653584155538	22.794945230764966
24	19.74185907181471	26.682920240894592	34.73785916878205	18.83736151850864
25	19.258961687508876	28.261617900172116	31.68442669788092	20.794993714438093
26	19.00303715580921	32.95428335936445	27.874856713430322	20.16782277139602
27	18.495897934941837	33.793674265905246	28.313841742364687	19.396586056788234
28	17.515073227662015	31.319552426434683	32.47526466890846	18.690109676994844
29	18.262275892878787	27.70744951637536	31.553451518058434	22.476823072687417
30	19.385296287189576	31.290115910609963	29.70352233885239	19.621065463348074
31	24.175829503700346	29.2855238141413	26.912317277156916	19.626329405001435
32	25.60512818736862	28.882555228098365	27.690410968392108	17.821905616140906
33	25.22460061574265	29.08777969018933	25.98191558992509	19.705704104142928
34	20.450967422434783	29.26827747898752	27.68978760688053	22.59096749169717
35	20.7915305949293	27.53741034849372	29.07288827630153	22.598170780275456
36	25.915146645795602	28.14269437624023	26.837236846206324	19.104922131757846
37	20.2165834940798	32.468407692281055	28.04780490169935	19.2672039119398
38	21.18632621893149	31.01472864727089	26.475479382318007	21.323465751479617
39	21.424242529185438	29.849665982123376	27.670186350460767	21.055905138230415
40	23.50599292830996	27.537202561323188	27.589357141125582	21.367447369241265
41	19.15208981946758	27.228707875480072	28.836357213851095	24.782845091201253
42	21.239450472196346	27.87942803118193	26.716027663398634	24.165093833223093
43	21.275051340746717	27.55555709471978	28.26044043953913	22.908951124994374
44	20.338416038399068	28.537420737852244	28.576346201131052	22.547817022617632
45	19.94375893917723	31.22113056999484	26.292280360302954	22.542830130524973
46	22.062980291386875	30.272028037415545	26.843401198931975	20.82159047226561
47	20.89022950092985	28.268197827238822	28.38074921127453	22.4608234605568
48	21.945580540038854	25.94215897796417	30.75014631679925	21.362114165197728
49	21.38476296678522	25.737003778263386	31.095765643776602	21.782467611174795
50	20.18174451182136	29.204694604806118	29.045598894572255	21.56796198880027
51	18.93273582978075	28.90818231246342	28.172269416845307	23.986812440910523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1205.0
1	1836.5
2	2468.0
3	8147.5
4	13827.0
5	9800.0
6	5773.0
7	6067.0
8	6361.0
9	7054.5
10	7748.0
11	8129.0
12	8510.0
13	8641.0
14	8772.0
15	8738.5
16	8705.0
17	8473.0
18	8241.0
19	8304.5
20	8368.0
21	8030.0
22	7692.0
23	8307.5
24	8923.0
25	11022.5
26	14001.5
27	14881.0
28	20043.5
29	25206.0
30	25768.5
31	26331.0
32	29814.0
33	33297.0
34	37988.5
35	42680.0
36	47049.0
37	51418.0
38	60181.0
39	68944.0
40	81604.5
41	94265.0
42	104008.5
43	113752.0
44	115575.5
45	117399.0
46	122793.5
47	128188.0
48	128332.0
49	128476.0
50	120031.5
51	111587.0
52	97827.5
53	84068.0
54	76713.5
55	69359.0
56	64760.5
57	60162.0
58	55127.0
59	50092.0
60	44919.5
61	39747.0
62	33704.5
63	27662.0
64	23472.0
65	19282.0
66	15108.5
67	10935.0
68	8647.5
69	6360.0
70	5063.0
71	3766.0
72	3047.0
73	2328.0
74	2061.0
75	1489.5
76	1185.0
77	850.0
78	515.0
79	376.5
80	238.0
81	173.0
82	108.0
83	64.5
84	21.0
85	13.0
86	5.0
87	5.5
88	6.0
89	5.0
90	4.0
91	6.0
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1443785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.38401792897833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79909020240963	15.274429772706888
2	8.553894487749023	3.31617688226232
3	2.9433539893846907	1.7116207950468822
4	1.4232589173655612	1.103539054871693
5	0.8130247290974789	0.7879842962764142
6	0.5429147403324474	0.6314321436306465
7	0.3567325340456759	0.4840436885053878
8	0.29068459114105993	0.45077082610848324
9	0.221141209110966	0.385794464601862
>10	3.0165203086421366	15.26780115539476
>50	1.7784476189421226	25.046983693099317
>100	1.251914514711631	33.31347861882648
>500	0.005774180523316828	0.7526379020951619
>1k	0.002887090261658414	0.839241278133252
>5k	3.6088628270730173E-4	0.6340654284404265
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9064	0.6277943045536558	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2325	0.1610350571587875	No Hit
GAGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	1876	0.12993624396984316	No Hit
AAGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	1504	0.10417063482443716	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	1446	0.10015341619423945	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10846490301533816	0.0
2	0.0	0.0	0.0	0.36030295369462906	0.0
3	0.0	0.0	0.0	0.5338745034752405	0.0
4	0.0	0.0	0.0	1.0038198208181965	0.0
5	0.0	0.0	0.0	1.506041411983086	0.0
6	0.0	0.0	0.0	2.179895206003664	0.0
7	0.0	0.0	0.0	2.434850064240867	0.0
8	0.0	0.0	0.0	3.147560059150081	0.0
9	0.0	0.0	0.0	3.354239031434736	0.0
10	0.0	0.0	0.0	3.7942629962217365	0.0
11	0.0	0.0	0.0	4.414161388295349	0.0
12	0.0	0.0	0.0	4.901491565572436	0.0
13	0.0	0.0	0.0	5.104430368787597	0.0
14	0.0	0.0	0.0	5.197311234013375	0.0
15	0.0	0.0	0.0	5.342415941431723	0.0
16	0.0	0.0	0.0	5.6356729014361555	0.0
17	0.0	0.0	0.0	5.9660545025748295	0.0
18	0.0	0.0	0.0	6.375949327635348	0.0
19	0.0	0.0	0.0	6.582420512749475	6.926239017582257E-5
20	0.0	0.0	0.0	6.796025724051711	6.926239017582257E-5
21	0.0	0.0	0.0	7.075153156460276	6.926239017582257E-5
22	0.0	0.0	0.0	7.362315026129236	6.926239017582257E-5
23	0.0	0.0	0.0	7.660281828665625	6.926239017582257E-5
24	0.0	0.0	0.0	7.884414923274587	6.926239017582257E-5
25	0.0	0.0	0.0	8.077449204694604	6.926239017582257E-5
26	0.0	0.0	0.0	8.25261378944926	6.926239017582257E-5
27	0.0	0.0	0.0	8.428401735715498	6.926239017582257E-5
28	0.0	0.0	0.0	8.626630696398703	6.926239017582257E-5
29	0.0	0.0	0.0	8.822781785376632	6.926239017582257E-5
30	0.0	0.0	0.0	9.072541964350648	6.926239017582257E-5
31	0.0	0.0	0.0	9.292796365109764	6.926239017582257E-5
32	0.0	0.0	0.0	9.513327815429582	6.926239017582257E-5
33	0.0	0.0	0.0	9.717236292107204	6.926239017582257E-5
34	0.0	0.0	0.0	9.94130012432599	6.926239017582257E-5
35	0.0	0.0	0.0	10.193553749346336	6.926239017582257E-5
36	0.0	0.0	0.0	10.425859805996046	6.926239017582257E-5
37	0.0	0.0	0.0	10.656157253330655	6.926239017582257E-5
38	0.0	0.0	0.0	10.894073563584605	6.926239017582257E-5
39	0.0	0.0	0.0	11.132890284910841	6.926239017582257E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTT	20	7.033888E-4	45.0	22
GTCGAAC	20	7.033888E-4	45.0	31
GCGAATC	35	1.2122109E-7	45.0	14
ACTTCGA	20	7.033888E-4	45.0	11
ACGATTA	25	3.8913044E-5	45.0	35
GTCAATC	25	3.8913044E-5	45.0	42
TATTACG	35	1.2122109E-7	45.0	1
TTGCGAA	20	7.033888E-4	45.0	30
GCGATAT	80	0.0	45.0	9
TCCCGTA	20	7.033888E-4	45.0	14
TACTCGA	20	7.033888E-4	45.0	26
CGTAAGT	25	3.8913044E-5	45.0	24
ACGTATC	20	7.033888E-4	45.0	10
TTCGCGA	20	7.033888E-4	45.0	13
CGTCAAG	25	3.8913044E-5	45.0	22
AGTACGG	150	0.0	45.0	2
ATCGACG	20	7.033888E-4	45.0	25
AATCGCG	20	7.033888E-4	45.0	1
CGAACAT	30	2.1658907E-6	44.999996	20
TAAACGG	120	0.0	44.999996	2
>>END_MODULE